LOCUS       sequence-214470-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are 
            induced with the 7a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Bhargava R, Sandhu M, Muk S, Lee G, Vaidehi N, Stark JM
  TITLE     C-NHEJ without indels is robust and requires synergistic function of
            distinct XLF domains.
  JOURNAL   Nat Commun. 2018 Jun 27;9(1):2484. doi: 10.1038/s41467-018-04867-5.
  PUBMED    29950655
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-27"; volume: "9(1):2484. doi"; pages: " 
            10.1038/s41467-018-04867-5"
COMMENT     Sequence Label: pCMV6-AC 4-uHOM
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(58..79)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(369..388)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     716..737
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             914..967
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tacggctaag gaagatatct agggataaca
      241 gggtaatccc gggggcctat tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      301 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      361 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      421 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      481 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      541 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      601 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      661 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      721 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
      781 aaggccggcc agccacggct tcccccctga ggtggccgct caggacgatg gcaccctgcc
      841 catgagctgc gcccaggaga gcggcatgga caggcacccc gccgcttgcg ccagcgctag
      901 gatcaacgtg ggtgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
      961 gggccctgtg gtaccctcga g
//
