LOCUS       sequence-214476-          20 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA/CAS9 expression plasmid to induce a 3' double-strand break in 
            the 4-uHOM reporter 8 nts. upstream from the microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Bhargava R, Sandhu M, Muk S, Lee G, Vaidehi N, Stark JM
  TITLE     C-NHEJ without indels is robust and requires synergistic function of
            distinct XLF domains.
  JOURNAL   Nat Commun. 2018 Jun 27;9(1):2484. doi: 10.1038/s41467-018-04867-5.
  PUBMED    29950655
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-27"; volume: "9(1):2484. doi"; pages: " 
            10.1038/s41467-018-04867-5"
COMMENT     Sequence Label: 7i sgRNA for 4-uHOM reporter
FEATURES             Location/Qualifiers
     source          1..20
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcacgccgta ataggccccc
//
