LOCUS       sequence-21452-e         374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of EBFP2 blue fluorescent protein with NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pEBFP2-Nuc
FEATURES             Location/Qualifiers
     source          1..374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..31
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    161..282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(198..217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     252..271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaaggtagat ccaaaaaaga agagaaaggt aggatccacc ggatctagat aactgatcat
       61 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      121 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      241 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
      301 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      361 aggccgaaat cggc
//
