LOCUS       sequence-214524-       10387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of BRAFV600E IRES H2B-mOrange2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, 
            Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, 
            Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL
  TITLE     Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 
            Activation.
  JOURNAL   Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 
            10.1016/j.ccell.2018.05.014.
  PUBMED    29990501
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.05"; journalName: "Cancer Cell"; date: 
            "2018-07-9- 9"; volume: "34"; issue: "1"; pages: "56-68.e9"
COMMENT     Sequence Label: pHIV-BRAFV600E-mOrange2
FEATURES             Location/Qualifiers
     source          1..10387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2624..3802
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2855..3793
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3750..3770
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3848..3857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6164..6737
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6187..6739
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6331..6348)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6558..6577
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6743..7120
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      7133..7142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7139..7849
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     GDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7867..7883)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7868..7884)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8393..8573
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8635..9223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8715..8734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9394..10185,10186..10254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10017..10036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10255..10359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
     2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3781 tcttccattt caggtgtcgt gagcggccgc tgaactgaat tcatcgacgt taactattct
     3841 agagtaccca tggcggcgct gagcggtggc ggtggtggcg gcgcggagcc gggccaggct
     3901 ctgttcaacg gggacatgga gcccgaggcc ggcgccggcg ccggcgccgc ggcctcttcg
     3961 gctgcggacc ctgccattcc ggaggaggtg tggaatatca aacaaatgat taagttgaca
     4021 caggaacata tagaggccct attggacaaa tttggtgggg agcataatcc accatcaata
     4081 tatctggagg cctatgaaga atacaccagc aagctagatg cactccaaca aagagaacaa
     4141 cagttattgg aatctctggg gaacggaact gatttttctg tttctagctc tgcatcaatg
     4201 gataccgtta catcttcttc ctcttctagc ctttcagtgc taccttcatc tctttcagtt
     4261 tttcaaaatc ccacagatgt ggcacggagc aaccccaagt caccacaaaa acctatcgtt
     4321 agagtcttcc tgcccaacaa acagaggaca gtggtacctg caaggtgtgg agttacagtc
     4381 cgagacagtc taaagaaagc actgatgatg agaggtctaa tcccagagtg ctgtgctgtt
     4441 tacagaattc aggatggaga gaagaaacca attggttggg acactgatat ttcctggctt
     4501 actggagaag aattgcatgt ggaagtgttg gagaatgttc cacttacaac acacaacttt
     4561 gtacgaaaaa cgtttttcac cttagcattt tgtgactttt gtcgaaagct gcttttccag
     4621 ggtttccgct gtcaaacatg tggttataaa tttcaccagc gttgtagtac agaagttcca
     4681 ctgatgtgtg ttaattatga ccaacttgat ttgctgtttg tctccaagtt ctttgaacac
     4741 cacccaatac cacaggaaga ggcgtcctta gcagagactg ccctaacatc tggatcatcc
     4801 ccttccgcac ccgcctcgga ctctattggg ccccaaattc tcaccagtcc gtctccttca
     4861 aaatccattc caattccaca gcccttccga ccagcagatg aagatcatcg aaatcaattt
     4921 gggcaacgag accgatcctc atcagctccc aatgtgcata taaacacaat agaacctgtc
     4981 aatattgatg acttgattag agaccaagga tttcgtggtg atggaggatc aaccacaggt
     5041 ttgtctgcta ccccccctgc ctcattacct ggctcactaa ctaacgtgaa agccttacag
     5101 aaatctccag gacctcagcg agaaaggaag tcatcttcat cctcagaaga caggaatcga
     5161 atgaaaacac ttggtagacg ggactcgagt gatgattggg agattcctga tgggcagatt
     5221 acagtgggac aaagaattgg atctggatca tttggaacag tctacaaggg aaagtggcat
     5281 ggtgatgtgg cagtgaaaat gttgaatgtg acagcaccta cacctcagca gttacaagcc
     5341 ttcaaaaatg aagtaggagt actcaggaaa acacgacatg tgaatatcct actcttcatg
     5401 ggctattcca caaagccaca actggctatt gttacccagt ggtgtgaggg ctccagcttg
     5461 tatcaccatc tccatatcat tgagaccaaa tttgagatga tcaaacttat agatattgca
     5521 cgacagactg cacagggcat ggattactta cacgccaagt caatcatcca cagagacctc
     5581 aagagtaata atatatttct tcatgaagac ctcacagtaa aaataggtga ttttggtcta
     5641 gctacagaga aatctcgatg gagtgggtcc catcagtttg aacagttgtc tggatccatt
     5701 ttgtggatgg caccagaagt catcagaatg caagataaaa atccatacag ctttcagtca
     5761 gatgtatatg catttgggat tgttctgtat gaattgatga ctggacagtt accttattca
     5821 aacatcaaca acagggacca gataattttt atggtgggac gaggatacct gtctccagat
     5881 ctcagtaagg tacggagtaa ctgtccaaaa gccatgaaga gattaatggc agagtgcctc
     5941 aaaaagaaaa gagatgagag accactcttt ccccaaattc tcgcctctat tgagctgctg
     6001 gcccgctcat tgccaaaaat tcaccgcagt gcatcagaac cctccttgaa tcgggctggt
     6061 ttccaaacag aggattttag tctatatgct tgtgcttctc caaaaacacc catccaggca
     6121 gggggatatg gtgcgtttcc tgtccactga gggctaggat ccgcccctct ccctcccccc
     6181 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6241 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6301 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6361 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6421 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6481 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6541 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6601 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     6661 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6721 gaaaaacacg atgataatat ggatgccaga gccagcgaag tctgctcccg ccccgaaaaa
     6781 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
     6841 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     6901 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     6961 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
     7021 ggagatccag acggccgtgc gtctgctgct gcctggggag ttggccaagc acgccgtgtc
     7081 cgagggtact aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat
     7141 ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt
     7201 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     7261 cccctacgag ggctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt
     7321 cgcctgggac atcctgtccc ctcatttcac ctacggctcc aaggcctacg tgaagcaccc
     7381 cgccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     7441 gatgaactac ggggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     7501 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtgat
     7561 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggtgc
     7621 cctgaagggc aagatcaaga tgaggctgaa gctgaaggac ggcggccact acacctccga
     7681 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga
     7741 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     7801 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac
     7861 tctagacgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     7921 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7981 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     8041 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8101 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     8161 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     8221 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     8281 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     8341 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     8401 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8461 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8521 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
     8581 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8641 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8701 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8761 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8821 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8881 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8941 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9001 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9061 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10381 acctgac
//
