LOCUS       sequence-214547-        4712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression vector for I-Ab beta chain fused to Der p 1 
            117-127 antigenic peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Moon JJ, Pepper M
  TITLE     Generation of Allergen-Specific Tetramers for a Murine Model of 
            Airway Inflammation.
  JOURNAL   Methods Mol Biol. 2018;1799:165-181. doi: 
            10.1007/978-1-4939-7896-0_14.
  PUBMED    29956152
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1799:165-181. doi"; pages: " 
            10.1007/978-1-4939-7896-0_14"
COMMENT     Sequence Label: pR I-Ab beta Derp1-117
FEATURES             Location/Qualifiers
     source          1..4712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..431
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             1306..1323
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2096..2113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2096..2112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2105..2127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2321..2340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2440..2462
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2500..2518)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2586..2690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2691..2759,2760..3551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3722..4310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4211..4230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4464..4481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4598..4619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4634..4639,4640..4657,4658..4664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4672..4688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4677..4699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4696..4712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4696..4712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattaccg ttgcaggaca ggatgtggtg cccgatgtga ctagctcttt gctgcaggcc
       61 gtcctatcct ctggttccga taagagaccc agaactccgg ccccccaccg cccaccgcca
      121 cccccataca tatgtggtac gcaagtaaga gtgcctgcgc atgccccatg tgccccacca
      181 agagctttgc atcccataca agtccccaaa gtggagaacc gaaccaattc ttcgcgggca
      241 gaacaaaagc ttctgcacac gtctccactc gaatttggag ccggccggcg tgtgcaaaag
      301 aggtgaatcg aacgaaagac ccgtgtgtaa agccgcgttt ccaaaatgta taaaaccgag
      361 agcatctggc caatgtgcat cagttgtggt cagcagcaaa atcaagtgaa tcatctcagt
      421 gcaactaaag gggaattctt agagatggct ctgcagatcc ccagcctcct cctctcagct
      481 gctgtggtgg tgctgatggt gctgagcagc cccgggactg agggctgcca aatttaccca
      541 ccaaatgtaa acaaaattgg cgggggaggt actagtggcg gtggaagtgg agggtctgaa
      601 aggcatttcg tgtaccagtt catgggcgag tgctacttca ccaacgggac gcagcgcata
      661 cgatatgtga ccagatacat ctacaaccgg gaggagtacg tgcgctacga cagcgacgtg
      721 ggcgagcacc gcgcggtgac cgagctgggg cggccagacg ccgagtactg gaacagccag
      781 ccggagatcc tggagcgaac gcgggccgag ctggacacgg tgtgcagaca caactacgag
      841 gggccggaga cccacacctc cctgcggcgg cttgaacagc ccaatgtcgt catctccctg
      901 tccaggacag aggccctcaa ccaccacaac actctggtct gctcagtgac agatttctac
      961 ccagccaaga tcaaagtgcg ctggttccgg aatggccagg aggagacggt gggggtctca
     1021 tccacacagc ttattaggaa tggggactgg accttccagg tcctggtcat gctggagatg
     1081 acccctcggc ggggagaggt ctacacctgt cacgtggagc atcccagcct gaagagcccc
     1141 atcactgtgg agtggagggc acagtctgag tctgcctgga gcaagggagg tggaggatcc
     1201 actacagctc catcagctca gttgaaaaag aaattgcaag cactgaagaa aaagaacgct
     1261 cagctgaagt ggaaacttca agccctcaag aagaaactcg cccagcatca tcatcatcat
     1321 cattgagtcg acctgcaggc atgcaagcta ttcgatgcac actcacattc ttctcctaat
     1381 acgataataa aactttccat gaaaaatatg gaaaaatata tgaaaattga gaaatccaaa
     1441 aaactgataa acgctctact taattaaaat agataaatgg gagcggcagg aatggcggag
     1501 catggccaag ttcctccgcc aatcagtcgt aaaacagaag tcgtggaaag cggatagaaa
     1561 gaatgttcga tttgacgggc aagcatgtct gctatgtggc ggattgcgga ggaattgcac
     1621 tggagaccag caaggttctc atgaccaaga atatagcggt gagtgagcgg gaagctcggt
     1681 ttctgtccag atcgaactca aaacttgtcc agccagtcgc tgtcgaaact aattaagtta
     1741 atgagttttt catgttagtt tcgcgctgag caacaattaa gtttatgttt cagttcggct
     1801 tagatttcgc tgaaggactt gccactttca atcaatactt tagaacaaaa tcaaaactca
     1861 ttctaatagc ttggtgttca tctttttttt taatgataag cattttgtcg tttatacttt
     1921 ttatatttcg atattaaacc acctatgaag tctattttaa tcgccagata agcaatatat
     1981 tgtgtaaata tttgtattct ttatcaggaa attcagggag acggggaagt tactatctac
     2041 taaaagccaa acaatttctt acagttttac tctctctact ctagagtagc ttggcactgg
     2101 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2161 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2221 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     2281 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     2341 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2401 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2461 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     2521 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4681 gataacaatt tcacacagga aacagctatg ac
//
