LOCUS       sequence-21459-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector expressing tTA-VP16 and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Kafri T, van Praag H, Gage FH, Verma IM
  TITLE     Lentiviral vectors: regulated gene expression.
  JOURNAL   Mol Ther. 2000 Jun . 1(6):516-21.
  PUBMED    10933976
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Jun . 1(6):516-21."
COMMENT     Sequence Label: pCL-CTIG
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(160..184)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(181..219)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(252..270,271..293,294..312,313..335,336..354,355..377,
                     378..396,397..419,420..438,439..461,462..480,481..503,
                     504..522)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 cgggtangct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag aggtaccggt
       61 tgttggttta aaccaacgat cgaattcggg gccgcggagg ctggatcggt cccggtgtct
      121 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
      181 agctctgctt atataggcct cccaccgtac acgcctacct cgacccgggt accgagctcg
      241 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      301 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      361 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      421 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
      481 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgaggaat
      541 tcctgcagcc cgggggatcc tcgcgccccc tacccaccgt actcgtcaat tccaagggca
      601 tcggtaaaca tctgctcaaa ctcgaagtcg gccatatcca gagcgccgta gggggcggag
      661 tcgtgggggg taaatcccgg acccggggaa tccccgtccc ccaacatgtc cagatcgaaa
      721 tcgtctagcg cgtcggcatg cgccatcgcc acgtcctcgc ngtctaagtg gagctcgtcc
      781 cc
//
