LOCUS       sequence-214595-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ulp1_R1 in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lau YK, Baytshtok V, Howard TA, Fiala BM, Johnson JM, Carter LP, 
            Baker D, Lima CD, Bahl CD
  TITLE     Discovery and engineering of enhanced SUMO protease enzymes.
  JOURNAL   J Biol Chem. 2018 Aug 24;293(34):13224-13233. doi: 
            10.1074/jbc.RA118.004146. Epub 2018 Jul 5.
  PUBMED    29976752
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004146"; journalName: "J Biol Chem"; date: 
            "2018-08-24- 24"; volume: "293"; issue: "34"; pages: "13224-13233"
COMMENT     Sequence Label: pCDB325
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             694..711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggccacc atcaccatca ccacggttct ctggttccag aactgaacga gaaagacgac
       61 gaccaggttc agaaagcgct ggactcccgt gaaaacaccc agctgatgaa ccgtgacaat
      121 atcgaaatca ccgttcgtga cttcaaaacc ctcgaaccgc gtcgctggct caacgacacc
      181 atcatcgaat tcttcatgaa gtacatcgag aagtccaccc cgaacactgt ggcgttcaac
      241 tccttctttt acactaacct gtctgaacgt ggttaccagg gcgttcgccg ttggatgaaa
      301 cgcaaaaaaa cccagatcga caagctcgac aaaatcttca ctccgatcaa cctgaatcag
      361 tctcactggg cgctgggtat cattgacctg aagaaaaaga ccattggcta cgttgattct
      421 ctgtctaacg gtccgaacga ctcttccaaa cagatcctga ccgatctgca aaagtacgtg
      481 aaggaggaat ccaagcacac tatcggcgaa gacttcgacc tgcgtcacct cgactgcccg
      541 cagcagccga acggctacga ttgcggtatc tacgtgtgca tgaataccct gtacggttct
      601 gctgatgcgc cactcgattt tgaccaaaaa gatgcggaac gtatgcgtcg ttttatcgct
      661 cacctgattc tgactgatgc tctgaaactc gagcaccacc accaccacca ctga
//
