LOCUS       sequence-214596-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ulp1_R2 in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lau YK, Baytshtok V, Howard TA, Fiala BM, Johnson JM, Carter LP, 
            Baker D, Lima CD, Bahl CD
  TITLE     Discovery and engineering of enhanced SUMO protease enzymes.
  JOURNAL   J Biol Chem. 2018 Aug 24;293(34):13224-13233. doi: 
            10.1074/jbc.RA118.004146. Epub 2018 Jul 5.
  PUBMED    29976752
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004146"; journalName: "J Biol Chem"; date: 
            "2018-08-24- 24"; volume: "293"; issue: "34"; pages: "13224-13233"
COMMENT     Sequence Label: pCDB326
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             694..711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggccacc atcaccatca ccacggttct ctggttccgg agctgaatga aaaggacgac
       61 gaccaggttc agaaagcgct caactctcgc gaaaacactc aactgatgaa tcgcgacaac
      121 atcgaaatca ccgttcgtga ctttaaaacc ctggaaccgc gtcgctggct caacgacacc
      181 atcatcgagt ttttcatgaa gtacatcgag aagtctaccc cgaataccgt tgcgttcaac
      241 tccttctttt acaccaacct gtctgaacgt ggttaccagg gtgttcgtcg ttggatgaaa
      301 cgcaagaaga cccagatcga caaactggac aaaatcttca ccccaattaa cctcaaccag
      361 tctcactggg ctctcggcat cattgacctc aagaaaaaga ccattggcta cgttgactcc
      421 ctctctaacg gtccgaatga cgattctaag cgtattctga ctgacctgca gaagtacgtg
      481 cgtgaagagt ccaagcacac tatcggtgag gacttcgacc tgcgtcatct ggactgcccg
      541 caacagccaa acggttacga ctgcggtatc tacgtttgca tgaataccct gtacggttct
      601 gcggacgcgc cactcgactt tgaccagaaa gacgcggaac gtatgcgtcg ttttattgcg
      661 catctgatcc tgaccgacgc tctgaaactc gagcaccacc accaccacca ctga
//
