LOCUS       sequence-214597-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ulp1_R3 in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lau YK, Baytshtok V, Howard TA, Fiala BM, Johnson JM, Carter LP, 
            Baker D, Lima CD, Bahl CD
  TITLE     Discovery and engineering of enhanced SUMO protease enzymes.
  JOURNAL   J Biol Chem. 2018 Aug 24;293(34):13224-13233. doi: 
            10.1074/jbc.RA118.004146. Epub 2018 Jul 5.
  PUBMED    29976752
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004146"; journalName: "J Biol Chem"; date: 
            "2018-08-24- 24"; volume: "293"; issue: "34"; pages: "13224-13233"
COMMENT     Sequence Label: pCDB327
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             694..711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggccacc atcaccatca ccacggttct ctggtgccag agctgaacga aaaggacgac
       61 gaccaggttc agaaagcgct cgattctcgc gaaaacaccc agctgatgaa ccgtgacaac
      121 atcgaaatca ccgtgcgtga cttcaaaacc ctggagccgc gtcgctggct caacgacacc
      181 atcatcgagt ttttcatgaa gtacatcgag aagtccaccc cgaacactgt ggcgttcaac
      241 tctttcttct acaccaacct ctccgaacgt ggttaccagg gtgtgcgtcg ttggatgaag
      301 cgcaaaaaaa cccagatcga caaactggac aaaatcttca ctccgatcaa cctgaaccag
      361 tctcactggg cgctgggcat tattgacctg aaaaagaaaa ccattggcta cgtggactct
      421 ctctccaacg gtccaaacga ctcctctaag cagatcctga ccgacctgca gaaatacgtt
      481 gaagaagaat ctaaacacac catcggcgaa gactttgacc tccgtcacct cgactgcccg
      541 cagcagccaa acggttatga ttgcggtatc tatgtttgca tgaacaccct ctacggttct
      601 gcggacgctc cgctcgactt cgactctaaa gacgctgagc gtatgcgccg tttcatcgcg
      661 cacctgatcc tcaccgacgc gctgaaactc gagcaccacc accaccacca ctga
//
