LOCUS       sequence-214598-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ulp1_R4 in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lau YK, Baytshtok V, Howard TA, Fiala BM, Johnson JM, Carter LP, 
            Baker D, Lima CD, Bahl CD
  TITLE     Discovery and engineering of enhanced SUMO protease enzymes.
  JOURNAL   J Biol Chem. 2018 Aug 24;293(34):13224-13233. doi: 
            10.1074/jbc.RA118.004146. Epub 2018 Jul 5.
  PUBMED    29976752
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004146"; journalName: "J Biol Chem"; date: 
            "2018-08-24- 24"; volume: "293"; issue: "34"; pages: "13224-13233"
COMMENT     Sequence Label: pCDB328
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             694..711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggccacc atcaccatca ccacggttct ctggtgccgg aactgaatga gaaagacgat
       61 gaccaggttc agaaagcgct ggagtcccgt gaaaacaccc agctgatgaa ccgtgacaac
      121 attgaaatca ccgttcgtga cttcaaaact ctgcgtccac gccgttggct gaacgacacc
      181 atcatcgagt tcttcatgaa gtacatcgag aaatctactc cgaacaccgt tgcgttcaac
      241 tctttcttct acaccaacct gtctgagcgt ggttaccagg gcgttcgtcg ttggatgaaa
      301 cgtaaaaaga cccagatcga caagctggac aaaatcttca ccccaattaa cctgaaccag
      361 tctcactggg ctctcggtat cattgacctg aaaaagaaaa ccattggcta cgtggactct
      421 ctgtctaatg gtccgaatga ctcctctaaa cagatcctga ccgacctcca gaagtacgtt
      481 aaggaagaat ccaagcacac tatcggcgag gacttcgacc tgcgtcatct ggactgcccg
      541 cagcaaccaa acggttacga ctgcggtatc tacgtttgca tgaataccct gtacggttct
      601 gcggacgctc cgctcgattt cgatcaaaaa gatgcggaac gtatgcgtcg cttcatcgcg
      661 cacctcatcc tcaccgacgc gctgaaactc gagcaccacc accaccacca ctga
//
