LOCUS       sequence-214599-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Cth SUMO protease in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Lau YK, Baytshtok V, Howard TA, Fiala BM, Johnson JM, Carter LP, 
            Baker D, Lima CD, Bahl CD
  TITLE     Discovery and engineering of enhanced SUMO protease enzymes.
  JOURNAL   J Biol Chem. 2018 Aug 24;293(34):13224-13233. doi: 
            10.1074/jbc.RA118.004146. Epub 2018 Jul 5.
  PUBMED    29976752
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004146"; journalName: "J Biol Chem"; date: 
            "2018-08-24- 24"; volume: "293"; issue: "34"; pages: "13224-13233"
COMMENT     Sequence Label: pCDB302
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             760..789
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atgtccggcc tgcgtcctcc tgcccgccag ctcatcaccc cgctgtctga agaatggcgt
       61 tctcgtgttg aggcggcacg taacgccaac ccggctaccg aactggcgaa aactctggaa
      121 ggtcagccgc tcgttcgccg cgacttcgag gaaaagctgc tccctgcgac cgcgtggctg
      181 aacgacaacg ttatcatcgg tgcgatcttc tacatcgcgg actacgttaa caccaaaaaa
      241 ggtgcgccga accaggagcc gaaatgcacc gcgttcacct ctttcttctg gcctcgtctg
      301 ctgtctcatg gtccgggtgg ttgcggccgt ctcctccgcc gtgccaacgt tcgtaaggcg
      361 aacttcctgg atatcgacac tatcctcatc ccgatttgcg aatcttctca ctggaccctc
      421 gccgttatcc gtccaggtcg ccgtaccgtt tctcatctgg actctatggc tgcgggtcgt
      481 ggttctgagc gtgttaaagc gaaactgctg gaactggtta aattcgtact ggaagaccag
      541 tttgttgaag ccgaatggca ggcggtggac ttccaggccc cacgtcaaac caacggttgg
      601 gattgcggtg tttttaccat caccaacgcg atctgtctgg cgctgggcgt tgatcctgcg
      661 caagcctaca ccgaagcgca actgccactg cagcgtcagc gtatcgctgc ggttctcctc
      721 aatggcggtt tcaaaggtga tttcacgctc gacgacctcc accatcatca ccaccatcac
      781 catcaccact aatga
//
