LOCUS       sequence-214606-        1245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A topological variant of GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Qian Y, Rancic V, Wu J, Ballanyi K, Campbell RE
  TITLE     A bioluminescent Ca2+ indicator based on a topological variant of 
            GCaMP6s.
  JOURNAL   Chembiochem. 2018 Jun 22. doi: 10.1002/cbic.201800255.
  PUBMED    29934970
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2018 Jun 22. doi: 10.1002/cbic.201800255."
COMMENT     Sequence Label: pcDNA-ncpGCaMP6s
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             910..966
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga atttaaagag
      481 gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
      541 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
      601 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
      661 aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag
      721 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
      781 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
      841 caggtaaact acgaagagtt tgtacaaatg atgacagcga agggtggcgg aggttctgtc
      901 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
      961 agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     1021 ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     1141 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agtaa
//
