LOCUS       sequence-214609-        4104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for c-Myc-tag embedded into human CD52 with short
            polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Zotova A, Pichugin A, Atemasova A, Knyazhanskaya E, Lopatukhina E, 
            Mitkin N, Holmuhamedov E, Gottikh M, Kuprash D, Filatov A, Mazurov D
  TITLE     Isolation of gene-edited cells via knock-in of short 
            glycophosphatidylinositol-anchored epitope tags.
  JOURNAL   Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.1038/s41598-019-40219-z.
  PUBMED    30816313
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-28"; volume: "9(1):3132. doi"; pages: " 
            10.1038/s41598-019-40219-z"
COMMENT     Sequence Label: pJet-CMV-CD5cmyc2-bglpA
FEATURES             Location/Qualifiers
     source          1..4104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..20)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        88..192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(193..261,262..1053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(411..430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1224..1812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1713..1732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1966..1983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2103..2124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2139..2144,2145..2162,2163..2169)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     enhancer        2685..3064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3065..3268
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3219..3239
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3265..3289
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3568..3597
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3678..3726
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3781..3800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3782..3800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
       61 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      421 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1981 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2041 taatgcagct ggcacgacag gtttcccgac tggaaagcaa ttggcagtga gcgcaacgca
     2101 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     2161 cgtataatgt gtggaattat gagcggataa taatttcaca caggaggttt aaactttaaa
     2221 catgtcaaaa gagacgtctt ttgttaagaa tgctgaggaa cttgcaaagc aaaaaatgga
     2281 tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc tgtctcagtt
     2341 tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg gtcaagagga
     2401 acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa aacccacgcc
     2461 acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt ttaatataca
     2521 gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag ctgagaatat
     2581 tgtaggagat cttctagaaa gatccccgcg gccattgcat acgttgtatc catatcataa
     2641 tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt gattattgac
     2701 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2761 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2821 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2881 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2941 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3001 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3061 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3121 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3181 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3241 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3301 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     3361 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
     3421 agtctatagg cccaccccct tggcttctta tgcgacggat ctgcctgcag gtcgaccacc
     3481 atgaagcgct tcctcttcct cctactcacc atcagcctcc tggttatggt acagatacaa
     3541 actggactct caggacaaaa cgacaccgag cagaagctga tcagcgagga agacctgtca
     3601 gcatccagca acataagcgg aggcattttc cttttcttcg tggccaatgc cataatccac
     3661 ctcttctgct tcagttgaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     3721 tgtgtggtac cgcatcttgc tgaaaaactc gagccatccg gaagatctgg cggccgctct
     3781 ccctatagtg agtcgtatta cgccggatgg atatggtgtt caggcacaag tgttaaagca
     3841 gttgatttta ttcactatga tgaaaaaaac aatgaatgga acctgctcca agttaaaaat
     3901 agagataata ccgaaaactc atcgagtagt aagattagag ataatacaac aataaaaaaa
     3961 tggtttagaa cttactcaca gcgtgatgct actaattggg acaattttcc agatgaagta
     4021 tcatctaaga atttaaatga agaagacttc agagcttttg ttaaaaatta tttggcaaaa
     4081 ataatataat tcggctgcag gggc
//
