LOCUS       sequence-214614-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyCloneYALI system-based yeast integrative vector expressing Cas9 
            carrying D-serine marker, integration into Yarrowia lipolytica ku70 
            locus, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Holkenbrink C, Dam MI, Kildegaard KR, Beder J, Dahlin J, Domenech 
            Belda D, Borodina I
  TITLE     EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering 
            of the Yeast Yarrowia lipolytica.
  JOURNAL   Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
  PUBMED    29377615
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2018 Jan 29. doi: 10.1002/biot.201700543."
COMMENT     Sequence Label: pCfB6364
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(886..919)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaaaagattc aattgagccg gcgatgcaga cccttatata aatgttgcct tggacagacg
       61 gagcaagccc gcccaaacct acgttcggta taatatgtta agctttttaa cacaaaggtt
      121 tggcttgggg taacctgatg tggtgcaaaa gaccgggcgt tggcgagcca ttgcgcgggc
      181 gaatggggcc gtgactcgtc tcaaattcga gggcgtgcct caattcgtgc ccccgtggct
      241 ttttcccgcc gtttccgccc cgtttgcacc actgcagccg cttctttggt tcggacacct
      301 tgctgcgagc taggtgcctt gtgctactta aaaagtggcc tcccaacacc aacatgacat
      361 gagtgcgtgg gccaagacac gttggcgggg tcgcagtcgg ctcaatggcc cggaaaaaac
      421 gctgctggag ctggttcgga cgcagtccgc cgcggcgtat cgatatccgc aaggttccat
      481 ggcgccattg ccctccgtcg gcgtctatcc cgcaacctct aaatagagcg ggaatataac
      541 ccaagcttct ttttttttcc tttaacacgc acacccccaa ctatcatgtt gctgctgctg
      601 tttgactcta ctctgtggag gggtgctccc acccaaccca acctacaggt ggatccggcg
      661 ctgtgattgg ctgataagtc tcctatccgg actaattctg accaatggga catgcgcgca
      721 ggacccaaat gccgcaatta cgtaacccca acgaaatgcc tacccctctt tggagcccag
      781 cggccccaaa tccccccaag cagcccggtt ctaccggctt ccatctccaa gcaccccttt
      841 ctccacaccc cacaaaaaga cccgtgcagg acatcctact gcgtcataac ttcgtatagc
      901 atacattata cgaagttatc gtacgaagct tcagctgttc tcagatgcat tcttgggcgg
      961 tctataccag ctcattga
//
