LOCUS       sequence-214633-        9516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of osTIR1 and selection with
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Zotova A, Pichugin A, Atemasova A, Knyazhanskaya E, Lopatukhina E, 
            Mitkin N, Holmuhamedov E, Gottikh M, Kuprash D, Filatov A, Mazurov D
  TITLE     Isolation of gene-edited cells via knock-in of short 
            glycophosphatidylinositol-anchored epitope tags.
  JOURNAL   Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.1038/s41598-019-40219-z.
  PUBMED    30816313
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-28"; volume: "9(1):3132. doi"; pages: " 
            10.1038/s41598-019-40219-z"
COMMENT     Sequence Label: pUCHR-osTIR1-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             350..607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    654..779
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1272..1505
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1690..1734
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2029..2146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2206..2509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2510..2713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2664..2684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2710..2734
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2832..4559
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     misc_feature    join(4640..5214,5215..5217,5218..5226)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4807..4824)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5034..5053
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5227..5229,5230..5232,5233..5946)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5227..5229,5230..5232,5233..5943)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5272..5293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5533..5552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5880..5901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6031..6664
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6713..6729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6713..6729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6726..6748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6737..6753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6761..6767,6768..6785,6786..6791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6806..6827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6944..6961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      7104..7239
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(7302..7890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7382..7401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8061..8852,8853..8921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8684..8703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8922..9026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9094..9112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9150..9172)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9272..9291
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9485..9507
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9499..9516
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9500..9516
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattgatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
       61 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
      121 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
      181 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
      241 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
      301 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
      361 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
      421 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      481 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      541 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      601 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      661 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      721 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      781 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      841 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
      901 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
      961 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1021 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1081 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1141 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1201 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1261 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1321 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1381 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1441 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1501 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1561 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1621 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1681 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1741 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1801 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1861 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     1921 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     1981 tctcgacggt atcggaccct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2041 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2101 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2161 ggacagcaga aatccacttt gggtccgata agcttgggag ttccgcgtta cataacttac
     2221 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2281 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2341 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2401 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2461 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2521 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2581 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2641 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2701 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     2761 tgacctccat agaagacacc gggaccgatc cagcctccga attcgtttaa cgcgcggcta
     2821 gcgctgccac catgacatac tttcctgaag aggtcgtcga acacattttt agcttcctgc
     2881 ctgcacagag agatagaaac acagtgagcc tggtctgcaa agtgtggtac gagatcgaac
     2941 gcctgagccg gagaggagtg ttcgtcggca actgctatgc tgtgagagca ggcagggtcg
     3001 ccgctaggtt tccaaatgtg cgcgcactga ccgtcaaggg gaaaccccac ttcgccgact
     3061 ttaacctggt gccccctgat tggggaggat acgccggccc ttggatcgag gcagccgctc
     3121 gcggctgtca tggactggag gaactgcgca tgaagcgaat ggtggtctct gacgaaagtc
     3181 tggagctgct ggctcggagc ttccctaggt ttcgcgcact ggtgctgatt tcttgcgaag
     3241 gcttcagcac cgatggactg gcagccgtgg cctcccactg taagctgctg cgggagctgg
     3301 acctccagga gaatgaagtg gaggatagag gccccagatg gctgtcttgc ttcccagact
     3361 catgtaccag cctggtgtcc ctgaactttg cctgcatcaa aggcgaagtg aatgctgggt
     3421 ccctggagcg gctggtctca agaagcccca acctgaggtc tctgcggctg aaccggagcg
     3481 tgagcgtgga cactctggct aagattctgc tgagaacccc taacctggag gatctgggaa
     3541 ccggcaatct gacagacgat ttccagacag aatcctactt taaactgact tctgccctgg
     3601 agaagtgtaa aatgctgagg agtctgtcag gattctggga tgcttcaccc gtgtgcctga
     3661 gctttatcta ccctctgtgt gcacagctga caggcctgaa cctgagctat gcaccaaccc
     3721 tggacgccag tgatctgaca aagatgatct cacgctgcgt gaaactccag cgactgtggg
     3781 tgctggactg tatttccgat aaggggctcc aggtggtcgc cagctcctgc aaggacctcc
     3841 aggagctgag agtgttccca tctgattttt acgtggccgg atatagtgct gtcactgagg
     3901 aaggcctggt ggcagtctca ctgggatgcc caaagctgaa cagcctgctg tatttctgtc
     3961 atcagatgac taatgctgca ctggtgaccg tcgccaagaa ctgccctaat ttcacccgat
     4021 ttcggctgtg tattctggaa ccaggcaaac ccgacgtggt cacatcccag ccactggatg
     4081 aagggtttgg agctatcgtg agagagtgca agggactcca gaggctgagc atttccggcc
     4141 tgctgacaga caaagtgttc atgtacatcg gcaagtatgc taagcagctg gagatgctga
     4201 gcattgcatt tgccggagac tccgataagg gcatgatgca cgtgatgaac gggtgtaaga
     4261 atctgcgaaa actggaaatc cgggacagcc ctttcgggga tgccgctctg ctgggaaact
     4321 ttgccagata cgagacaatg aggagcctgt ggatgtctag ttgcaatgtg actctgaagg
     4381 gctgtcaggt cctggctagt aaaatgccta tgctgaacgt ggaagtcatt aatgagcggg
     4441 acgggtctaa cgaaatggag gaaaatcatg gcgacctgcc aaaggtggag aaactgtatg
     4501 tgtatcggac caccgcaggg gcaagagatg atgctcccaa ctttgtgaag attctgtgag
     4561 gatccatcgg atcccgggta cgatccgccc ctctccctcc ccccccccta aggatccatc
     4621 ggatcccggg tacgatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     4681 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     4741 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     4801 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     4861 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4921 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     4981 cggcacaacc ccagtgccac gttgtgagtt gaatagttgt ggaaagagtc aaatggctct
     5041 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5101 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5161 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     5221 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5401 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5821 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5941 aagtaaagcg gccgcctcga gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6061 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     6121 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     6181 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     6241 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     6301 gccgcctagc atttcatcac atggcccgag agctgcatcc ggagtacttc aagaactgct
     6361 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6421 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcttgt
     6481 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6541 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6601 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6661 agcaggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     6721 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6781 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6841 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6901 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6961 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7021 tatccacaga atcaggggat aacgcaggaa agaacatgca tctcaattag tcagcaacca
     7081 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7141 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7201 agctattcca gaagtagtga ggaggctttt ttggaggcct agatgtgagc aaaaggccag
     7261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7981 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8041 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8101 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8161 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8221 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8281 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8341 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8401 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8461 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8521 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8581 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8641 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8701 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8761 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8821 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8881 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8941 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9001 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9061 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9121 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     9181 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9241 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     9301 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     9361 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9421 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9481 ttttcccagt cacgacgttg taaaacgacg gccagt
//
