LOCUS       sequence-214662-        4191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins in cholinergic neurons in the ventral nerve cord 
            of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Charlie NK, Schade MA, Thomure AM, Miller KG
  TITLE     Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) 
            pathway of the Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 Feb;172(2):943-61. doi: 10.1534/genetics.105.049577. 
            Epub 2005 Nov 4.
  PUBMED    16272411
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.049577"; journalName: "Genetics"; date: 
            "2006-02"; volume: "172"; issue: "2"; pages: "943-61"
COMMENT     Sequence Label: KG#65
FEATURES             Location/Qualifiers
     source          1..4191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    87..108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(123..128,129..146,147..153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     185..201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1961..1983)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2083..2102
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2180..2198)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2266..2370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2371..2439,2440..3231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2589..2608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3402..3990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3891..3910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4144..4161
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
      241 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
      301 atggatacgc taacaacttg gaaatgaaat aagcttgggc tgcagaagct tttggttttc
      361 acaattttct ggtttttggg atttttgcgg gaattttgag aggagaaagt tgggagagaa
      421 gatattggaa tggtccccgg ggagctgtac atttatacac caaggcaatc ttcgtaaaaa
      481 ataacaatag agtcatccct cagaacagaa aagaaacccg cccgggggct caaatacaca
      541 tgtcattttt cttttttcgc ctgattttta gcctaaatta gtgtttagca attttttaag
      601 gtaactcggt ggagtcattt gaacttttta aactaaaatt tcagaaaaaa ttcttttttt
      661 ttgtcaaaaa ataattttcc cgccggaatt tttcaaaaaa ataattttta ttttattttt
      721 caagttcctt gtttcctctt tattttttta ttttgccccc aaaagtttta cactaaaata
      781 tttaattttc aggtaattca tcgaaagtct ttctattttc cgcatctctt gttcaaggat
      841 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
      901 cattttcagg aggacccttg gctagcgtcg acggtaccgg tcagatatca ctcgaggagc
      961 tcccgagatc ttcagcgctc agcattcgta gaattgtaag tttaaacttt ctcatactaa
     1021 ctaacgcgct ctatttaaat tttcagaatt ccaactgagc gccggtcgct accattacca
     1081 acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt
     1141 ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct
     1201 aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat
     1261 ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac
     1321 ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa
     1381 aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac acaatgttct
     1441 gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac
     1501 cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct
     1561 tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta
     1621 tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt
     1681 tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac
     1741 aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg
     1801 gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta
     1861 aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg
     1921 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1981 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2041 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     2101 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2161 gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2221 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2581 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2881 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc g
//
