LOCUS       sequence-214664-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  snu114-30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114-30
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    270..291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(306..311,312..329,330..336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    344..360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     349..371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     368..384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     368..384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        405..423
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4015..4034)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4016..4034)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4041..4058)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4041..4057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4050..4072)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4198..4653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4285..4304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4495..4516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4756..5430)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5431..5711)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5732..5751)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5851..5873
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5911..5929)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5970..6473
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6510..6614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6615..6683,6684..7475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6833..6852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7646..8234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8135..8154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      361 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
      421 agggaacaaa agctggtacc gggccccccc tcgaggtagc gtatactctg gcatttttca
      481 tttatttgac ttatttttat ttattttatt gtacaatggc aaacaaatac acggactggt
      541 atgttcttcg tcaaattgat gatctgttct tttggcttat ccttatggga tgattaatga
      601 ttgttaaagc ctcattgcgg aaattttaat gccggaaagg cctgaaaaat ttcaaagtgg
      661 cagaaaacaa ggctttgggg cattacttgc caacggctga cgattgctag ctgcttttac
      721 agaagcgata cacaatagct cctaacatag caaaatggaa ggtgacgatt tattcgatga
      781 gtttggaaat ttgatcggag ttgatccttt tgactcggat gaagaagaaa gtgtgctgga
      841 tgagcaagaa caatatcaaa ccaatacgtt tgaggggagc ggcaacaaca atgagattga
      901 gagcagacaa cttacctcgt taggaagtaa aaaggaactt ggaatttcct tagagcaccc
      961 ttatggtaag gaagtggaag tattaatgga aactaagaac acacagtcac cacagactcc
     1021 actggtagag ccggttacag aacgaacaaa gttgcaagag catacaattt ttacacaatt
     1081 aaaaaaaaac attccgaaga ctagatataa tcgagactat atgttatcaa tggctaacat
     1141 tccggagagg ataataaatg ttggtgtcat tggacctctt cactcaggta agacctcttt
     1201 gatggatctc ctagtaatcg actcacacaa acgcatacca gatatgtcta agaatgtaga
     1261 acttggatgg aaaccgctaa gatacctgga taatttgaaa caagagattg accggggtct
     1321 ttccattaaa cttaatggtt ccactctact ctgcaccgat ttagaatcca agtctagaat
     1381 gataaacttt ttggatgcac cagggcatgt taattttatg gatgaaacgg cagtcgcact
     1441 tgcagcaagt gatctggttt taatcgtgat agatgttgta gaaggtgtta catttgtggt
     1501 agagcaattg ataaaacaaa gtattaaaaa taatgtagca atgtgttttg ttataaataa
     1561 actcgacaga ttgattctag atttaaagtt gccaccaatg gacgcatact tgaagttaaa
     1621 tcatataatt gcaaatatca attcctttac gaagggaaac gtgttttcac caatagacaa
     1681 taacataata tttgcgtcta caaagctggg cttcacattc actatcaagg aatttgtttc
     1741 ttattattac gcccactcca taccttcttc taagatagac gactttacca caaggctatg
     1801 gggtagcgta tattaccata aaggtaattt caggacaaaa ccatttgaaa atgtagagaa
     1861 gtacccgact ttcgttgaat ttatcctcat cccgctttat aaaatatttt cctacgcttt
     1921 atctatggag aaagataaat taaagaattt gttaaggtct aactttagag ttaatttaag
     1981 tcaagaggcc ctccagtacg atccgcaacc ttttttaaaa cacgttctac agttaatatt
     2041 taggcaacaa accggtcttg tagatgcgat aaccagatgt tatcaaccat ttgaattatt
     2101 cgacaataag acagctcatc tttcaattcc aggaaaatct acaccagaag gcactctttg
     2161 ggctcatgta ctgaaaactg tggattacgg tggagcagag tggtcccttg tacggatata
     2221 ctctggactt ttgaagagag gggatacagt acgtatactt gatacatcac aatctgaatc
     2281 tcgccaaaaa agacagttgc atgatatctc aaagacggaa acctccaacg aagatgaaga
     2341 tgaagatgac gaaacgcctt catgtgaggt cgaagaaatt ggcttattag gtggcagata
     2401 tgtctatccg gttcatgaag cacacaaagg acagatagta ttaataaaag gtatttcaag
     2461 tgcatatatt aaatccgcga cgttgtactc tgtaaagagt aaggaggaca tgaaacagct
     2521 aaaatttttc aaacctttag attatattac cgaggcagtt ttcaaaattg ttcttcaacc
     2581 tttactgcca agggaattac ccaagttatt ggatgccctg aacaaaattt cgaaatacta
     2641 cccaggcgtt attattaaag ttgaggaatc tggagaacat gtaatactaa gaaatggaga
     2701 gctgtatatg gactgtttgt tgtatgatct aagagcgagt tatgcaaaga tagaaattaa
     2761 aatatctgat cctttaactg ttttttctga aagttgctca aatgaatcgt tcgcttcaat
     2821 acctgtaagt aattcgatat ctcgccttgg tgaggaaaat ttaccaggtc tgtcgattag
     2881 tgtagccgct gaaccaatgg actctaaaat gattcaggat ttgagcagga acacattggg
     2941 aaagggtcaa aactgcttgg atattgacgg aataatggac aacccaagga aactatccaa
     3001 aatattgaga accgagtatg ggtgggattc gttggcatca agaaacgttt ggtcttttta
     3061 taatggcaac gtgttgatta atgacacttt gccagatgaa atcagtcctg aattattatc
     3121 caaatataag gaacaaataa tacaaggatt ttactgggct gtaaaagagg ggcctttggc
     3181 agaagaacca atttatggtg tacagtataa attgttatcg atctcagtac cttccgacgt
     3241 aaacattgac gttatgaaaa gtcaaattat tccgctaatg aaaaaagcct gttacgttgg
     3301 cttactgaca gcaatcccaa ttttattgga acccatctat gaagttgaca tcacggtcca
     3361 cgcccccttg ctgccaatag tagaggaact tatgaagaag agacgtggaa gcaggatata
     3421 caaaacaata aaagtggcag ggacaccatt gttggaggtt cgtggacaag ttccggttat
     3481 tgaatctgca ggattcgaga cagatttgag attatctacg aatggtcttg gtatgtgtca
     3541 gctgtacttt tggcacaaga tatggaggaa ggttcctggt gatgttttgg ataaagatgc
     3601 gtttattcca aaattgaaac ccgcacctat caacagttta agtcgtgatt tcgtgatgaa
     3661 aacaagaagg cggaagggta tttctacagg tggatttatg tcaaatgatg gtcctacgct
     3721 tgaaaagtat ataagcgctg aattatacgc tcaattaaga gaaaatggct tagtaccgtg
     3781 aaaatcttgg cgcataagaa ttaagcaata tgtccacaat atttttattc agcagaattt
     3841 acatatgact tcgtatttag tgatataact actcatcggg actgaaaatt ttttatttct
     3901 gcctaaatac agatgagatg agctttatag aattttacgc tgttaaaaag ttaaacggta
     3961 acaaagcaca gttagttgaa caacggtata tagagaatgg cgagctccaa ttcgccctat
     4021 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4081 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4141 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4321 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4621 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     4681 tctgtgcggt atttcacacc gcatagatcg gcaagtgcac aaacaatact taaataaata
     4741 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc
     4801 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct
     4861 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctttc
     4921 ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa gttcacctgt
     4981 cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg aagctgcact
     5041 gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca aaccgaggaa
     5101 ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa tgccgtaatc
     5161 attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa ccaagtattt
     5221 cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc ttgcaataac
     5281 cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt cagcatcgga
     5341 atctagagca cattctgcgg cctctgtgct ctgcaagccg caaactttca ccaatggacc
     5401 agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt gcttaatcac
     5461 gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt
     5521 ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc tccggatcaa gattgtacgt
     5581 aaggtgacaa gctatttttc aataaagaat atcttccact actgccatct ggcgtcataa
     5641 ctgcaaagta cacatatatt acgatgctgt tctattaaat gcttcctata ttatatatat
     5701 agtaatgtcg tgatctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5761 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5821 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5881 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5941 atgtcatgat aataatggtt tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg
     6001 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt
     6061 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa
     6121 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga
     6181 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac
     6241 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg
     6301 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac
     6361 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
     6421 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg
     6481 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8221 ggagcctatg gaaaaacgcc agcaacgcgg cc
//
