LOCUS       sequence-214665-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  snu114(G145A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114(G145A)
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    137..158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(173..178,179..196,197..203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     216..238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     235..251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        272..290
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3882..3901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3883..3901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3908..3925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3908..3924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3917..3939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4065..4520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4152..4171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4362..4383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4623..5297)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5298..5578)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5599..5618)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5718..5740
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5778..5796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5837..6340
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6377..6481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6482..6550,6551..7342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6700..6719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7513..8101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8002..8021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
       61 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      121 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
      301 tggtaccggg ccccccctcg aggtagcgta tactctggca tttttcattt atttgactta
      361 tttttattta ttttattgta caatggcaaa caaatacacg gactggtatg ttcttcgtca
      421 aattgatgat ctgttctttt ggcttatcct tatgggatga ttaatgattg ttaaagcctc
      481 attgcggaaa ttttaatgcc ggaaaggcct gaaaaatttc aaagtggcag aaaacaaggc
      541 tttggggcat tacttgccaa cggctgacga ttgctagctg cttttacaga agcgatacac
      601 aatagctcct aacatagcaa aatggaaggt gacgatttat tcgatgagtt tggaaatttg
      661 atcggagttg atccttttga ctcggatgaa gaagaaagtg tgctggatga gcaagaacaa
      721 tatcaaacca atacgtttga ggggagcggc aacaacaatg agattgagag cagacaactt
      781 acctcgttag gaagtaaaaa ggaacttgga atttccttag agcaccctta tggtaaggaa
      841 gtggaagtat taatggaaac taagaacaca cagtcaccac agactccact ggtagagccg
      901 gttacagaac gaacaaagtt gcaagagcat acaattttta cacaattaaa aaaaaacatt
      961 ccgaagacta gatataatcg agactatatg ttatcaatgg ctaacattcc ggagaggata
     1021 ataaatgttg gtgtcattgg acctcttcac tcagctaaga cctctttgat ggatctccta
     1081 gtaatcgact cacacaaacg cataccagat atgtctaaga atgtagaact tggatggaaa
     1141 ccgctaagat acctggataa tttgaaacaa gagattgacc ggggtctttc cattaaactt
     1201 aatggttcca ctctactctg caccgattta gaatccaagt ctagaatgat aaactttttg
     1261 gatgcaccag ggcatgttaa ttttatggat gaaacggcag tcgcacttgc agcaagtgat
     1321 ctggttttaa tcgtgataga tgttgtagaa ggtgttacat ttgtggtaga gcaattgata
     1381 aaacaaagta ttaaaaataa tgtagcaatg tgttttgtta taaataaact cgacagattg
     1441 attctagatt taaagttgcc accaatggac gcatacttga agttaaatca tataattgca
     1501 aatatcaatt cctttacgaa gggaaacgtg ttttcaccaa tagacaataa cataatattt
     1561 gcgtctacaa agctgggctt cacattcact atcaaggaat ttgtttctta ttattacgcc
     1621 cactccatac cttcttctaa gatagacgac tttaccacaa ggctatgggg tagcgtatat
     1681 taccataaag gtaatttcag gacaaaacca tttgaaaatg tagagaagta cccgactttc
     1741 gttgaattta tcctcatccc gctttataaa atattttcct acgctttatc tatggagaaa
     1801 gataaattaa agaatttgtt aaggtctaac tttagagtta atttaagtca agaggccctc
     1861 cagtacgatc cgcaaccttt tttaaaacac gttctacagt taatatttag gcaacaaacc
     1921 ggtcttgtag atgcgataac cagatgttat caaccatttg aattattcga caataagaca
     1981 gctcatcttt caattccagg aaaatctaca ccagaaggca ctctttgggc tcatgtactg
     2041 aaaactgtgg attacggtgg agcagagtgg tcccttgtac ggatatactc tggacttttg
     2101 aagagagggg atacagtacg tatacttgat acatcacaat ctgaatctcg ccaaaaaaga
     2161 cagttgcatg atatctcaaa gacggaaacc tccaacgaag atgaagatga agatgacgaa
     2221 acgccttcat gtgaggtcga agaaattggc ttattaggtg gcagatatgt ctatccggtt
     2281 catgaagcac acaaaggaca gatagtatta ataaaaggta tttcaagtgc atatattaaa
     2341 tccgcgacgt tgtactctgt aaagagtaag gaggacatga aacagctaaa atttttcaaa
     2401 cctttagatt atattaccga ggcagttttc aaaattgttc ttcaaccttt actgccaagg
     2461 gaattaccca agttattgga tgccctgaac aaaatttcga aatactaccc aggcgttatt
     2521 attaaagttg aggaatctgg agaacatgta atactaggaa atggagagct gtatatggac
     2581 tgtttgttgt atgatctaag agcgagttat gcaaagatag aaattaaaat atctgatcct
     2641 ttaactgttt tttctgaaag ttgctcaaat gaatcgttcg cttcaatacc tgtaagtaat
     2701 tcgatatctc gccttggtga ggaaaattta ccaggtctgt cgattagtgt agccgctgaa
     2761 ccaatggact ctaaaatgat tcaggatttg agcaggaaca cattgggaaa gggtcaaaac
     2821 tgcttggata ttgacggaat aatggacaac ccaaggaaac tatccaaaat attgagaacc
     2881 gagtatgggt gggattcgtt ggcatcaaga aacgtttggt ctttttataa tggcaacgtg
     2941 ttgattaatg atactttgcc agatgaaatc agtcctgaat tattatccaa atataaggaa
     3001 caaataatac aaggatttta ctgggctgta aaagaggggc ctttggcaga agaaccaatt
     3061 tatggtgtac agtataaatt gttatcgatc tcagtacctt ccgacgtaaa cattgacgtt
     3121 atgaaaagtc aaattattcc gctaatgaaa aaagcctgtt acgttggctt actgacagca
     3181 atcccaattt tattggaacc catctatgaa gttgacatca cggtccacgc ccccttgctg
     3241 ccaatagtag aggaacttat gaagaagaga cgtggaagca ggatatacaa aacaataaaa
     3301 gtggcaggga caccattgtt ggaggttcgt ggacaagttc cggttattga atctgcagga
     3361 ttcgagacag atttgagatt atctacgaat ggtcttggta tgtgtcagct gtacttttgg
     3421 cacaagatat ggaggaaggt tcctggtgat gttttggata aagatgcgtt tattccaaaa
     3481 ttgaaacccg cacctatcaa cagtttaagt cgtgatttcg tgatgaaaac aagaaggcgg
     3541 aagggtattt ctacaggtgg atttatgtca aatgatggtc ctacgcttga aaagtatata
     3601 agcgctgaat tatacgctca attaagagaa aatggcttag taccgtgaaa atcttggcgc
     3661 ataagaatta agcaatatgt ccacaatatt tttattcagc agaatttaca tatgacttcg
     3721 tatttagtga tataactact catcgggact gaaaattttt tatttctgcc taaatacaga
     3781 tgagatgagc tttatagaat tttacgctgt taaaaagtta aacggtaaca aagcacagtt
     3841 agttgaacaa cggtatatag agaatggcga gctccaattc gccctatagt gagtcgtatt
     3901 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3961 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4021 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     4081 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4141 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4201 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4261 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4321 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4381 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4441 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4501 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4561 tcacaccgca tagatcggca agtgcacaaa caatacttaa ataaatacta ctcagtaata
     4621 acctatttct tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt
     4681 gtctccacac ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca
     4741 acattttctg gcgtcagtcc accagctaac ataaaatgta agctttcggg gctctcttgc
     4801 cttccaaccc agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct
     4861 gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg
     4921 cagtcttttg gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct
     4981 tgccacgact catctccatg cagttggacg atatcaatgc cgtaatcatt gaccagagcc
     5041 aaaacatcct ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa
     5101 ctatttttat atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt
     5161 ctctttctat tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat
     5221 tctgcggcct ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg
     5281 aaattaataa cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg
     5341 ctcaatagtc accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaatta
     5401 attcttaatc ggcaaaaaaa gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct
     5461 atttttcaat aaagaatatc ttccactact gccatctggc gtcataactg caaagtacac
     5521 atatattacg atgctgttct attaaatgct tcctatatta tatatatagt aatgtcgtga
     5581 tctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5641 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5701 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5761 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5821 aatggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
     5881 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
     5941 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
     6001 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
     6061 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
     6121 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag
     6181 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
     6241 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
     6301 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac
     6361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8161 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8221 tgataccgct cgccgcagcc gaacgaccga gc
//
