LOCUS       sequence-214666-        8300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  myc-SNU114.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 myc-SNU114
FEATURES             Location/Qualifiers
     source          1..8300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..47
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    164..185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(200..205,206..223,224..230)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    238..254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     243..265
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     262..278
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     262..278
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        299..317
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             661..690
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3957..3976)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3958..3976)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3983..4000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3983..3999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3992..4014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4140..4595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4227..4246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4437..4458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4698..5372)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5373..5653)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5674..5693)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5793..5815
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5853..5871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5912..6415
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6452..6556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6557..6625,6626..7417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6775..6794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7588..8176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8077..8096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      121 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      181 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      241 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa
      301 ttaaccctca ctaaagggaa caaaagctgg taccgggccc cccctcgagg tagcgtatac
      361 tctggcattt ttcatttatt tgacttattt ttatttattt tattgtacaa tggcaaacaa
      421 atacacggac tggtatgttc ttcgtcaaat tgatgatctg ttcttttggc ttatccttat
      481 gggatgatta atgattgtta aagcctcatt gcggaaattt taatgccgga aaggcctgaa
      541 aaatttcaaa gtggcagaaa acaaggcttt ggggcattac ttgccaacgg ctgacgattg
      601 ctagctgctt ttacagaagc gatacacaat agctcctaac atagcaaaat ggaattccca
      661 gaacaaaaat tgatttctga agaagatttg aatagatctg aaggtgacga tttattcgat
      721 gagtttggaa atttgatcgg agttgatcct tttgactcgg atgaagaaga aagtgtgctg
      781 gatgagcaag aacaatatca aaccaatacg tttgagggga gcggcaacaa caatgagatt
      841 gagagcagac aacttacctc gttaggaagt aaaaaggaac ttggaatttc cttagagcac
      901 ccttatggta aggaagtgga agtattaatg gaaactaaga acacacagtc accacagact
      961 ccactggtag agccggttac agaacgaaca aagttgcaag agcatacaat ttttacacaa
     1021 ttaaaaaaaa acattccgaa gactagatat aatcgagact atatgttatc aatggctaac
     1081 attccggaga ggataataaa tgttggtgtc attggacctc ttcactcagg taagacctct
     1141 ttgatggatc tcctagtaat cgactcacac aaacgcatac cagatatgtc taagaatgta
     1201 gaacttggat ggaaaccgct aagatacctg gataatttga aacaagagat tgaccggggt
     1261 ctttccatta aacttaatgg ttccactcta ctctgcaccg atttagaatc caagtctaga
     1321 atgataaact ttttggatgc accagggcat gttaatttta tggatgaaac ggcagtcgca
     1381 cttgcagcaa gtgatctggt tttaatcgtg atagatgttg tagaaggtgt tacatttgtg
     1441 gtagagcaat tgataaaaca aagtattaaa aataatgtag caatgtgttt tgttataaat
     1501 aaactcgaca gattgattct agatttaaag ttgccaccaa tggacgcata cttgaagtta
     1561 aatcatataa ttgcaaatat caattccttt acgaagggaa acgtgttttc accaatagac
     1621 aataacataa tatttgcgtc tacaaagctg ggcttcacat tcactatcaa ggaatttgtt
     1681 tcttattatt acgcccactc cataccttct tctaagatag acgactttac cacaaggcta
     1741 tggggtagcg tatattacca taaaggtaat ttcaggacaa aaccatttga aaatgtagag
     1801 aagtacccga ctttcgttga atttatcctc atcccgcttt ataaaatatt ttcctacgct
     1861 ttatctatgg agaaagataa attaaagaat ttgttaaggt ctaactttag agttaattta
     1921 agtcaagagg ccctccagta cgatccgcaa ccttttttaa aacacgttct acagttaata
     1981 tttaggcaac aaaccggtct tgtagatgcg ataaccagat gttatcaacc atttgaatta
     2041 ttcgacaata agacagctca tctttcaatt ccaggaaaat ctacaccaga aggcactctt
     2101 tgggctcatg tactgaaaac tgtggattac ggtggagcag agtggtccct tgtacggata
     2161 tactctggac ttttgaagag aggggataca gtacgtatac ttgatacatc acaatctgaa
     2221 tctcgccaaa aaagacagtt gcatgatatc tcaaagacgg aaacctccaa cgaagatgaa
     2281 gatgaagatg acgaaacgcc ttcatgtgag gtcgaagaaa ttggcttatt aggtggcaga
     2341 tatgtctatc cggttcatga agcacacaaa ggacagatag tattaataaa aggtatttca
     2401 agtgcatata ttaaatccgc gacgttgtac tctgtaaaga gtaaggagga catgaaacag
     2461 ctaaaatttt tcaaaccttt agattatatt accgaggcag ttttcaaaat tgttcttcaa
     2521 cctttactgc caagggaatt acccaagtta ttggatgccc tgaacaaaat ttcgaaatac
     2581 tacccaggcg ttattattaa agttgaggaa tctggagaac atgtaatact aggaaatgga
     2641 gagctgtata tggactgttt gttgtatgat ctaagagcga gttatgcaaa gatagaaatt
     2701 aaaatatctg atcctttaac tgttttttct gaaagttgct caaatgaatc gttcgcttca
     2761 atacctgtaa gtaattcgat atctcgcctt ggtgaggaaa atttaccagg tctgtcgatt
     2821 agtgtagccg ctgaaccaat ggactctaaa atgattcagg atttgagcag gaacacattg
     2881 ggaaagggtc aaaactgctt ggatattgac ggaataatgg acaacccaag gaaactatcc
     2941 aaaatattga gaaccgagta tgggtgggat tcgttggcat caagaaacgt ttggtctttt
     3001 tataatggca acgtgttgat taatgatact ttgccagatg aaatcagtcc tgaattatta
     3061 tccaaatata aggaacaaat aatacaagga ttttactggg ctgtaaaaga ggggcctttg
     3121 gcagaagaac caatttatgg tgtacagtat aaattgttat cgatctcagt accttccgac
     3181 gtaaacattg acgttatgaa aagtcaaatt attccgctaa tgaaaaaagc ctgttacgtt
     3241 ggcttactga cagcaatccc aattttattg gaacccatct atgaagttga catcacggtc
     3301 cacgccccct tgctgccaat agtagaggaa cttatgaaga agagacgtgg aagcaggata
     3361 tacaaaacaa taaaagtggc agggacacca ttgttggagg ttcgtggaca agttccggtt
     3421 attgaatctg caggattcga gacagatttg agattatcta cgaatggtct tggtatgtgt
     3481 cagctgtact tttggcacaa gatatggagg aaggttcctg gtgatgtttt ggataaagat
     3541 gcgtttattc caaaattgaa acccgcacct atcaacagtt taagtcgtga tttcgtgatg
     3601 aaaacaagaa ggcggaaggg tatttctaca ggtggattta tgtcaaatga tggtcctacg
     3661 cttgaaaagt atataagcgc tgaattatac gctcaattaa gagaaaatgg cttagtaccg
     3721 tgaaaatctt ggcgcataag aattaagcaa tatgtccaca atatttttat tcagcagaat
     3781 ttacatatga cttcgtattt agtgatataa ctactcatcg ggactgaaaa ttttttattt
     3841 ctgcctaaat acagatgaga tgagctttat agaattttac gctgttaaaa agttaaacgg
     3901 taacaaagca cagttagttg aacaacggta tatagagaat ggcgagctcc aattcgccct
     3961 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4021 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4081 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     4141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4561 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     4621 catctgtgcg gtatttcaca ccgcatagat cggcaagtgc acaaacaata cttaaataaa
     4681 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
     4741 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
     4801 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt
     4861 tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct
     4921 gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca
     4981 ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg
     5041 aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa
     5101 tcattgacca gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat
     5161 ttcggagtgc ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata
     5221 accgggtcaa ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg
     5281 gaatctagag cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga
     5341 ccagaactac ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc
     5401 acgtatactc acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt
     5461 ttttcgaccg aattaattct taatcggcaa aaaaagaaaa gctccggatc aagattgtac
     5521 gtaaggtgac aagctatttt tcaataaaga atatcttcca ctactgccat ctggcgtcat
     5581 aactgcaaag tacacatata ttacgatgct gttctattaa atgcttccta tattatatat
     5641 atagtaatgt cgtgatctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5701 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5761 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5821 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5881 taatgtcatg ataataatgg tttcttagac ggatcgcttg cctgtaactt acacgcgcct
     5941 cgtatctttt aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt
     6001 ttgtatttgg attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa
     6061 aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta
     6121 gacaagaaaa gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc
     6181 acaggatttt cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc
     6241 tgagagcagg aagagcaaga taaaaggtag tatttgttgg cgatccccct agagtctttt
     6301 acatcttcgg aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta
     6361 atttatatat ttatattaaa aaatttaaat tataattatt tttatagcac gtgatgaaaa
     6421 ggacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6541 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6601 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6661 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6721 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6781 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6841 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6901 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6961 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7021 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7081 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7141 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7201 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7261 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7321 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7381 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7441 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7501 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7561 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7621 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7681 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7741 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7801 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7861 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7921 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7981 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8041 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8101 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8161 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8221 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8281 cgcctttgag tgagctgata
//
