LOCUS       sequence-214669-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRS314 snu114-50.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114-50
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        228..246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3838..3857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3839..3857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3864..3881)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3864..3880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3873..3895)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4021..4476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4108..4127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4318..4339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4579..5253)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5254..5534)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5555..5574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5674..5696
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5734..5752)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5793..6296
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6333..6437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6438..6506,6507..7298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6656..6675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7469..8057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7958..7977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8211..8228
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
      241 taaagggaac aaaagctggt accgggcccc ccctcgaggt agcgtatact ctggcatttt
      301 tcatttattt gacttatttt tatttatttt attgtacaat ggcaaacaaa tacacggact
      361 ggtatgttct tcgtcaaatt gatgatctgt tcttttggct tatccttatg ggatgattaa
      421 tgattgttaa agcctcattg cggaaatttt aatgccggaa aggcctgaaa aatttcaaag
      481 tggcagaaaa caaggctttg gggcattact tgccaacggc tgacgattgc tagctgcttt
      541 tacagaagcg atacacaata gctcctaaca tagcaaaatg gaaggtgacg atttattcga
      601 tgagtttgga aatttgatcg gagttgatcc ttttgactcg gatgaagaag aaagtgtgct
      661 ggatgagcaa gaacaatatc aaaccaatac gtttgagggg agcggcaaca acaatgagat
      721 tgagagcaga caacttacct cgttaggaag taaaaaggaa cttggaattt ccttagagca
      781 cccttatggt aaggaagtgg aagtattaat ggaaactaag aacacacagt caccacagac
      841 tccactggta gagccggtta cagaacgaac aaagttgcaa gagcatacaa tttttacaca
      901 attaaaaaaa aacattccga agactagata taatcgagac tatatgttat caatggctaa
      961 cattccggag aggataataa atgttggtgt cattggacct cttcactcag gtaagacctc
     1021 tttgatggat ctcctagtaa tcgactcaca caaacgcata ccagatatgt ctaagaatgt
     1081 agaacttgga tggaaaccgc taagatacct ggataatttg aaacaagaga ttgaccgggg
     1141 tctttccatt aaacttaatg gttccactct actctgcacc gatttagaat ccaagtctag
     1201 aatgataaac tttttggatg caccagggca tgttaatttt atggatgaaa cggcagtcgc
     1261 acttgcagca agtgatctgg ttttaatcgt gatagatgtt gtagaaggtg ttacatttgt
     1321 ggtagagcaa ttgataaaac aaagtattaa aaataatgta gcaatgtgtt ttgttataaa
     1381 taaactcgac agattgattc tagatttaaa gttgccacca atggacgcat acttgaagtt
     1441 aaatcatata attgcaaata tcaattcctt tacgaaggga aacgtgtttt caccaataga
     1501 caataacata atatttgcgt ctacaaagct gggcttcaca ttcactatca aggaatttgt
     1561 ttcttattat tacgcccact ccataccttc ttctaagata gacgacttta ccacaaggct
     1621 atggggtagc gtatattacc ataaaggtaa tttcaggaca aaaccatttg aaaatgtaga
     1681 gaagtacccg actttcgttg aatttatcct catcccgctt tataaaatat tttcctacgc
     1741 tttatctatg gagaaagata aattaaagaa tttgttaagg tctaacttta gagttaattt
     1801 aagtcaagag gccctccagt acgatccgca acctttttta aaacacgttc tacagttaat
     1861 atttaggcaa caaaccggtc ttgtagatgc gataaccaga tgttatcaac catttgaatt
     1921 attcgacaat aagacagctc atctttcaat tccaggaaaa tctacaccag aaggcactct
     1981 ttgggctcat gtactgaaaa ctgtggatta cggtggagca gagtggtccc ttgtacggat
     2041 atactctgga cttttgaaga gaggggatac agtacgtata cttgatacat cacaatctga
     2101 atctcgccaa aaaagacagt tgcatgatat ctcaaagacg gaaacctcca acgaagatga
     2161 agatgaagat gacgaaacgc cttcatgtga ggtcgaagaa attggcttat taggtggcag
     2221 atatgtctat ccggttcatg aagcacacaa aggacagata gtattaataa aaggtatttc
     2281 aagtgcatat attaaatccg cgacgttgta ctctgtaaag agtaaggagg acatgaaaca
     2341 gctaaaattt ttcaaacctt tagattatat taccgaggca gttttcaaaa ttgttcttca
     2401 acctttactg ccaagggaat tacccaagtt attggatgcc ctgaacaaaa tttcgaaata
     2461 ctacccaggc gttattatta aagttgagga atctggagaa catgtaatac taggaaatgg
     2521 agagctgtat atggactgtt tgttgtatga tctaagagcg agttatgcaa agatagaaat
     2581 taaaatatct gatcctttaa ctgttttttc tgaaagttgc tcaaatgaat cgttcgcttc
     2641 aatacctgta agtaattcga tatctcgcct tggtgaggaa aatttaccag gtctgtcgat
     2701 tagtgtagcc gctgaaccaa tggactctaa aatgattcag gatttgagca ggaacacatt
     2761 gggaaagggt caaaactgct tggatattga cggaataatg gacaacccaa ggaaactatc
     2821 caaaatattg agaaccgagt atgggtggga ttcgttggca tcaagaaacg tttggtcttt
     2881 ttataatggc aacgtgttga ttaatgacac tttgccagat gaaatcagtc ctgaattatt
     2941 atccaaatat aaggaacaaa taatacaagg attttactgg gctgtaaaag aggggccttt
     3001 ggcagaagaa ccaatttatg gtgtacagta taaattgtta tcgatctcag taccttccga
     3061 cgtaaacatt gacgttatga aaagtcaaat tattccgcta atgaaaaaag cctgttacgt
     3121 tggcttactg acagcaatcc caattttatt ggaacccatc tatgaagttg acatcacggt
     3181 ccacgccccc ttgctgccaa tagtagagga acttatgaag aagagacgtg gaagcaggat
     3241 atacaaaaca ataaaagtgg cagggacacc attgttggag gttcgtggac aagttccggt
     3301 tattggatct gcaggattcg agacagattt gagattatct acgaatggtc ttggtatgcg
     3361 tcagctgtac ttttggcaca agatatggag gaaggttcct ggtgatgttt tggataaaga
     3421 tgcgtttatt ccaaaattga aacccgcacc tatcaacagt ttaagtcgtg atttcgtgat
     3481 gaaaacaaga aggcggaagg gtatttctac aggtggattt atgtcaaatg atggtcctac
     3541 gcttgaaaag tatataagcg ctgaattata cgctcaatta agagaaaatg gcttagtacc
     3601 gtgaaaatct tggcgcataa gaattaagca atatgtccac aatattttta ttcagcagaa
     3661 tttacatatg acttcgtatt tagtgatata actactcatc gggactgaaa attttttatt
     3721 tctgcctaaa tacagatgag atgagcttta tagaatttta cgctgttaaa aagttaaacg
     3781 gtaacaaagc acagttagtt gaacaacggt atatagagaa tggcgagctc caattcgccc
     3841 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3901 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3961 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4021 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4081 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4141 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4201 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4261 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4321 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4381 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4441 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     4501 gcatctgtgc ggtatttcac accgcataga tcggcaagtg cacaaacaat acttaaataa
     4561 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
     4621 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
     4681 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
     4741 ttcggggctc tcttgccttc caacccagtc agaaatcgag ttccaatcca aaagttcacc
     4801 tgtcccacct gcttctgaat caaacaaggg aataaacgaa tgaggtttct gtgaagctgc
     4861 actgagtagt atgttgcagt cttttggaaa tacgagtctt ttaataactg gcaaaccgag
     4921 gaactcttgg tattcttgcc acgactcatc tccatgcagt tggacgatat caatgccgta
     4981 atcattgacc agagccaaaa catcctcctt aggttgatta cgaaacacgc caaccaagta
     5041 tttcggagtg cctgaactat ttttatatgc ttttacaaga cttgaaattt tccttgcaat
     5101 aaccgggtca attgttctct ttctattggg cacacatata atacccagca agtcagcatc
     5161 ggaatctaga gcacattctg cggcctctgt gctctgcaag ccgcaaactt tcaccaatgg
     5221 accagaacta cctgtgaaat taataacaga catactccaa gctgcctttg tgtgcttaat
     5281 cacgtatact cacgtgctca atagtcacca atgccctccc tcttggccct ctccttttct
     5341 tttttcgacc gaattaattc ttaatcggca aaaaaagaaa agctccggat caagattgta
     5401 cgtaaggtga caagctattt ttcaataaag aatatcttcc actactgcca tctggcgtca
     5461 taactgcaaa gtacacatat attacgatgc tgttctatta aatgcttcct atattatata
     5521 tatagtaatg tcgtgatcta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5581 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5641 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5701 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5761 ttaatgtcat gataataatg gtttcttaga cggatcgctt gcctgtaact tacacgcgcc
     5821 tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt atttttatgt
     5881 tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga atgaagaaaa
     5941 aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt actttacata tatatttatt
     6001 agacaagaaa agcagattaa atagatatac attcgattaa cgataagtaa aatgtaaaat
     6061 cacaggattt tcgtgtgtgg tcttctacac agacaagatg aaacaattcg gcattaatac
     6121 ctgagagcag gaagagcaag ataaaaggta gtatttgttg gcgatccccc tagagtcttt
     6181 tacatcttcg gaaaacaaaa actatttttt ctttaatttc tttttttact ttctattttt
     6241 aatttatata tttatattaa aaaatttaaa ttataattat ttttatagca cgtgatgaaa
     6301 aggacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6661 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6961 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8161 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8221 tgagcgagga agcggaagag cgcccaatac gc
//
