LOCUS       sequence-214671-        5508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pFPV-mCherry-TT
FEATURES             Location/Qualifiers
     source          1..5508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(825..843)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        911..1015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1016..1084,1085..1876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1234..1253)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2047..2635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2536..2555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2789..2806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            4119..4206
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     terminator      complement(4820..4847)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4863..4934)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4955..5508,1..157))
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5233..5252)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     join(5502..5508,1..12)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
       61 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
      121 tgatggccat gttatcctcc tcgcccttgc tcaccatatg tatatctcct tcttaaatct
      181 agaggatctt aacattttca gcgatacccg ctgcagccag gatggcttta gaacgggtct
      241 tgccgacacc gtagatcgaa gttaacgcga tcacggcgtg tttctgatca ggaatgttaa
      301 tgcctgctat acgggccact atgcactcct actatttaat atgtacgtac catgctgaaa
      361 agcccgtttt caggatactc aaatggaaac gtacagacat acaaaagatt ggctggctaa
      421 tctagccagc tcaacccaac tttgcaagaa aaatatgcga aaaaatcagc cttggcgctg
      481 tttatgcttc ggctcggcac tgcaaatcac acggatgaca ccatcacgct taacgatttt
      541 gcagttacgg cataatttct tgacggaagc acgaactttc atttttactc tccgtaactt
      601 ctcaggcgac caattaacgg ccgtagcctt tcaggttcgc cttcttcaat gcagactcat
      661 actgactgga catcatcaga gtttgcactt gagccataaa gtccataatc acgacaacaa
      721 cgataagcag tgaggtccca ccgaagtaga acggcacttt cattgcatca cgcatgaact
      781 ccgggatccc cgggtaccga gctcgaattc ttgaagacga aagggcctcg tgatacgcct
      841 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      901 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      961 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1021 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1081 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1141 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1201 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1261 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1321 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1381 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1441 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1501 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1561 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1621 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1681 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1741 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1801 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1861 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1921 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1981 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2041 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2101 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2161 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2221 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2281 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2341 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2401 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2461 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2521 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2581 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2641 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2701 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2761 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2821 ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat
     2881 cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt caggtcgagc
     2941 aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag catcacggtt
     3001 agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc
     3061 acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc ccctgtctgg
     3121 cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg
     3181 acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc gatctgctcc
     3241 gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat ggtctattgc
     3301 ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt tcagggccac
     3361 gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg gccccaggtg
     3421 aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc
     3481 gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc gggtctgctc
     3541 aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt
     3601 gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat tgatccgctc
     3661 ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg ccagcgtata
     3721 cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg
     3781 gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc
     3841 gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga actccggcat
     3901 gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc cttcgcgctg
     3961 gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg ttttcgccgt
     4021 aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc catgcaatgg
     4081 ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga agagaaaccg
     4141 gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc ctccatgata
     4201 gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag caacaaggcg
     4261 gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga aattcagcgg
     4321 gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa ggtgctggtg
     4381 ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg gctcatggcg
     4441 gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct gccgccacgc
     4501 cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg
     4561 cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct agcagcacgc
     4621 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
     4681 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
     4741 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
     4801 cgcattaaag ctttataaac gcagaaaggc ccacccgaag gtgagccagt gtgactctag
     4861 tagagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     4921 tttcgtttta tttgatgcct ggaagcttgc atgcttactt gtacagctcg tccatgccgc
     4981 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
     5041 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
     5101 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
     5161 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
     5221 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
     5281 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
     5341 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
     5401 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
     5461 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtca
//
