LOCUS       sequence-214679-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  brr2-R1107A (endogenous promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Brr2 is a splicing fidelity factor
  JOURNAL   bioRxiv 2018. 354514
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2018. 354514"
COMMENT     Sequence Label: brr2-R1107A pRS313
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    270..291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(306..311,312..329,330..336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    344..360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     349..371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     368..384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     368..384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7522..7539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7522..7538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7531..7553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8053..8345
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8346..8799
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(9489..10151)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10152..10339)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        10761..10865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10866..10934,10935..11726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11084..11103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11897..12485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12386..12405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      361 tttcacacag gaaacagcta tgacatgatt acgaattcga gctccatttt gttatttgcg
      421 agcccaatga actgagacta tattgtaata agggccgttt gttcaaattc gtactatcca
      481 tatcattcag acaggaacaa acttgataat tagtggtgtt gaatcaagta ctattaacgc
      541 attgtgatga tctctcagat atcaatgcgt gtttgcttct ttgacgatct aatattgaat
      601 attgtcgctc tttacgagaa tattaaaaat ttatgctact aaagctatgt ctactataaa
      661 ggaataatac actaatagag ataatcttga gtaaaataag gtgaaggtaa atataagtaa
      721 ttgctttgga agatttaccg tgagccttcg ttattaaaaa tgactgagca tgaaacgaag
      781 gataaggcca aaaaaattag agaaatttat cgttatgatg agatgtccaa taaggtgttg
      841 aaagttgata aacgctttat gaataccagc caaaatcctc aaagggatgc ggaaatatca
      901 caaccaaaat ccatgagtgg tagaattagt gctaaggata tgggacaggg cctgtgcaat
      961 aacattaata aaggactaaa agaaaatgat gtagccgttg aaaaaactgg aaagagcgca
     1021 tcattgaaaa aaattcagca gcataacacc attttgaatt cttcctccga tttcaggtta
     1081 cattattatc caaaggatcc ctcaaatgtc gaaacttacg agcagatttt gcagtgggtt
     1141 acagaagttt taggcaatga tataccccat gatcttatta ttggaactgc tgatatattc
     1201 atcagacaat tgaaagaaaa tgaggaaaat gaggatggta atattgaaga gcgaaaggaa
     1261 aagatccagc atgagctagg catcaatata gactccctga agtttaacga attagttaaa
     1321 cttatgaaaa acattaccga ttatgaaaca catcctgaca atagcaataa acaagcagta
     1381 gcaattcttg ccgatgatga aaaatcagat gaagaagaag taacagaaat gtccaacaat
     1441 gctaatgttc ttgggggaga aatcaatgat aatgaagatg atgatgagga atatgattac
     1501 aatgacgttg aagtcaattc taaaaaaaaa aacaagaggg cattgccaaa cattgaaaac
     1561 gatataatta aactctccga tagtaaaaca agtaatatcg agagcgttcc gatatactca
     1621 atcgatgaat tttttttgca gagaaaactt cggtcagagc ttggttacaa ggatacctct
     1681 gtaatacaag acctgtcaga aaaaattttg aatgatattg aaacacttga gcataatcca
     1741 gtagcgttgg agcagaagtt ggttgatctg cttaaattcg agaatatatc actggctgaa
     1801 tttattctca agaatagatc tacaattttt tggggcatac gactggcgaa gagcacagag
     1861 aatgaaatac cgaacttaat agagaaaatg gttgcaaaag gtttgaatga tcttgtggaa
     1921 cagtataaat ttagagaaac tacacactca aagcgtgaac tcgattccgg tgacgaccag
     1981 ccacagtctt cggaagcaaa aagaacaaaa ttcagtaatc cagcaatacc gcccgtcata
     2041 gatttggaaa agattaaatt cgacgaaagt tctaaattaa tgaccgtgac aaaagtatcc
     2101 ctaccggaag gatcattcaa aagggtgaaa ccacaatatg acgaaataca tattccagct
     2161 cctagcaaac ctgttattga ttatgagttg aaagaaatta cttctctacc agattggtgt
     2221 caggaggcgt ttccttcatc ggaaacaacg tccttaaacc ctatacaatc aaaggttttt
     2281 cacgcagcat ttgaaggtga ttcaaacatg ctaatatgtg cacccactgg ttcaggtaag
     2341 acgaatatcg cgttattgac ggtattgaaa gccttatccc atcattataa cccgaaaact
     2401 aagaaattaa acctttctgc atttaaaatt gtttatatag cacccttgaa agccttagta
     2461 caggaacaag tccgagaatt tcaaaggagg ttggcttttc taggtataaa agtcgctgaa
     2521 ttgactggtg attctaggct tagtagaaag caaattgacg agacgcaggt tttggtgtct
     2581 acgccagaga aatgggacat aactactaga aattcaaata atttggccat cgtagaactt
     2641 gttcgtttgt tgattataga tgaaatacat ttgttacatg atgacagggg accagtattg
     2701 gaaagcatcg tggctaggac attttgggct tccaaatatg gccaggaata ccccagaata
     2761 ataggccttt ctgccacctt accaaattat gaggatgtcg gtaggttttt aagagttccg
     2821 aaggaaggat tgttttattt tgactcctca tttagaccgt gtcctctatc tcaacagttt
     2881 tgtggcatta aggaacggaa ctccttgaaa aagctgaagg ctatgaatga tgcttgttat
     2941 gaaaaggtat tggaatccat caatgaaggt aatcaaataa tcgtgtttgt tcattctcga
     3001 aaagaaacat cacgaacagc aacatggttg aaaaataaat ttgctgaaga gaacattact
     3061 cataaattaa ccaaaaatga tgcaggatcg aagcagattt taaagacaga agcagccaac
     3121 gtactcgatc caagtctacg aaagttgatt gaaagcggta tagggacgca tcacgcaggg
     3181 ctgaccagaa gtgacagatc cttatctgaa gatttatttg cagacgggtt gttgcaagta
     3241 ttggtatgca ctgcgacgtt agcatggggt gttaatctac ctgctcatac agtgattata
     3301 aaaggaacag atgtctattc acctgaaaag gggtcctggg aacaactttc tccacaagat
     3361 gtacttcaaa tgttaggtag agcaggcaga ccgagatatg acacatttgg cgagggtatt
     3421 ataataacag accaatcgaa tgttcaatat tatctctcgg ttttaaatca acaattacca
     3481 atagaatcgc aatttgtttc aaaattagtt gacaacttaa atgcagaagt tgtcgcgggt
     3541 aacattaagt gcaggaatga tgctgtgaat tggttggcgt atacttacct atatgtcaga
     3601 atgttagctt cccctatgct ttacaaagta cctgatattt caagtgatgg gcaattgaaa
     3661 aagtttagag aaagcttagt ccattcggcc ctatgcattt taaaagaaca agagttggtg
     3721 ttatatgatg ctgaaaatga tgtaattgag gcgaccgatc taggaaatat agcgtcgtca
     3781 ttttacataa atcatgcttc catggatgtt tacaatagag agttagatga acatacaact
     3841 cagattgatc tttttagaat attctcaatg tcggaagagt ttaagtacgt atcggtgaga
     3901 tatgaagaga aaagagaact aaaacaactt ctagagaaag cacccattcc tatcagagaa
     3961 gatattgatg atcctttagc aaaagtgaat gtactactgc agtcttattt ttcccagcta
     4021 aagttcgaag gttttgcact taattctgat atagtgttta ttcaccaaaa tgctggtgcg
     4081 ttattgcgtg ctatgtttga aatatgcttg aagagaggat ggggacatcc cacacggatg
     4141 ttgttaaatc tttgcaaatc tgccacaact aaaatgtggc caacaaactg tcctttaaga
     4201 cagtttaaaa catgccctgt cgaagtgatt aaaaggctgg aagcctctac agtaccgtgg
     4261 ggagattatc tacagctaga aacacctgcc gaagttggtc gtgcaatcag atctgaaaaa
     4321 tatggaaaac aggtttacga tttattgaaa agatttccca aaatgtccgt aacatgcaac
     4381 gctcagccaa ttacccgtag cgttatgagg ttcaacattg aaataatagc cgactggata
     4441 tgggatatga atgtacacgg ttcccttgaa ccttttttgc tcatgctaga agacactgat
     4501 ggtgattcaa tactttatta tgacgtacta ttcatcactc cagatatagt tggtcatgaa
     4561 tttaccttat cctttactta tgagttaaag cagcacaatc aaaataactt accacctaat
     4621 ttcttcctta ctttgatatc agaaaattgg tggcacagtg aattcgagat tccagtttcc
     4681 ttcaatggat ttaaacttcc gaaaaaattt cctcctccta ctccccttct ggagaatatt
     4741 agtatttcaa catcagagct tggcaatgat gatttttccg aagtatttga atttaaaact
     4801 ttcaacaaga ttcagagcca ggtcttcgag tctctctaca attcgaatga ctcggtcttt
     4861 gtaggttcag gaaagggcac aggtaaaaca gctatggctg aattagcact gttaaatcat
     4921 tggagacaga ataaaggaag agcggtttat atcaatccgt ctggggaaaa aattgatttt
     4981 ttgctatctg attggaataa aaggttctct catcttgctg gtggtaagat aataaataag
     5041 ctaggaaatg atccttcact aaatttaaaa ttattagcaa aaagccatgt tctcttggct
     5101 acccccgtgc aatttgagct tttgtcacgt cgttggagac aaaggaagaa catccaaagt
     5161 ttagaattaa tgatatatga tgacgctcat gagattagcc aaggtgttta cggtgctgtg
     5221 tatgaaacgc taatttctag aatgatattt atagcaaccc aacttgaaaa gaaaattcga
     5281 tttgtttgcc tttcgaactg tttggcaaat gctcgtgatt ttggtgaatg ggctggtatg
     5341 acgaagtcaa acatctacaa cttttctcca agcgaaagaa ttgagcctct tgaaattaat
     5401 attcagtcat ttaaagatgt tgagcacata tcattcaatt tttcgatgct tcaaatggca
     5461 tttgaagcat ctgctgctgc agcgggaaat cgtaattcca gttcggtgtt cttaccatct
     5521 agaaaggatt gtatggaagt tgcgtctgca tttatgaagt tttcaaaagc aattgaatgg
     5581 gatatgttga acgttgaaga agagcaaatt gtaccataca ttgaaaagtt aacagatggt
     5641 catctaagag cacctttaaa acacggtgtt ggtattttgt ataaggggat ggcatcaaat
     5701 gatgaaagaa ttgtgaaaag gttatacgaa tatggtgcgg tatcggtttt actcatttct
     5761 aaggattgtt ctgcattcgc ttgtaaaact gatgaagtga ttattttagg aaccaacctt
     5821 tatgatggtg cagagcataa atatatgcca tataccataa atgaacttct agaaatggtg
     5881 ggactagcta gtggtaacga ttcaatggca ggcaaggttt taattttgac aagtcacaac
     5941 atgaaggcct actacaaaaa attcttaatt gagcctttgc ctactgagag ttacctacag
     6001 tatattattc atgatactct gaacaacgag atagcaaatt ctattataca aagcaaacaa
     6061 gattgtgttg actggtttac atattcgtat ttctatcgtc gtatccatgt gaatccaagc
     6121 tattatggcg taagggatac gtctccacat ggaatttcag tttttttgag taatttagtt
     6181 gaaacttgtt tgaatgactt agttgaatca tccttcattg aaattgacga tacagaagct
     6241 gaagtaaccg cagaagttaa tggaggagat gatgaagcaa ctgaaatcat ttctaccttg
     6301 agcaacgggc taattgcttc tcactatggc gtatcatttt ttactattca gtcattcgtt
     6361 tcttctttat cgaatacgtc aacgctcaaa aacatgctgt atgttctttc aacagcagta
     6421 gaattcgaaa gtgttccact cagaaaagga gacagagcat tacttgtcaa attaagtaaa
     6481 agactaccat tgagattccc tgaacacacc tcttctggtt cggtgagctt taaagttttc
     6541 ttattgttac aagcatattt ttcacgtctt gaattacctg tcgactttca aaatgattta
     6601 aaagacattc tagaaaaggt tgttccacta attaacgtag tggttgatat cctttcagca
     6661 aacgggtatt tgaatgctac taccgccatg gatttggcgc agatgttgat tcagggtgta
     6721 tgggacgttg ataatccgtt gagacagatt ccacacttca acaacaaaat tttggaaaaa
     6781 tgtaaagaaa ttaacgtcga gacggtctat gatataatgg cccttgagga tgaagagagg
     6841 gatgaaattt taacgcttac tgattcacag cttgcgcagg tcgccgcgtt tgttaacaat
     6901 taccccaacg ttgaacttac ttattcgttg aataattctg attcgttaat ttcaggtgta
     6961 aaacagaaaa ttaccattca attgacaaga gatgttgaac ctgagaactt acaagtaaca
     7021 tctgaaaagt atccgtttga taagctagaa agttggtggt tggttttagg tgaagtatct
     7081 aaaaaagagc tttatgcgat caagaaagtt actttaaata aagagactca gcaatatgaa
     7141 ttggaatttg acactccgac atctggtaaa cataacttaa caatttggtg tgtctgtgat
     7201 tcatatcttg acgcagataa agagttgtcc tttgaaataa atgtgaaata aaatcattgt
     7261 ttagtcctgg ataatcgaat ggatttcaat atataacatt ctcttgcggc tatttaatct
     7321 aattgtgata tatacagttt atagcaaaag cgtatcattg catcactgca tttctatatc
     7381 ttcatcttca tcttcatcct gttctccttc tttgttttcg ttttcaataa atccaccttg
     7441 atcctctaga gtcgacctgc aggggtggag ctcggtaccc ggggatcctc tagagtcgac
     7501 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     7681 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taactagctt
     7741 ttaacttgta tcctaggtta tctatgctgt ctcaccatag agaatattac ctatttcaga
     7801 atgtatgtcc atgattcgcc gggtaaatac atataataca caaatctggc ttaataaagt
     7861 ctataatata tctcataaag aagtgctaaa ttggctagtg ctatatattt ttaagaaaat
     7921 ttcttttgac taagtccata tcgactttgt aaaagttcac attagcatac atatattaca
     7981 cgagccagaa atagtaactt ttgcctaaat cacaaattgc aaaatttaat tgcttgcaaa
     8041 aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt
     8101 ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc
     8161 aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc
     8221 cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt
     8281 tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta
     8341 gagttagctt acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc
     8401 ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa
     8461 aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt
     8521 agtatttatt taagtattgt ttgtgcactt gcctgcaggc cttttgaaaa gcaagcataa
     8581 aagatctaaa cataaaatct gtaaaataac aagatgtaaa gataatgcta aatcatttgg
     8641 ctttttgatt gattgtacag gaaaatatac atcgcagggg gttgactttt accatttcac
     8701 cgcaatggaa tcaaacttgt tgaagagaat gttcacaggc gcatacgcta caatgacccg
     8761 attcttgcta gccttttctc ggtcttgcaa acaaccgccg gcagcttagt atataaatac
     8821 acatgtacat acctctctcc gtatcctcgt aatcattttc ttgtatttat cgtcttttcg
     8881 ctgtaaaaac tttatcacac ttatctcaaa tacacttatt aaccgctttt actattatct
     8941 tctacgctga cagtaatatc aaacagtgac acatattaaa cacagtggtt tctttgcata
     9001 aacaccatca gcctcaagtc gtcaagtaaa gatttcgtgt tcatgcagat agataacaat
     9061 ctatatgttg ataattagcg ttgcctcatc aatgcgagat ccgtttaacc ggaccttttc
     9121 tttgactaca acacaaaaat caagcacaga acgtcatacg aatccgcaac tacattctgg
     9181 gcattatggg ctggacttgc cacgaaggag caagcacaga aaatggtgga gaaagcacta
     9241 cccaagttag agatgcttgg aggtttagct gcatgtacgg agcgttctcg agttcaagag
     9301 aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta
     9361 tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga
     9421 cattcatagg tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat
     9481 cggtgtcact acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag
     9541 gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca
     9601 ttcttgcctc gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca
     9661 agaaaatgcg ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt
     9721 tcgacaactg cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc
     9781 aaaggcgcaa atcctgatcc aaaccttttt actccacgca cggcccctag ggcctcttta
     9841 aaagcttgac cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag
     9901 tcaccaatgc actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata
     9961 tggtccagaa accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct
    10021 gctactgctt ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc
    10081 tttaaagaga tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc
    10141 tgctctgtca tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa
    10201 gaaatcacat tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa
    10261 aaaaaaagag tcatccgcta ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa
    10321 aaaatataga gtgtactaga ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg
    10381 actgtgttat gacttccctg actaatgccg tgttcaaacg atacctggca gtgactccta
    10441 gcgctcacca agctcttaaa acgagaatta agaaaaagtc gtcatcttta tactaagttg
    10501 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
    10561 ataaaatcat tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg
    10621 tgatgccatg gtgtccgact tatgcccgag aagatgttga gcaaacttat cgcttatctg
    10681 cttctcatag agtcttgcag acaaactgcg caactcgtga aaggtaggcg gatccaggtg
    10741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    11041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11101 tatcccgtrt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12481 ggaaaaacgc cagcaacgcg gcc
//
