LOCUS       sequence-214683-        6366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the
            SYN promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Gruber TD, Krishnamurthy C, Grimm JB, Tadross MR, Wysocki LM, 
            Gartner ZJ, Lavis LD
  TITLE     Cell-Specific Chemical Delivery Using a Selective 
            Nitroreductase-Nitroaryl Pair.
  JOURNAL   ACS Chem Biol. 2018 Aug 29. doi: 10.1021/acschembio.8b00524.
  PUBMED    30111097
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Aug 29. doi: 10.1021/acschembio.8b00524."
COMMENT     Sequence Label: aav-SYN-tdNfsB(F124W)-mCherry (eNTR)
FEATURES             Location/Qualifiers
     source          1..6366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   56..185
                     /label=AAV2 ITR
     promoter        267..714
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2124..2831
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2269..2287)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2537..2556
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2859..3447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2912..2932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3330..3341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3450..3466)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3451..3467)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3496..3617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3507..3526)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3561..3580
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3779..3908
                     /label=AAV2 ITR
     rep_origin      3980..4435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4067..4086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4277..4298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4461..4565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4566..4634,4635..5426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4784..4803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5597..6185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6086..6105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tctgaactag tgcaggtatg gatatcatct cggtggcatt gaaacgtcat tctaccaaag
      781 ccttcgatgc gtcgaaaaag cttacgccag agcaagctga acagatcaag acgttgcttc
      841 agtattcacc cagttcaact aattcccaac cctggcactt catcgtggcg tccacggagg
      901 aagggaaagc gcgagtagcg aaatcagcag ccggcaacta tgtctttaac gaacgcaaaa
      961 tgctcgatgc aagccacgtg gtggtctttt gtgccaaaac cgcaatggac gatgtatggc
     1021 ttaagctggt agtggaccaa gaggatgcgg atggaaggtt cgctacaccg gaggcgaaag
     1081 ccgcgaatga taagggaaga aagttctggg cagacatgca tcgaaaagac ctccatgatg
     1141 acgcggagtg gatggccaag caggtttacc ttaatgtagg taacttcctg ttgggggtcg
     1201 ccgcgttggg gctggacgct gtgcctattg aaggttttga cgccgccatt ttggatgcag
     1261 agtttggact caaagaaaaa ggctacacta gcctcgtggt ggttccggtc ggtcaccact
     1321 cggtggagga ctttaacgcg accctcccca agtcgcggct gccgcagaat atcacactca
     1381 cggaagtggc tggttctagt gcaggtcaag gtgcacaggc tggtcaagga gcacaggctg
     1441 gttctagtgc aggtatggat atcatctcgg tggcattgaa acgtcattct accaaagcct
     1501 tcgatgcgtc gaaaaagctt acgccagagc aagctgaaca gatcaagacg ttgcttcagt
     1561 attcacccag ttcaactaat tcccaaccct ggcacttcat cgtggcgtcc acggaggaag
     1621 ggaaagcgcg agtagcgaaa tcagcagccg gcaactatgt ctttaacgaa cgcaaaatgc
     1681 tcgatgcaag ccacgtggtg gtcttttgtg ccaaaaccgc aatggacgat gtatggctta
     1741 agctggtagt ggaccaagag gatgcggatg gaaggttcgc tacaccggag gcgaaagccg
     1801 cgaatgataa gggaagaaag ttctgggcag acatgcatcg aaaagacctc catgatgacg
     1861 cggagtggat ggccaagcag gtttacctta atgtaggtaa cttcctgttg ggggtcgccg
     1921 cgttggggct ggacgctgtg cctattgaag gttttgacgc cgccattttg gatgcagagt
     1981 ttggactcaa agaaaaaggc tacactagcc tcgtggtggt tccggtcggt caccactcgg
     2041 tggaggactt taacgcgacc ctccccaagt cgcggctgcc gcagaatatc acactcacgg
     2101 aagtggctgg ttctagtgca ggtatggtga gcaagggcga ggaggataac atggccatca
     2161 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     2221 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2281 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     2341 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     2401 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     2461 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     2521 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     2581 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     2641 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     2701 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     2761 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     2821 agctgtacaa ggctggttct agatagaagc ttatcgataa tcaacctctg gattacaaaa
     2881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3241 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3421 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaccc gggcggccgc
     3481 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3541 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3601 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3661 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     3721 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag
     3781 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     3841 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     3901 gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3961 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4321 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4381 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4561 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4621 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4681 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4741 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4801 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4861 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4921 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4981 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5041 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5101 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5161 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5221 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5281 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5341 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5401 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5461 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6301 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6361 cggaag
//
