LOCUS       sequence-214689-        5950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Nakagawa M, Takizawa N, Narita M, Ichisaka T, Yamanaka S
  TITLE     Promotion of direct reprogramming by transformation-deficient Myc.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14152-7. doi: 
            10.1073/pnas.1009374107. Epub 2010 Jul 26.
  PUBMED    20660764
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009374107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14152-7"
COMMENT     Sequence Label: pMXs-Hu-L-Myc
FEATURES             Location/Qualifiers
     source          1..5950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..65
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             195..664
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    981..1005
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2150..2174)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2225..2599
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2271..2287)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2609..3025)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2625..2641)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2657..2679)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3084..3441
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3504..4097
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4127..4231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4232..4300,4301..5092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4450..4469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5256..5844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5745..5764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
       61 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      121 attaatgcaa ctatggccat ttaatgtaaa tacttaagaa aaaaaaccaa attaattttg
      181 atacatgctg catgtgaaag acccccgctg acgggtagtc aatcactcag aggagaccct
      241 cccaaggaac agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg
      301 ggtacccggg cgactcagtc aatcggagga ctggcgcccc gagtgagggg ttgtgggctc
      361 ttttattgag ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt
      421 agttcaaata aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg
      481 ttctctagaa actgctgagg gctggaccgc atctggggac catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
      661 tcagctgttc catctgttcc tgaccttgat ctgaacttct ctattctcag ttatgtattt
      721 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
      781 ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcgt cgaccactgt
      841 gctggcggcc gctcgagttt aaatagtaca taatggattt ccttacgcga aatacgggca
      901 gacatggcct gcccggttat tattattttt gacaccagac caactggtaa tggtagcgac
      961 cggcgctcag ctggaatatc accactttgt acaagaaagc tgggtcggcg cgcccaccct
     1021 tttagtagcc agtgaggtat gcaattcttt tctgcaactg ctgctgccgg catcggagct
     1081 gtcttttctc tgtagccatc ctcttctcag cccccaccag ggcttgcaag tattccaagg
     1141 ccttgcttag gatcactact ttgggggcct tggagcagct ggccagggtg ggcacctggt
     1201 ccctcagcgc caagaatcgc gaacgcaggt cattccgcct cttgcgctcc aggaagttgt
     1261 gattcttcct cttggtcaca tcctcagtat cagaactgac aggtttgggg tggcaggact
     1321 gggcagcctc actttctaca ggtgggggac tcacaatctc ttcatcctcc tcatcttcct
     1381 tttcccctgc agcatctctc tccagaacct cttcttgggg acccctctct gaagcctctt
     1441 cttgggagca gctttctgga ggaaaacggg cagcatagtt gtgctgttgc tgatggatgg
     1501 agatgtggaa atgcttcatg cagggatcca gggggtctgc tcgcaccgtg atggtgaccg
     1561 gcttccgaat acccagagac tgcctcttct ctactgtcac aacatcaatt tcttcattct
     1621 ccgagtcgct tgggctctcg gacccggagc aggcctgggt cttgggttcg cccagcggac
     1681 agggggcggc gggcgccggg ttgccggctt cgaggctggg agtgcagtcc ggggcggcgg
     1741 acgccttggg cgggttcccc cggggcgcgc caggagcgag ccggtcgctc acagctctct
     1801 ccagccgttc ccgggccgag aagccgctcc acatgcagtc acggcgtatg atggaggcgt
     1861 agttcctgcc ccagcctttc gagtggcccc gggattccgc ttcgtctccg gtgcaccctc
     1921 cgggccacgg ctccggggga ccaatcccgg gggccgggtc ccctgcgccg ggacccaagc
     1981 cccagggcgg cgacgtgggg ggcgatggca ccagctcgaa tttcttccag atgtcctcgc
     2041 tgggcgccgt ggagcggtag aaatcctccc cgcagtcata gtcgtagaaa tagtgctggt
     2101 acgagtcgta gtccatggtg aagggtgggc gcgaaggggg cggccgcgga gcctgctttt
     2161 ttgtacaaac ttgtgatttc ccgtaccacc acactgggat ccttaattaa ctagctagat
     2221 ccggcagtct agaggatggt ccacccccgg ggtcggcagc cttcacgtgg gcggcgtgta
     2281 tccaagctgc gatgccgtct actttgaggg cggtgggggt ggtcagcagg actgtgtaag
     2341 gtcctttcca gcgaggttct aggttcttag tctggtgtcg gcggacccac actgtgtcgc
     2401 cgactcggta agggtgaggt accaccggtc ggtccagttg ttcttggtag gctgccgcca
     2461 gaggtctcca gacttcgtgc tggactaagt agagagcctg taagtgagct tggagagagg
     2521 ggctgttagt aactcttgtc atgtcagggt cagggaagtt tacaaggggc gggggtgccc
     2581 catataagat ctcatatggc catatggggg cgcctagaga aggagtgagg gctggataaa
     2641 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
     2701 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
     2761 ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg
     2821 aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc
     2881 atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga
     2941 catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac
     3001 ttaagggagt ggtaacagtc tggccctaat tttcagacaa atacagaaac acagtcagac
     3061 agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa
     3121 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
     3181 ccacatatcc ctcctattag gggggtgcac caaagagtcc aaaacgatcg ggatttttgg
     3241 actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc agggttgcgg
     3301 ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
     3361 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
     3421 agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc
     3481 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
     3541 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3601 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg
     3661 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
     3721 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
     3781 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
     3841 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     3901 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
     3961 ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttt tctattctca
     4021 gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
     4081 aggtggggtc tttcattatg caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4201 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4261 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4321 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4381 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4441 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4501 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4561 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4621 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4681 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4741 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4801 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4861 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4921 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4981 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5041 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5101 ccaagtttac tcatatatac tttagattga tttgcggccg gccgcaaaag gatctaggtg
     5161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5401 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5641 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5701 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5761 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5821 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5881 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5941 cgtattaccg
//
