LOCUS       sequence-214694-        6091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, 
            Okita K, Yamanaka S
  TITLE     Suppression of induced pluripotent stem cell generation by the 
            p53-p21 pathway.
  JOURNAL   Nature. 2009 Aug 27. 460(7259):1132-5.
  PUBMED    19668191
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Aug 27. 460(7259):1132-5."
COMMENT     Sequence Label: pMXs-p53
FEATURES             Location/Qualifiers
     source          1..6091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        457..561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(562..630,631..1422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(780..799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1586..2174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2075..2094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2328..2345
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2475..2944
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    3261..3285
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4164..4175
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4571..4595)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4646..5020
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(4692..4708)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(5030..5446)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5046..5062)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5078..5100)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5505..5862
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             join(5925..6091,1..427)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
       61 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
      121 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
      181 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
      241 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
      301 ttcagctgtt ccatctgttc ctgaccttga tctgaacttt tctattctca gttatgtatt
      361 tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc
      421 tttcattatg caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      781 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1081 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1441 tcatatatac tttagattga tttgcggccg gccgcaaaag gatctaggtg aagatccttt
     1501 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1561 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1621 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1681 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1741 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1801 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1861 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1921 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1981 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2041 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2101 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2161 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2221 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2281 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2341 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2401 attaatgcaa ctatggccat ttaatgtaaa tacttaagaa aaaaaaccaa attaattttg
     2461 atacatgctg catgtgaaag acccccgctg acgggtagtc aatcactcag aggagaccct
     2521 cccaaggaac agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg
     2581 ggtacccggg cgactcagtc aatcggagga ctggcgcccc gagtgagggg ttgtgggctc
     2641 ttttattgag ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt
     2701 agttcaaata aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg
     2761 ttctctagaa actgctgagg gctggaccgc atctggggac catctgttct tggccctgag
     2821 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
     2881 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
     2941 tcagctgttc catctgttcc tgaccttgat ctgaacttct ctattctcag ttatgtattt
     3001 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
     3061 ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcgt cgaccactgt
     3121 gctggcggcc gctcgagttt aaatagtaca taatggattt ccttacgcga aatacgggca
     3181 gacatggcct gcccggttat tattattttt gacaccagac caactggtaa tggtagcgac
     3241 cggcgctcag ctggaatatc accactttgt acaagaaagc tgggtcggcg cgcccaccct
     3301 tgtgtctcag ccctgaagtc ataagacagc aaggagaggg ggaggctggt gatggggacg
     3361 ggatgcagag gcagtcagtc tgagtcaggc cccactttct tgaccattgt ttttttatgg
     3421 cgggaagtag actggccctt cttggtcttc aggtagctgg agtgagccct gctgtctcca
     3481 gactcctctg tagcatgggc atcctttaac tctaaggcct cattcagctc ccggaacatc
     3541 tcgaagcgtt tacgcccgcg gatcttgagg gtgaaatact ctccatcaag tggttttttc
     3601 ttttgcgggg gagaggcgct tgtgcaggtg ggcagcgctc tctttgcgct ccctgggggc
     3661 agttcagggc aaaggacttc ctttttgcgg aaattttctt cttctgtacg gcggtctctc
     3721 ccagggcagg cacaaacacg aacctcaaag ctgtcccgtc ccagaaggtt cccactggag
     3781 tcttccagtg tgatgatggt aaggataggt cggcggttca tgccccccat gcaggagcta
     3841 ttacacatgt acttgtagtg gatggtggta tactcagagc cggcctcggg tggctcataa
     3901 ggtaccacca cgctgtggcg aaaagtctgc ctgtcttcca gatactcggg atacaaattt
     3961 ccttccaccc ggataagatg ctggggagga gccaggccat caccatcgga gcagcgctca
     4021 tggtgggggc agcgtctcac gacctccgtc atgtgctgtg acttcttgta gatggccatg
     4081 gcgcggacac ggctcccagc tggaggtgtg gcgctgaccc acaactgcac agggcacgtc
     4141 ttcgccagct ggcagaatag cttattgagg ggaggagagt acgtgcacat aacagacttg
     4201 gctgtcccag actgcaggaa gcccaggtgg aagccatagt tgccctggta agttttttga
     4261 gaagggacaa aagatgacag gggccatgga gtggctgggg caggggccac tggcccaggg
     4321 gtctcggtga cagggtcctg tgctgcagga gctcctgaca ctcggagggc ttcacttggg
     4381 ccttcaaaaa actcctcaac atcctggggc agcaacagat cgtccatgca gtgaggtgat
     4441 ggcaggatat cttctggagg aagtagtttc cataagcctg aaaatgtctc ctggctcaga
     4501 gggagctcga ggctgatatc cgactgtgac tcctccatgg cagtcatcct ggtgaagggg
     4561 gcggccgcgg agcctgcttt tttgtacaaa cttgtgattt cccgtaccac cacactggga
     4621 tccttaatta actagctaga tccggcagtc tagaggatgg tccacccccg gggtcggcag
     4681 ccttcacgtg ggcggcgtgt atccaagctg cgatgccgtc tactttgagg gcggtggggg
     4741 tggtcagcag gactgtgtaa ggtcctttcc agcgaggttc taggttctta gtctggtgtc
     4801 ggcggaccca cactgtgtcg ccgactcggt aagggtgagg taccaccggt cggtccagtt
     4861 gttcttggta ggctgccgcc agaggtctcc agacttcgtg ctggactaag tagagagcct
     4921 gtaagtgagc ttggagagag gggctgttag taactcttgt catgtcaggg tcagggaagt
     4981 ttacaagggg cgggggtgcc ccatataaga tctcatatgg ccatatgggg gcgcctagag
     5041 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
     5101 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
     5161 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg
     5221 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
     5281 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
     5341 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
     5401 tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggccctaa ttttcagaca
     5461 aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcag acaagacgcg
     5521 cggcgcggct tcggtcccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     5581 ggttctcgtc tcctaccaga accacatatc cctcctatta ggggggtgca ccaaagagtc
     5641 caaaacgatc gggatttttg gactcaggtc gggccacaaa aacggccccc gaagtccctg
     5701 ggacgtctcc cagggttgcg gccgggtgtt ccgaactcgt cagttccacc acgggtccgc
     5761 cagatacaga gctagttagc taactagtac cgacgcaggc gcataaaatc agtcatagac
     5821 actagacaat cggacagaca cagataagtt gctggccagc ttacctcccg gtggtgggtc
     5881 ggtggtccct gggcaggggt ctcccgatcc cggacgagcc cccaaatgaa agacccccgc
     5941 tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac cacaagtcgg
     6001 atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag tcaatcggag
     6061 gactggcgcg ccgagtgagg ggttgtgggc t
//
