LOCUS       sequence-214695-        9348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT
  TITLE     Scribble associates with two polarity proteins, Lgl2 and Vangl2, via
            distinct molecular domains.
  JOURNAL   J Cell Biochem. 2006 Oct 1;99(2):647-64.
  PUBMED    16791850
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2006-10-1"; volume: "99(2)"; pages: "647-64"
COMMENT     Sequence Label: pLK45
FEATURES             Location/Qualifiers
     source          1..9348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2072..2083
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5866..6000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5903..5922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5957..5976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6009..6042
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6054..6072)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6055..6072)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   6145..6247
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      6331..6786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6418..6437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6628..6649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6812..6916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6917..6985,6986..7777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7135..7154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7948..8536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8437..8456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8690..8707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   8786..8888
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     8976..9126
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgta
      601 agcttgcacc atgctcaagt gcatcccgct gtggcgctgc aaccggcacg tggagtcggt
      661 ggacaagcgg cactgttcgc tgcaggccgt gccggaggag atctaccgct acagccgcag
      721 cctggaggag ctgctgctcg acgccaacca gctgcgcgag ctgcccaagc cttttttccg
      781 gctgctgaac ttgcgcaagc tgggcctgag cgacaacgag atccagcggt tgcctcccga
      841 ggtggccaac ttcatgcagc tggtggagct ggacgtgtcc cggaacgata tccctgagat
      901 cccggagagc atcaagttct gcaaggctct ggagatcgcg gacttcagcg ggaaccccct
      961 ctccaggctc cctgatggct tcactcagct gcgcagcctg gctcacctgg ccctgaatga
     1021 tgtgtctctg caggcactgc ccggggacgt gggcaacctc gccaacctgg tgaccctgga
     1081 gctccgggag aacctgctca agtccctgcc agcgtccctg tcatttctgg tcaagctgga
     1141 acagctggat ctgggaggca acgatctgga agtgctgcca gacactctgg gggctctgcc
     1201 caatcttcgg gagctgtggc ttgaccggaa ccagctgtca gcactgcccc cggagctcgg
     1261 gaacctgcgg cgcctggtgt gcctggacgt gtcggaaaac cggctggagg agctgcctgc
     1321 tgagctcggc gggctggtgc tgctcactga cctgctgctg tcccagaacc tgctgcggag
     1381 gctgcccgac ggcatcggtc agctgaagca gctatccatc ctaaaggtag accagaatcg
     1441 gctgtgcgag gtgactgagg ccatcgggga ctgtgagaac ctctctgagc tgatcctcac
     1501 ggagaacctg ctgatggccc tgccccgctc cctgggaaag ctgactaagc tgaccaacct
     1561 caacgtggac cggaaccacc tcgaggcgct gccgcccgag atcgggggct gtgtggcact
     1621 cagcgtcctc tccttgaggg acaaccgcct ggccgtcctg ccaccagagc tggcccacac
     1681 gacagagctg cacgtgctgg acgtggcggg gaaccgcctg cagagtctgc cgttcgcgct
     1741 cacccacctc aatctcaagg ccctgtggct ggcagagaac caggcgcagc ccatgctccg
     1801 gttccagacg gaggatgatg cccggaccgg cgagaaggtg ctcacctgct acttgctgcc
     1861 ccagcagccc ccactcagcc tcgaggatgc tgggcagcag gggagcctct cggagacctg
     1921 gagcgatgcc ccgccgagcc gcgtcagcgt catccagttc ctggaggccc ccataggtga
     1981 tgaggacgct gaggaagctg cagctgagaa gcggggccta cagcgccggg ccacacctca
     2041 ccccagcgag ctcaaggtga tgaagaggag catcgagggg cggcggagcg aggcctgccc
     2101 ttgccagcca gactctgggt cgcccttgcc tgcagaggag gagaagcggc tgagtgccga
     2161 gtctggcctg agtgaagact ctcgcccatc tgccagcaca gtctctgagg ctgagcccga
     2221 gggcccgtcg gctgaggcac agggtgggag ccagcaggaa gccacgactg ctggcgggga
     2281 ggaagacgcc gaagaggact accaggagcc cacggtgcat ttcgcagagg acgcactgct
     2341 gcccggggat gacagggaga tcgaggaggg gcagcctgag gccccctgga ccctgccagg
     2401 cgggaggcag cggctcatcc gcaaggacac acctcactac aaaaagcact tcaagatctc
     2461 caagctgccc cagcccgagg ccgttgtggc tctgctgcag ggcatgcagc ctgatgggga
     2521 gggccctgtg gctcccgggg gctggcacaa tggcccccac gcaccctggg ctcctcgggc
     2581 ccagaaggag gaggaggagg aggaagaggg tagtcctcag gaggaggagg aagaggagga
     2641 ggaggaaaac agggctgaag aggaagaggc cagcactgag gaggaggaca aggagggggc
     2701 cgtggtttct gcgccctctg tcaagggagt gtcgtttgac caggccaata acctgctgat
     2761 agagcctgct cgcattgagg aggaagagct gaccctcacc atcctgcggc agactggggg
     2821 cctgggcatc agcattgcgg gcggcaaggg ctccacaccc tataaggggg acgacgaggg
     2881 catattcatc tctcgggtgt ccgaggaagg ccctgcggcc cgggctggag tccgtgtggg
     2941 tgacaagctc ctggaggtga atggtgtggc tctgcagggc gccgagcacc acgaggccgt
     3001 ggaggcgctc cggggggccg gcactgccgt gcagatgcga gtgtggcggg agcgcatggt
     3061 ggagcctgag aacgcggtca ccatcacgcc gctgcggccc gaggatgatt acagcccccg
     3121 agagcggcgg ggaggggggc tgcgcctgcc cctgctcccg cctgagagcc ccgggcccct
     3181 ccgtcagcgc cacgtggcct gcctggcacg cagcgagagg gggctgggct tcagcattgc
     3241 tggtgggaaa ggctccacac cctacagggc tggtgatgcg ggcatcttcg tctcccgcat
     3301 tgccgagggc ggtgctgctc accgcgcggg cacactgcag gttggcgacc gcgtcctctc
     3361 tattaatgga gtggacgtga ctgaggccag gcatgaccac gccgtctccc tgctgaccgc
     3421 tgcctccccc accatcgccc tgctgttgga gcgggaggct gggggccctc ttcctcccag
     3481 ccctctgcca cattcctcac cccccaccgc tgctgttgcc accaccagca taaccactgc
     3541 cacccccggg gtgcctgggt tgccgagcct ggcccccagc ctgctggctg ccgcgttgga
     3601 agggccatac ccagtggagg agatccgtct gccaagagct gggggccctc tggggcttag
     3661 tattgtcgga ggctccgacc attccagcca cccgtttggt gtccaggagc ctggtgtgtt
     3721 catctccaag gtgctcccgc ggggcctggc cgctcgcagc ggcctgcggg ttggggaccg
     3781 catcctggca gtgaacgggc aagacgtgcg ggatgccacg caccaagaag cagtcagtgc
     3841 cctgctccgg ccctgcctgg agctgtcgct gctggtgcgg agggacccgg cacccccggg
     3901 cctacgggaa ctgtgcatcc agaaggcacc tggggagagg ctgggcatca gcatccgcgg
     3961 gggtgccagg ggccacgctg gcaacccccg cgaccccaca gacgagggca tcttcatctc
     4021 caaggtgagc cccacggggg cagccgggcg cgacggtcgg ctgcgtgtgg gtttgcggct
     4081 gttggaggtg aaccagcaga gcctgctggg cctgacgcac ggcgaggcgg tgcagctgct
     4141 ccgcagtgtg ggcgacaccc tcaccgtgct ggtctgtgac ggcttcgagg ccagcaccga
     4201 cgcagccctg gaggtgtccc caggtgtcat tgccaacccc tttgcggcag gcatcggcca
     4261 ccggaacagc ctggagagca tctcttccat cgaccgggag ctgagccctg agggcccagg
     4321 caaggagaag gagctgcctg gacagaccct gcactggggg cccgaggcca cagaagccgc
     4381 aggtcggggt ctgcagcccc tgaagctgga ctaccgcgcc ctggccgccg tgcccagcgc
     4441 tggcagcgtg cagagggtac cgtctggagc agctggaggg aagatggctg aatctccctg
     4501 ctcccctagt ggccagcagc cgccctcccc gccttctccg gatgagctgc ccgccaatgt
     4561 gaagcaggcc tacagggcct tcgcggccgt gcccacttct cacccgcctg aggatgcccc
     4621 tgcccagccc cccacgcctg ggcctgcagc ctccccggag cagctgtcct tccgggagcg
     4681 gcagaagtac tttgagctgg aggtgcgcgt gccccaggcc gagggccccc ctaagcgcgt
     4741 gtccctggtg ggtgctgacg acctgcggaa gatgcaggag gaggaagcca gaaaactaca
     4801 gcagaagaga gcgcagatgc tgcgggaggc ggcagaggct ggggccgaag cgaggctcgc
     4861 cctggacggg gagacgctgg gcgaggagga acaggaggat gagcagccac cctgggccag
     4921 cccgagcccc acctcaaggc agagcccggc gtcccccccg cccctgggag gtggcgcccc
     4981 ggtgcggacg gccaaagctg aacggcgcca ccaggagcgg ctgcgcgtgc agagtccgga
     5041 gccaccggca cccgagcgtg ccctgtcccc tgccgagctc cgggccctgg aggccgagaa
     5101 gcgtgcgctg tggagggcag ccaggatgaa gtcattggaa caggacgctc tccgagcaca
     5161 gatggtcctc agcaggtccc aggaaggccg gggcacgcgg gggcccctgg agcgactggc
     5221 cgaggcccct tcccctgcgc ccaccccgtc gcccacccct gtggaagacc ttggccccca
     5281 gaccagcacc tccccgggac gcctgtcacc ggactttgct gaggagttga ggtccctgga
     5341 accatctccc agccctggcc cgcaggagga ggatggagaa gtggctctgg tgcttctggg
     5401 caggccctca cccggcgctg tgggccctga agatgtggca ctgtgcagca gccgccgccc
     5461 cgtaaggcct gggcgccgtg gcctgggccc tgtgccctcc tagaggagca ggcacctccc
     5521 ccagacttgg ggtgggggcc ctgccagctc cagcaccacc cttgccccaa gtcttttaac
     5581 ctgggtgtta gcattttaaa gagaccccac aggagttctg gcctgtgact aactaactgc
     5641 cccaccccag ccgagacctc ggcgagactg taactagata actgatcata atcagccata
     5701 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5761 aacataaaat gaatgcaatt ctttgtagag gttttacttg ctttaaaaaa cctcccacac
     5821 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     5881 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5941 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     6001 atcgatccat aacttcgtat aatgtatgct atacgaagtt atccagatct ggttctatag
     6061 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     6121 aacgacaata tgtccatagg gcccctacgt cacccgcccc gttcccacgc cccgcgccac
     6181 gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag gtatattatt
     6241 gatgatggcc gcagcggccc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6301 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6361 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6421 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6481 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6541 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6601 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6661 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6721 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6781 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7141 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8701 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8761 tcattaatgc aggggccgct gcggccatca tcaataatat accttatttt ggattgaagc
     8821 caatatgata atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg
     8881 tgacgtagta gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac
     8941 ggatgtggca aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc
     9001 gcgcggtttt aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt
     9061 ttcgcgggaa aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg
     9121 cgtaatattt gtctagggcc gcggggactt tgaccgttta cgtggagact cgcccaggtg
     9181 tttttctcag gtgttttccg cgttccgggt caaagttggc gttttattat tatagtcagc
     9241 tgacgtgtag tgtatttata cccggtgagt tcctcaagag gccactcttg agtgccagcg
     9301 agtagagttt tctcctccga gccgctccga caccgggact cgagtgtt
//
