LOCUS       sequence-214699-        5287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5287)
  TITLE     Ramakrishnan Lab Plasmids
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMSP12::mCherry
FEATURES             Location/Qualifiers
     source          1..5287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..148)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             140..955
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(213..232)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1286..1874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1775..1794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3941
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3925..3941
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4506..4522)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4507..4523)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             4576..5286
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4721..4739)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4989..5008
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagccacc tgacgtcggg ggggggggga aagccacgtt gtgtctcaaa atctctgatg
       61 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      121 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      181 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      241 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct
      301 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      361 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      421 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc
      481 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      541 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      601 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      661 tgttgaacaa gtctggaaag aaatgcataa tcttttgcca ttctcaccgg attcagtcgt
      721 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      781 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
      841 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
      901 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
      961 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt
     1021 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     1081 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     1141 gctctcacca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg
     1201 tatgagtcag caacaccttc ttcacgaggc agacctcact agttccatga gcgtcagacc
     1261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1381 ctctttttcc gaaggtaact ggcttcagca gagcacagat accaaatact gtccttctag
     1441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1681 gagaaagcgc cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg
     1741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1981 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgagccc
     2041 accagctccg taagttcggg cgctgtgtgg ctcgtacccg cgcattcagg cggcaggggg
     2101 tctaacgggt ctaaggcggc gtgtacggcc gccacagcgg ctctcagcgg cccggaaacg
     2161 tcctcgaaac gacgcatgtg ttcctcctgg ttggtacagg tggttggggg tgctcggctg
     2221 tcgctggtgt tccaccacca gggctcgacg ggagagcggg ggagtgtgca gttgtggggt
     2281 ggcccctcag cgaaatatct gacttggagc tcgtgtcgga ccatacaccg gtgattaatc
     2341 gtggtctact accaagcgtg agccacgtcg ccgacgaatt tgagcagctc tggctgccgt
     2401 actggccgct ggcaagcgac gatctgctcg aggggatcta ccgccaaagc cgcgcgtcgg
     2461 ccctaggccg ccggtacatc gaggcgaacc caacagcgct ggcaaacctg ctggtcgtgg
     2521 acgtagacca tccagacgca gcgctccgag cgctcagcgc ccgggggtcc catccgctgc
     2581 ccaacgcgat cgtgggcaat cgcgccaacg gccacgcaca cgcagtgtgg gcactcaacg
     2641 cccctgttcc acgcaccgaa tacgcgcggc gtaagccgct cgcatacatg gcggcgtgcg
     2701 ccgaaggcct tcggcgcgcc gtcgatggcg accgcagtta ctcaggcctc atgaccaaaa
     2761 accccggcca catcgcctgg gaaacggaat ggctccactc agatctctac acactcagcc
     2821 acatcgaggc cgagctcggc gcgaacatgc caccgccgcg ctggcgtcag cagaccacgt
     2881 acaaagcggc tccgacgccg ctagggcgga attgcgcact gttcgattcc gtcaggttgt
     2941 gggcctatcg tcccgccctc atgcggatct acctgccgac ccggaacgtg gacggactcg
     3001 gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga atttccgtgc aacgacgtgt
     3061 gtcccggacc gctaccggac agcgaggtcc gcgccatcgc caacagcatt tggcgttgga
     3121 tcacaaccaa gtcgcgcatt tgggcggacg ggatcgtggt ctacgaggcc acactcagtg
     3181 cgcgccagtc ggccatctcg cggaagggcg cagcagcgcg cacggcggcg agcacagttg
     3241 cgcggcgcgc aaagtccgcg tcagccatgg aggcattgct atgagcgacg gctacagcga
     3301 cggctacaac cggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg ccgaaggcgc
     3361 tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg aacgcagcga
     3421 gtggctcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg acgacgaggg
     3481 ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg ttaagcgact
     3541 cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa aggcccacaa
     3601 cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg cgggggttcc
     3661 gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg ctagttttct
     3721 ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc ccgttttgaa taccagccag
     3781 acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga atatcgggga
     3841 gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt gtagtgcctg
     3901 tggtggcatc cgtggcgcgg ccgctctaga actagtggat ctgacccgct ccacaaccgc
     3961 tgagcagggt ggcagtcaga gcgaccaggc cgaccgatac gaggcggatt ttgaacaggt
     4021 tcactcgaag cctcccgctg ggttcggagc atcgtcgatc acatgacggc gtgctgacac
     4081 cgattcgact atgcacagta gtaggtagcc gccgcggacg gtagcgcagg cggcctcggc
     4141 gccgtcgtgg cgtcgcgatg gttggcgcca gacccgcctg atcggcgtcc cgaccagggc
     4201 cgccggggtg gtgagcctcg gcttggtcca gccgtagtga gacgggatgt cttcccggtt
     4261 gtcgggtttg ggtgggcggt cacatgttcg ctggctgtgg gcgtgtcggg ggtcgtcgaa
     4321 gcggccgcga acagggctgg acgatgcacg tcttgcacct cctgtcaacc cccaatgttg
     4381 agccgcgatg cccctgacct gcaccgttgg accgcagtcg ttaggggggc gtgttagcat
     4441 ggagtgacga aaggggccta aatcagatga tcccccgggc tgcaggaatt cgatatcaag
     4501 cttatcgata ccgtcgacct cgaggggggg cccggtacca gatctttaaa tctagattta
     4561 gaaggagata tacatatggt gagcaagggc gaggaggata acatggccat catcaaggag
     4621 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4681 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4741 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     4801 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     4861 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4921 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     4981 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     5041 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     5101 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5161 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     5221 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     5281 aagtaaa
//
