LOCUS       sequence-214700-        7247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of 
            CAMERA 1.0.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT007a
FEATURES             Location/Qualifiers
     source          1..7247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(3564..3639)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(join(3666..3672,3673..3690,3691..3696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3711..3732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(3824..4615)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4616..4687)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(4715..5665)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5713..5935
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      6430..6476
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    6510..6528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(6591..7247,1..3450)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(6591..7247,1..3447)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(6591..7247,1..3447)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
       61 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
      121 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
      181 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
      241 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
      301 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
      361 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
      421 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
      481 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
      541 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg
      601 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
      661 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
      721 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
      781 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
      841 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
      901 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
      961 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     1021 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     1081 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     1141 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     1201 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     1261 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     1321 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     1381 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     1441 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     1501 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     1561 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     1621 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     1681 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     1741 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     1801 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggac
     1861 catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     1921 tccgataaaa acagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     1981 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     2041 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     2101 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     2161 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     2221 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     2281 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     2341 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     2401 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     2461 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     2521 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     2581 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     2641 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     2701 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     2761 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     2821 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     2881 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     2941 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     3001 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     3061 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     3121 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     3181 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     3241 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
     3301 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     3361 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     3421 atcgacctct ctcagctcgg tggagactga acggagccaa tgtacgcaaa aaccccgctt
     3481 cagcggggtt ttttcgccaa aaaaaacccc gccctgtcag gggcggggtt tttttttatg
     3541 ttgaaaatct ccttctaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     3601 actagcctta ttttaacttg ctatttctag ctctaaaacc cggcaagctg cccgtgccct
     3661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3721 taactcacat taattgcgtt gcgcacttaa ttaacggcac tcctcagcaa atataatgac
     3781 cctcttgata acccaagagg gcatttttta atgcccatgg cgtttatttg ccgactacct
     3841 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     3901 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     3961 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     4021 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     4081 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     4141 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     4201 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     4261 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     4321 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     4381 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     4441 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     4501 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     4561 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatactct
     4621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4681 ttaaatgtat ttagaaaaat aggccaaatg ccgttcagat ccttccgtat ttagccagta
     4741 tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat
     4801 gcttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag
     4861 ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg
     4921 ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat
     4981 ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat
     5041 caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc
     5101 attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt
     5161 catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata
     5221 accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat
     5281 attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt
     5341 ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa
     5401 ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata
     5461 caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg
     5521 ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc
     5581 ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg
     5641 ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt taatcactat
     5701 accaattgag atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta
     5761 tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa
     5821 attccgctag acctttgtgt gttttttttg tttatattca agtggttata atttatagaa
     5881 taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac
     5941 tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa
     6001 acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga
     6061 atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag
     6121 ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta
     6181 tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg
     6241 cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg
     6301 ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc
     6361 taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtcaagagg
     6421 acatccggtc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttattcta
     6481 gagcacagct ccatcgaatg gccagatgat ccctatcagt gatagagaaa agtgaaatga
     6541 atagttcgac aaaaatctag acctgcaggt gcagtaagga ggaaaaaaaa atggacaaga
     6601 agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc attacggacg
     6661 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
     6721 agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc
     6781 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
     6841 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
     6901 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
     6961 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag
     7021 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat
     7081 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
     7141 tctttatcca actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat
     7201 ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcc
//
