LOCUS       sequence-214711-        2637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty SpecR p15A vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA
  TITLE     Engineered promoters enable constant gene expression at any copy 
            number in bacteria.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111.
  PUBMED    29553576
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111."
COMMENT     Sequence Label: pTHSSe_2
FEATURES             Location/Qualifiers
     source          1..2637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(411..956)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(491..510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1424..1442
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1446..1489)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(1662..2453)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPVESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
       61 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
      121 ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
      181 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
      241 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
      301 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
      361 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
      421 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      481 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
      541 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
      601 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      661 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
      721 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
      781 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
      841 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
      901 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
      961 atcatcttat taaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1261 gatacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattcca tggtgccacc
     1381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1441 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgaa
     1501 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc tcgagtcccg tcaagtcagc
     1621 gtaatgctct gccagtgtta caaccaatta accaattctg attatttgcc gactaccttg
     1681 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgtgcg cgaggccaag
     1741 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     1801 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     1861 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     1921 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     1981 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     2041 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     2101 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     2161 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     2221 acaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     2281 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     2341 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     2401 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct cataacaccc
     2461 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     2521 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     2581 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtg
//
