LOCUS       sequence-214798-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NRPS module containing CATR domains with C-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Schneider BA, EP Balskus
  TITLE     Discovery of small molecule protease inhibitors by investigating a 
            widespread human gut bacterial biosynthetic pathway,
  JOURNAL   Tetrahedron
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Tetrahedron"
COMMENT     Sequence Label: pET29b-RupA_C2-A2-T2-R-CHis
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(559..1374)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(559..1374)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1282..1301
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1366..1385
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1496..2084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1985..2004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2238..2255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2270..2409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2511..2702)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3511..4593)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4555..4574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4594..4671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4877..4896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5075..5119
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             9398..9415
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9468..9486)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9482..9529
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     3781 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatataca tatgaaagaa accgctgctg ctaaattcga
     5101 acgccagcac atggacagcc cagatctaat gttcagcggt acggcaaatc agaaaactgt
     5161 caaaaaagaa gaaaagaaag aacataaggc tctctatcct ctttcaaaga ctcagctcgg
     5221 cgtgtatatt gactgtatga aaaatccgta cagcacactt tacaatattc cgtcaattct
     5281 caaattttca aaatcagtcg atgcacaaaa gcttgccgac tgcgttgtaa aggtaatcaa
     5341 ggctcaccca tatattatga ctcacctttc acttgaaaac gatgatattg agcaggcata
     5401 tgttgactct gcaaaaccga atgtccccgt tgaaaagctt acggaagaac agcttgaatc
     5461 ctacaaaaag gattttgtaa aaccgcacaa tcttatgaaa tcaccgctgt tcagaatctc
     5521 ggttgcagaa accgaaaagg cggtatatct gctttcagat ttccaccacc ttatctttga
     5581 cggtgcgtcc gttgcacttt tttttgaaca gcttaaaacg ctgtacgaag ggggtaatat
     5641 tgagcctgaa agctacacct actttgatta tgcagagaat gaaattaagg ctgaaagcag
     5701 tgatgaattt agaaatgctg aaaagttctt cgacaatatg atgaaaagtt ttgaatctgc
     5761 aagcgaaatc acggctgacc tcagaggtca tgcagaggac ggtgctcttg catctcaggc
     5821 agtccccgtt gatatggcaa gggttgaaaa cttctgttca cagcacggaa tcactcccgc
     5881 ccaccttttc cttgccggca cattctacgc tgtttcaaga tttgtgaaca gcagaaatgt
     5941 ttacatcagc acaatcagca acggcagaag cgatatgcgg cttacaaact gctttggtat
     6001 gtttgtaaaa actcttgcgc tcggcattga aattgaggac atcacctcac ttgaatttgt
     6061 tgaaaaaagc aaagctgttt tcacggattc aattgaaaac gaaatttatc cctatgcaca
     6121 gctttgtgca aaatacggct atgcgccgaa cattatgtac gaatatcagc ttggtgttgt
     6181 tgataacctt gaaattgacg gcaaagccgt tgtccgtgat taccttgaaa tgaatactgc
     6241 aaaatttaaa acagccgttc acattgagga ttacaagggc aagccgagcg ttgtggttca
     6301 gtacaatgac gcactttaca gcggtgagct tatgagaacg cttgcaaaat ccgttctctg
     6361 cgctgttgaa catattatcg aaaatccaaa cggcaaaatc agaaaggttt cactccttga
     6421 caacgccgca attgctcagc ttgaaagctt taagtcaaca gaaattgctc ccgttaagac
     6481 aaaacttctt cacaaaatgt ttgaagaaca ggttgcaaaa actcccgaca gaattgctct
     6541 ttcggcttgt gacggtaaac taacctataa agagcttgac cgtcttgcaa atatcacggc
     6601 aaactcactc attgaaaaag gtcttgaaaa aggcggaaag gttcttattc tgcttgaaag
     6661 aacctcaaaa ttcttcatat cacttttcgg tattctcaag gccggcggtg cgtttattcc
     6721 gtcatgtccc gactatccga aggagcgaat cgacagtatt attgaggaca gtgacgcaga
     6781 ctttgtaatt acgaacggcg aactgctcaa taaatatgac cgcacggttg atgtttcaaa
     6841 acttctttca ggcaataatg aagaaaatcc gaatgttgat gtacagcctg acgaccttgc
     6901 gtaccttatc tacacctcag gttcaacagg taagccaaag ggcgttatgc tacgccacat
     6961 cggcattgca aactaccttg catacagcga ttcaaacatt caggttaaag aggttgttga
     7021 caactgtcac gcatacggct ctgtaactac aatcagcttt gatatgtcgc taaaggaaac
     7081 catgctgtcg ctctgcaacg gtcttacact cgtttttgca agtgaagaac agacggtaaa
     7141 cccgatgtca cttgcagaat tctttaaaga aaacaatgtt gatgcgttca actctacacc
     7201 gtcaagactt ttgcaatata tggagcttga cgaatttgca gaagcaatgg caaactgcaa
     7261 ggttattctc tcgggcggtg aaaaatatcc cgacaagcta ttaaggatac tgcgtgaaaa
     7321 gacgaacgca acaataatca acacctacgg tccgactgaa atcacggttt cttcaaacgc
     7381 aaaaaatctt acgaatgcag acgaaatttc aatcggcagg ccgttacgca actacacgga
     7441 atatatcgtt gactcagaca acaatctcct tccaatcgga gttgtgggcg agcttctcat
     7501 tcagggatgc ggtgtggcac tcggctacaa caaacttccc gaacagactg caaaggcatt
     7561 catcgaattt aacggtgaac gcacctaccg ctcgggcgac tacgcaaaat ggacaacaga
     7621 cggcgatgtt attattctcg gcagaaccga caatcaggtt aagctgagag gattaagaat
     7681 tgaactcggt gaaattgaaa agtgtctgac cgcagttgac ggaatcaaga gcggaattgc
     7741 tctcataaga aaggtcggca aggctgacgc aatctgcgtt tactatacag ccgacagaca
     7801 gattgagcca aatgaaatta agtctgagct tgcaaaagct ctcacggatt atatggttcc
     7861 gagcgcatat gttcagcttg acgaaatgcc tctcacacca aacggcaagg tcaacacaaa
     7921 ggttcttccc gaacctaaaa gcaacaaatc cgaaagaggt cggttgccga agaatgaact
     7981 tgaaaaaaca ttctgcgaca tctttgcaga aattcttgaa cttgacaatg tttttgcaga
     8041 cgataacttc tttgacctcg gcggaacatc gcttacagca acaagaattg taatctccgc
     8101 atcaaagaaa aatattgagg ttgcatacag cgacattttt gcaaatccta ctccgcagac
     8161 tcttgcaaaa tttgtgagca aggatgattc agccgaagac gaccttgaaa atctttcgga
     8221 ctacgattat acaaatatca ataaagttct tgaaaagaat aatattgata catttaaaaa
     8281 cggtgagctt caaaaactcg gcaatgttct tctgacaggc tctgccggct tcctcggagt
     8341 acacattctc tacgaacttc ttcacaagta taacggcaag gtttactgca tgattcgtga
     8401 caaaaacaat aatcctgccg aaaacagaat gaactcaatc tactattact actttgaaga
     8461 aagtctgaaa gaaagatatc ccgacagagt taccgttatc agcggtgatg ttacaaaccg
     8521 tgaatcattc gataaattta ttgacaagga cattaacacg gtcatcaact gtgccgcaaa
     8581 tgttaagcac ttttcaaagg gtacagacat tgaagatgta aacctttacg gtactctcaa
     8641 tgtgcttgac ttctgcaaaa aggctaatgc aaggcttgtt catgtgtcaa caatgagcgt
     8701 aggcggtatg tttgtgggcg aacaaggttc tgttgacaag ctcaaagaaa atcaacttta
     8761 ctttggtcag catgagggtt caaagtacac cctgtcaaag ttccttgcgg aaagggcgat
     8821 tcttgaagag gtttcaaagg gctttaacgc aaagattatg cgtgtaggca cacttgccgc
     8881 aagaaacagc gacggtgaat atcagattaa tttcaccacc aacacattta tgggcagact
     8941 caaatctaca ctccttatcg gcaaatatcc gtatgaggca atggaaatgc cgtttgagct
     9001 ttctccgatt gattttgtgg caaaggcaat tcttctgctt gcacaggctc cgaaggattg
     9061 caccgtgttc cacccgttca acaaccatac tctcattatg ggcgacctct acaccgagat
     9121 gaacaaaatc ggacttcatt cacagggtgc agagtatgag gaatatatga ttgcacttga
     9181 cagagctgag caggatcccg aaaaagcaaa gattctttcg agtatgattg catatcagaa
     9241 catggctcac ggtcaaaaga catttacagt cggcaagagc aacacatata caatgcaggt
     9301 tctttacaga atgggatttg tgtggcccgt tacctcgctt gactatatga aacgattcat
     9361 caacgcatta agaggtcttg gtttcttcga tctcgagcac caccaccacc accactgaga
     9421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     9481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     9541 aactatatcc ggat
//
