LOCUS       sequence-214822-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; notch promoter-driven expression of 
            tdTomato; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_CBF-tdTomato_L3-L2
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..87
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        292..488
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      339..474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      564..573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(703..721)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtataat aaagttgctg tggaatgtgt gtccatatga taaccgtatt
      121 accgccatgc attagttatt aatcgagatc tggtgtaaac acgccgtggg aaaaaattta
      181 tggatctgga gtaacacgcc gtgggaaaaa atttatggat ctggagtaac acgccgtggg
      241 aaaaaattta tggatctgga gtaacacgcc gtgggaaaaa atttatggat ctgcatctca
      301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
      421 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      481 tttgcaaaaa gctggctagc gctaccggac tcagatctcg agctcaagct tcgaattcgc
      541 caccatgcca gagccaggat cccgccacca tggtgagcaa gggcgaggag gtcatcaaag
      601 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
      661 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      721 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
      781 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
      841 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
      901 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
      961 cccccgacgg ccccgtaatg caraaaaaar accatggggc tgggaaggcc tccaccgaag
     1021 cgcctgtacc ccccccaacg gcttgctgaa agggcragat ccacccaggc cctgaagctg
     1081 aaaggacgg
//
