LOCUS       sequence-214823-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; notch promoter-driven expression of 
            tdTomato; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_CBF-tdTomato_L3-L2
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     488..507
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     682..705
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcaaaagctg gctagcgcta ccgggactca gatctcgagc tcaagcttcg aattcgccac
       61 catgccagag ccaggatccc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
      121 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      181 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      241 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      301 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      361 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      421 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
      481 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
      541 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
      601 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
      661 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
      721 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
      781 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
      841 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      901 tcgagggcga ggggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      961 accaaggngc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtaccg
     1021 ctccaaggcg tacgtgaagc accccgccrn c
//
