LOCUS       sequence-214825-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; notch promoter-driven expression of 
            tdTomato; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_CBF-tdTomato_L3-L2
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     115..131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     136..155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        136..154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    185..284
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     295..311
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     296..312
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(419..442)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(617..636)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     886..904
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
       61 tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa
      121 acagctatga ccatgtaata cgactcacta taggggatat cgcggccgcc ctgcagctgg
      181 atggcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa
      241 gcaatgcttt cttataatgc caactttgta caagaaagct gggtatctag aggctcgagg
      301 tcgacggtat cgataagctt gatatcgaat tcttacttgt acagctcgtc catgccgtac
      361 aggaacaggt ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg
      421 ttgtgggagg tgatgtccag cttggtgtcc acgtagtagt agccgggcag ttgcacgggc
      481 ttcttggcca tgtagatggt cttgaactcc accaggtagc ggccgccgtc cttcagcttc
      541 agggcctggt ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag
      601 gcctcccagc ccatggtctt cttctgcatt acggggccgt cgggggggaa gttggtgccg
      661 cgcatcttca ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg ggtcacggtc
      721 accagaccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      781 agcttcttgt aatcggggat gtcggcgggg tgcttcacgt acgccttgga gccgtacatg
      841 aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc
      901 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
      961 tggccgttca tggagccctc catgcgcacc ttgaagcgca tgaactcttt gatgacggcc
     1021 atgttgttgt cctcggaagg aggcggtggc cggagctgcc cgctgcccgg tgctgcccgg
     1081 tgccatgccc ccaga
//
