LOCUS       sequence-214839-        5299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NFIA1.1 protein for transient transfection assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Chaudhry AZ, Vitullo AD, Gronostajski RM
  TITLE     Nuclear factor I-mediated repression of the mouse mammary tumor 
            virus promoter is abrogated by the coactivators p300/CBP and SRC-1.
  JOURNAL   J Biol Chem. 1999 Mar 12;274(11):7072-81.
  PUBMED    10066764
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1999-03-12"; volume: "274(11)"; pages: "7072-81"
COMMENT     Sequence Label: pCMV-NFIA1.1
FEATURES             Location/Qualifiers
     source          1..5299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             834..860
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2438..2572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2462..2481)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2516..2535
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2683..2699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2683..2699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2696..2718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2707..2723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2731..2737,2738..2755,2756..2761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2776..2797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2914..2931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3085..3673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3165..3184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3844..4635,4636..4704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4467..4486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4705..4809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4877..4895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4933..4955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5055..5074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5268..5290
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5282..5299
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5283..5299
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgccagcc atgtatcctt
      841 acgatgtacc agactatgct gggcctcacg ggccgatgta ttctccgctc tgtctcaccc
      901 aggatgagtt tcatcctttc attgaagcac ttctgcccca tgtccgcgcc ttcgcctaca
      961 catggttcaa cctgcaggcc cgaaagcgga aatacttcaa aaaacatgag aagcgcatgt
     1021 cgaaagaaga ggagagggcc gtgaaggatg aactgctaag cgagaagccc gaggtcaagc
     1081 agaagtgggc ttcccgactt ctggccaagt tacggaaaga tatccgaccc gagtaccgag
     1141 aggattttgt tcttacagtt acagggaaaa aacctccatg ctgtgttctt tccaaccctg
     1201 atcagaaagg caagatgcgg agaattgact gcctccgcca ggcagataaa gtatggaggt
     1261 tggacctcgt catggtgatc ttgttcaaag gtattccgct ggaaagtact gatggcgagc
     1321 gccttgtaaa gagtccacag tgctctaatc cagggctctg tgtccagccc catcacatag
     1381 gggtttctgt aaaggaactc gatttatatt tggcatactt tgtacatgca gcagattcaa
     1441 gtcaatctga aagtcccagc cagccaagtg aagctgacat taaggaccag ccagaaaatg
     1501 gacatttggg cttccaggac agctttgtca catcaggtgt tttcagtgtg actgagctag
     1561 taagagtgtc acaaacacca atagctgcag gaaccggccc caatttttct ctctctgatt
     1621 tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct ctgcccagca
     1681 catcctctac cagctctaca aagcgcctca agtctgtgga ggatgaaatg gacagtcctg
     1741 gtgaagaacc attttacaca ggccaagggc gctccccagg gagtggcagc cagtccagtg
     1801 gatggcatga agtagagcca ggcttgccat ctccaagcac actgaagaag tctgagaagt
     1861 ctggtttcag cagcccctcc ccttcgcaga cctcctccct gggaacagca ttcacacagc
     1921 atcaccgacc tgtcattaca ggacccagag caagtccaca tgcgacgcca tcgactctcc
     1981 actttccaac gtcacccatc atccagcagc ctgggcctta cttctcacac ccagccatcc
     2041 gttaccaccc tcaggagacg ctgaaagagt ttgtccaact tgtctgtcct gatgctggtc
     2101 agcaagctgg acaggtgggg ttcctcaatc ccaatggaag cagtcaaggc aaggtgcaca
     2161 acccattcct ccccacccca atgttgccgc cgccgccacc accaccgatg gccaggcctg
     2221 tgcctctgcc catgccagac accaagcctc caaccacatc aacagaagga ggtgcagcct
     2281 cccccacctc accgacctac tcgacaccca gcacctcccc cgcaaaccga ttcgtcagtg
     2341 ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc tggtacctgg
     2401 gataaaagtg cagcatcccg ctagctaggg ccgcggggat ccagacatga taagatacat
     2461 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2521 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2581 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc
     2641 tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     2701 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2761 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2821 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2881 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2941 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3001 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4861 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     4921 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4981 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5041 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5101 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     5161 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5221 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5281 ttgtaaaacg acggccagt
//
