LOCUS       sequence-214858-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; hedgehog promoter-driven expression of 
            mTurquoise2; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(198..217)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             357..605
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQXKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     545..566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    788..808
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
       61 cctgkcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg
      121 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      181 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      241 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      301 tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca
      361 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
      421 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      481 ccgacaacca ctacctgagc acccagkcca agctgagcaa agaccccaac gagaagcgcg
      541 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      601 tgtacagtga gctgcagaac aagctggaag agttggatct ggactcgtac aagtaaagcg
      661 gccgcgactc tagatcataa tcagccatac cacatttgta gaggatccac tagtaacggc
      721 cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
      781 tagaggacaa ctttgtataa taaagttgaa cgagcaacgt agwatgatat waatatcaay
      841 atattatatt agattttgca taacaaacag acwacatatk actgtaaaac acarcatatc
      901 cagtcactat gaatcaacta
//
