LOCUS       sequence-214859-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; hedgehog promoter-driven expression of 
            mTurquoise2; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(541..562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(802..821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagcagcc accgcgggag cagcggcagc tgcggctgcc cgggcccggc agcgctgaga
       61 cccgcagggc accctcggac cccgcggcgg cggcggctct gcgcttgccc tccgcggccg
      121 ctcgggcgac ccgggaggcg ccgagagagc cagcgcggcc ggcgggagca gcggggattc
      181 gctggctctt tctgcagtga aggggtcgcg gcgcggggcg gggggcgggt agggggagtt
      241 ggggaccgca aggagtgccg cggaagcgcc cgaaggacag gctcgctcgg cgcgccggct
      301 ctcgctcttc cgcgaactgg atgtgggcag cggcggccgc agagacctcg ggacccccgc
      361 gcaatgtggc aatggaaggc gcagggtctg actccccggc agcggccgcg gccgcagcgg
      421 cagcagcgcc cgccgtgtga gcagcagcag cggctggtct gtcaaccgga gcggatctgc
      481 gatctaagta agcttatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      541 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg mgagggcgag
      601 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      661 gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt cgcccgctac
      721 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      781 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      841 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      901 aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta tatcaccgcc
      961 gacaagcaga agaacggcat caaggccaac ttcaagatcc gc
//
