LOCUS       sequence-214861-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; hedgehog promoter-driven expression of 
            mTurquoise2; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..77
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    299..422
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(477..496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(478..496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(501..517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(501..517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(619..638)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(697..716)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
       61 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacagtg
      121 agctgcagaa caagctggaa gagttggatc tggactcgta caagtaaagc ggccgcgact
      181 ctagatcata atcagccata ccacatttgt agaggatcca ctagtaacgg ccgccagtgt
      241 gctggaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagaggaca
      301 actttgtata ataaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
      361 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      421 tgaatcaact acttagatgg tattagtgac ctgtactgca gggcggccgc gatatcccct
      481 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      541 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      601 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      661 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      721 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
      781 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
      841 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
      901 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttana
//
