LOCUS       sequence-214885-        6929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Medium affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V
FEATURES             Location/Qualifiers
     source          1..6929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   215..344
                     /label=AAV2 ITR
     intron          937..1069
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1052..1071
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    1082..1115
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1178..1211
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1239..1385)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(1389..1418)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     1883..1904
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2007..2028)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     sig_peptide     complement(2997..3056)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    complement(3086..3119)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3190..3223)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3295..3883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3348..3368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3766..3777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3961..4082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3972..3991)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4026..4045
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4704..5292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4784..4803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5463..6254,6255..6323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6086..6105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6324..6428)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6454..6909)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6591..6612)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6803..6822
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct
       61 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
      121 gcctcagygg syrcagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
      181 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
      241 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      301 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      361 cccgccatgc tacttatcta cgtagccatg ctctaggaag atccgagctc cagtgtgctg
      421 gaattcgccc ttttcagtat ttaaattgac gaccgacccc gacccactgg acaagcaccc
      481 aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg
      541 gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg
      601 cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc
      661 gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc
      721 gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg
      781 cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc
      841 gcagctgtgc tcctgggcac cgcgcagtcc gcccccgcgg ctcctggcca gaccacccct
      901 aggaccccct gccccaagtc gcagccgttg gatcaggtaa gtatcaaggt tacaagacag
      961 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     1021 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgatgatccc
     1081 cataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
     1141 accatcgacc cgaattgcca agcatcacca tcgacacata acttcgtata atgtatgcta
     1201 tacgaagtta tactagtgct agcggatccg atatcctaac gtggcttctt ctgccaaagc
     1261 atgatgagga tgataaggga gatgatggtg agcaccacca gggccaggat ggctgagatc
     1321 accaccacct taaagggcaa ggagtgtggc accacgatga cctcctgcgt gtcctggccc
     1381 acagcattca gatcctcttc tgagatgagt ttttgttcgt cgacctgcag tttcagtgcc
     1441 ttgtcattcg gttctttgaa cagtgctttc atttcgtctg acagttcgaa attcatgttc
     1501 agggggttgt taaagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     1561 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     1621 cgggtcttgt aggtgccgtc gtccttgaag ctgatggtgc gctcctggac gtagccttcg
     1681 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
     1741 tgcacgccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     1801 gtgcagatga gcttcagggt cagcttgccg ttggtggcat cgccctcgcc ctcgccgcgc
     1861 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     1921 gtgaacagct cctcgccctt gctcatgctc cctccggtac cgcccttgta cagctcgtcc
     1981 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     2041 tcgttggggt ctttgctcag cacggactga tagctcaggt agtggttgtc gggcagcagc
     2101 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     2161 tcctccacgt tgtggcggat cttgaagttc gccttgatgc cgttcttctg cttgtcggcg
     2221 gtgatataga cgttgtggct caccagaatt ggatttttga accacttatc aaaccatttt
     2281 tccgcttcac cggaggtctg cacctgagcg atggtgtcat ccatcagctt tttgaactgc
     2341 ggatcatctt tacgcaacat acaaccgtag gcctcctgag actgcggctt gccgacgatt
     2401 tcccagttgt ctggtttctt cgctttcgca cgttcaccgg ccagcagcac gtcatccatc
     2461 ataaaggcaa cggcacgacc gctttccagg gtgcggaaag agtcaccgtg atctttggcg
     2521 ctgatgatgc gcatattcat tttttgctct tcattcagtt tgttgagcaa aacttcagag
     2581 gtagtgccgg aagtgacgac tacggctttg tctttcaggt tggcaaaatc tttgatatcg
     2641 ccaccctttt tggtcaacag gcgcgtaccg accacgaaaa tagtgtcaga gaaagccgcc
     2701 tgtttttggc gttcgacgtt gttggtggta gaaccacatt caaaatcgaa agtgccgttt
     2761 tgcagcagtg gaatacggtt ttgtgaggta atcggaatca gttttacctg caagtccggt
     2821 ttgttgagtt tctttttcac tgcttcaaca atggcgttgg agtaatcctg cgagtaaccc
     2881 accacttttt gctgattgtc gtaataagag aaaggcactg aagattcacg gtgaccgacg
     2941 acaatcacac cgtttttggc gattttgtcc agcgtgctgc ctgcggcaga tctgtcacca
     3001 gtggaacctg gaacccagag cagcagtacc catagcagga gtgtgtctgt ctccatgggg
     3061 cgatatcacc ggtggtacca ctagtataac ttcgtatagg atactttata cgaagttatc
     3121 attgggattc ttcctatttt gatccaagca tcaccatcga ccctctagtc cagatctcac
     3181 catcgaccca taacttcgta tagcatacat tatacgaagt tatgtccctc gaagaggttc
     3241 gaaacactga aaagggcgaa ttctgcagat gatccactcg agaagcttat cgataatcaa
     3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc
     3901 gggcggccgc tcgagcatgc atctagagtc gacccgggcg gccgcttcga gcagacatga
     3961 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     4021 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     4081 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     4141 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt cctagagcat
     4201 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     4261 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4321 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg rscrckccct ctctgcrssc
     4381 tcgctcgcyc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4441 ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga
     4501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6421 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     6481 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6541 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6601 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6661 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6721 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6781 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6841 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6901 agggcgcgtc ccattcgcca ttcaggctg
//
