LOCUS       sequence-214900-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  brr2-Q904E (endogenous promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Brr2 is a splicing fidelity factor
  JOURNAL   bioRxiv 2018. 354514
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2018. 354514"
COMMENT     Sequence Label: brr2-Q904E pRS313
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..57
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     291..308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    425..446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(461..466,467..484,485..491)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     504..526
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     523..539
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     523..539
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7677..7694)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7677..7693)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7686..7708)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8208..8500
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8501..8954
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(9644..10306)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10307..10494)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        10916..11020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11021..11089,11090..11881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11239..11258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12052..12503,1..137)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
       61 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      301 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      421 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      481 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
      541 tgattacgaa ttcgagctcc attttgttat ttgcgagccc aatgaactga gactatattg
      601 taataagggc cgtttgttca aattcgtact atccatatca ttcagacagg aacaaacttg
      661 ataattagtg gtgttgaatc aagtactatt aacgcattgt gatgatctct cagatatcaa
      721 tgcgtgtttg cttctttgac gatctaatat tgaatattgt cgctctttac gagaatatta
      781 aaaatttatg ctactaaagc tatgtctact ataaaggaat aatacactaa tagagataat
      841 cttgagtaaa ataaggtgaa ggtaaatata agtaattgct ttggaagatt taccgtgagc
      901 cttcgttatt aaaaatgact gagcatgaaa cgaaggataa ggccaaaaaa attagagaaa
      961 tttatcgtta tgatgagatg tccaataagg tgttgaaagt tgataaacgc tttatgaata
     1021 ccagccaaaa tcctcaaagg gatgcggaaa tatcacaacc aaaatccatg agtggtagaa
     1081 ttagtgctaa ggatatggga cagggcctgt gcaataacat taataaagga ctaaaagaaa
     1141 atgatgtagc cgttgaaaaa actggaaaga gcgcatcatt gaaaaaaatt cagcagcata
     1201 acaccatttt gaattcttcc tccgatttca ggttacatta ttatccaaag gatccctcaa
     1261 atgtcgaaac ttacgagcag attttgcagt gggttacaga agttttaggc aatgatatac
     1321 cccatgatct tattattgga actgctgata tattcatcag acaattgaaa gaaaatgagg
     1381 aaaatgagga tggtaatatt gaagagcgaa aggaaaagat ccagcatgag ctaggcatca
     1441 atatagactc cctgaagttt aacgaattag ttaaacttat gaaaaacatt accgattatg
     1501 aaacacatcc tgacaatagc aataaacaag cagtagcaat tcttgccgat gatgaaaaat
     1561 cagatgaaga agaagtaaca gaaatgtcca acaatgctaa tgttcttggg ggagaaatca
     1621 atgataatga agatgatgat gaggaatatg attacaatga cgttgaagtc aattctaaaa
     1681 aaaaaaacaa gagggcattg ccaaacattg aaaacgatat aattaaactc tccgatagta
     1741 aaacaagtaa tatcgagagc gttccgatat actcaatcga tgaatttttt ttgcagagaa
     1801 aacttcggtc agagcttggt tacaaggata cctctgtaat acaagacctg tcagaaaaaa
     1861 ttttgaatga tattgaaaca cttgagcata atccagtagc gttggagcag aagttggttg
     1921 atctgcttaa attcgagaat atatcactgg ctgaatttat tctcaagaat agatctacaa
     1981 ttttttgggg catacgactg gcgaagagca cagagaatga aataccgaac ttaatagaga
     2041 aaatggttgc aaaaggtttg aatgatcttg tggaacagta taaatttaga gaaactacac
     2101 actcaaagcg tgaactcgat tccggtgacg accagccaca gtcttcggaa gcaaaaagaa
     2161 caaaattcag taatccagca ataccgcccg tcatagattt ggaaaagatt aaattcgacg
     2221 aaagttctaa attaatgacc gtgacaaaag tatccctacc ggaaggatca ttcaaaaggg
     2281 tgaaaccaca atatgacgaa atacatattc cagctcctag caaacctgtt attgattatg
     2341 agttgaaaga aattacttct ctaccagatt ggtgtcagga ggcgtttcct tcatcggaaa
     2401 caacgtcctt aaaccctata caatcaaagg tttttcacgc agcatttgaa ggtgattcaa
     2461 acatgctaat atgtgcaccc actggttcag gtaagacgaa tatcgcgtta ttgacggtat
     2521 tgaaagcctt atcccatcat tataacccga aaactaagaa attaaacctt tctgcattta
     2581 aaattgttta tatagcaccc ttgaaagcct tagtacagga acaagtccga gaatttcaaa
     2641 ggaggttggc ttttctaggt ataaaagtcg ctgaattgac tggtgattct aggcttagta
     2701 gaaagcaaat tgacgagacg caggttttgg tgtctacgcc agagaaatgg gacataacta
     2761 ctagaaattc aaataatttg gccatcgtag aacttgttcg tttgttgatt atagatgaaa
     2821 tacatttgtt acatgatgac aggggaccag tattggaaag catcgtggct aggacatttt
     2881 gggcttccaa atatggccag gaatacccca gaataatagg cctttctgcc accttaccaa
     2941 attatgagga tgtcggtagg tttttaagag ttccgaagga aggattgttt tattttgact
     3001 cctcatttag accgtgtcct ctatctcaac agttttgtgg cattaaggaa cggaactcct
     3061 tgaaaaagct gaaggctatg aatgatgctt gttatgaaaa ggtattggaa tccatcaatg
     3121 aaggtaatca aataatcgtg tttgttcatt ctcgaaaaga aacatcacga acagcaacat
     3181 ggttgaaaaa taaatttgct gaagagaaca ttactcataa attaaccaaa aatgatgcag
     3241 gatcgaagca gattttaaag acagaagcag ccaacgtact cgatccaagt ctacgaaagt
     3301 tgattgaaag cggtataggg acgcatcacg cagggctgac cagaagtgac agatccttat
     3361 ctgaagattt atttgcagac gggttgttgc aagtattggt atgcactgcg acgttagcat
     3421 ggggtgttaa tctacctgct catacagtga ttataaaagg aacagatgtc tattcacctg
     3481 aaaaggggtc ctgggaacaa ctttctccac aagatgtact tcaaatgtta ggtagagcag
     3541 gcagaccgag atatgacaca tttggcgagg gtattataat aacagaccaa tcgaatgttc
     3601 aatattatct ctcggtttta aatgagcaat taccaataga atcgcaattt gtttcaaaat
     3661 tagttgacaa cttaaatgca gaagttgtcg cgggtaacat taagtgcagg aatgatgctg
     3721 tgaattggtt ggcgtatact tacctatatg tcagaatgtt agcttcccct atgctttaca
     3781 aagtacctga tatttcaagt gatgggcaat tgaaaaagtt tagagaaagc ttagtccatt
     3841 cggccctatg cattttaaaa gaacaagagt tggtgttata tgatgctgaa aatgatgtaa
     3901 ttgaggcgac cgatctagga aatatagcgt cgtcatttta cataaatcat gcttccatgg
     3961 atgtttacaa tagagagtta gatgaacata caactcagat tgatcttttt agaatattct
     4021 caatgtcgga agagtttaag tacgtatcgg tgagatatga agagaaaaga gaactaaaac
     4081 aacttctaga gaaagcaccc attcctatca gagaagatat tgatgatcct ttagcaaaag
     4141 tgaatgtact actgcagtct tatttttccc agctaaagtt cgaaggtttt gcacttaatt
     4201 ctgatatagt gtttattcac caaaatgctg gtaggttatt gcgtgctatg tttgaaatat
     4261 gcttgaagag aggatgggga catcccacac ggatgttgtt aaatctttgc aaatctgcca
     4321 caactaaaat gtggccaaca aactgtcctt taagacagtt taaaacatgc cctgtcgaag
     4381 tgattaaaag gctggaagcc tctacagtac cgtggggaga ttatctacag ctagaaacac
     4441 ctgccgaagt tggtcgtgca atcagatctg aaaaatatgg aaaacaggtt tacgatttat
     4501 tgaaaagatt tcccaaaatg tccgtaacat gcaacgctca gccaattacc cgtagcgtta
     4561 tgaggttcaa cattgaaata atagccgact ggatatggga tatgaatgta cacggttccc
     4621 ttgaaccttt tttgctcatg ctagaagaca ctgatggtga ttcaatactt tattatgacg
     4681 tactattcat cactccagat atagttggtc atgaatttac cttatccttt acttatgagt
     4741 taaagcagca caatcaaaat aacttaccac ctaatttctt ccttactttg atatcagaaa
     4801 attggtggca cagtgaattc gagattccag tttccttcaa tggatttaaa cttccgaaaa
     4861 aatttcctcc tcctactccc cttctggaga atattagtat ttcaacatca gagcttggca
     4921 atgatgattt ttccgaagta tttgaattta aaactttcaa caagattcag agccaggtct
     4981 tcgagtctct ctacaattcg aatgactcgg tctttgtagg ttcaggaaag ggcacaggta
     5041 aaacagctat ggctgaatta gcactgttaa atcattggag acagaataaa ggaagagcgg
     5101 tttatatcaa tccgtctggg gaaaaaattg attttttgct atctgattgg aataaaaggt
     5161 tctctcatct tgctggtggt aagataataa ataagctagg aaatgatcct tcactaaatt
     5221 taaaattatt agcaaaaagc catgttctct tggctacccc cgtgcaattt gagcttttgt
     5281 cacgtcgttg gagacaaagg aagaacatcc aaagtttaga attaatgata tatgatgacg
     5341 ctcatgagat tagccaaggt gtttacggtg ctgtgtatga aacgctaatt tctagaatga
     5401 tatttatagc aacccaactt gaaaagaaaa ttcgatttgt ttgcctttcg aactgtttgg
     5461 caaatgctcg tgattttggt gaatgggctg gtatgacgaa gtcaaacatc tacaactttt
     5521 ctccaagcga aagaattgag cctcttgaaa ttaatattca gtcatttaaa gatgttgagc
     5581 acatatcatt caatttttcg atgcttcaaa tggcatttga agcatctgct gctgcagcgg
     5641 gaaatcgtaa ttccagttcg gtgttcttac catctagaaa ggattgtatg gaagttgcgt
     5701 ctgcatttat gaagttttca aaagcaattg aatgggatat gttgaacgtt gaagaagagc
     5761 aaattgtacc atacattgaa aagttaacag atggtcatct aagagcacct ttaaaacacg
     5821 gtgttggtat tttgtataag gggatggcat caaatgatga aagaattgtg aaaaggttat
     5881 acgaatatgg tgcggtatcg gttttactca tttctaagga ttgttctgca ttcgcttgta
     5941 aaactgatga agtgattatt ttaggaacca acctttatga tggtgcagag cataaatata
     6001 tgccatatac cataaatgaa cttctagaaa tggtgggact agctagtggt aacgattcaa
     6061 tggcaggcaa ggttttaatt ttgacaagtc acaacatgaa ggcctactac aaaaaattct
     6121 taattgagcc tttgcctact gagagttacc tacagtatat tattcatgat actctgaaca
     6181 acgagatagc aaattctatt atacaaagca aacaagattg tgttgactgg tttacatatt
     6241 cgtatttcta tcgtcgtatc catgtgaatc caagctatta tggcgtaagg gatacgtctc
     6301 cacatggaat ttcagttttt ttgagtaatt tagttgaaac ttgtttgaat gacttagttg
     6361 aatcatcctt cattgaaatt gacgatacag aagctgaagt aaccgcagaa gttaatggag
     6421 gagatgatga agcaactgaa atcatttcta ccttgagcaa cgggctaatt gcttctcact
     6481 atggcgtatc attttttact attcagtcat tcgtttcttc tttatcgaat acgtcaacgc
     6541 tcaaaaacat gctgtatgtt ctttcaacag cagtagaatt cgaaagtgtt ccactcagaa
     6601 aaggagacag agcattactt gtcaaattaa gtaaaagact accattgaga ttccctgaac
     6661 acacctcttc tggttcggtg agctttaaag ttttcttatt gttacaagca tatttttcac
     6721 gtcttgaatt acctgtcgac tttcaaaatg atttaaaaga cattctagaa aaggttgttc
     6781 cactaattaa cgtagtggtt gatatccttt cagcaaacgg gtatttgaat gctactaccg
     6841 ccatggattt ggcgcagatg ttgattcagg gtgtatggga cgttgataat ccgttgagac
     6901 agattccaca cttcaacaac aaaattttgg aaaaatgtaa agaaattaac gtcgagacgg
     6961 tctatgatat aatggccctt gaggatgaag agagggatga aattttaacg cttactgatt
     7021 cacagcttgc gcaggtcgcc gcgtttgtta acaattaccc caacgttgaa cttacttatt
     7081 cgttgaataa ttctgattcg ttaatttcag gtgtaaaaca gaaaattacc attcaattga
     7141 caagagatgt tgaacctgag aacttacaag taacatctga aaagtatccg tttgataagc
     7201 tagaaagttg gtggttggtt ttaggtgaag tatctaaaaa agagctttat gcgatcaaga
     7261 aagttacttt aaataaagag actcagcaat atgaattgga atttgacact ccgacatctg
     7321 gtaaacataa cttaacaatt tggtgtgtct gtgattcata tcttgacgca gataaagagt
     7381 tgtcctttga aataaatgtg aaataaaatc attgtttagt cctggataat cgaatggatt
     7441 tcaatatata acattctctt gcggctattt aatctaattg tgatatatac agtttatagc
     7501 aaaagcgtat cattgcatca ctgcatttct atatcttcat cttcatcttc atcctgttct
     7561 ccttctttgt tttcgttttc aataaatcca ccttgatcct ctagagtcga cctgcagggg
     7621 tggagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg
     7681 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7741 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7801 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     7861 catctgtgcg gtatttcaca ccgcataact agcttttaac ttgtatccta ggttatctat
     7921 gctgtctcac catagagaat attacctatt tcagaatgta tgtccatgat tcgccgggta
     7981 aatacatata atacacaaat ctggcttaat aaagtctata atatatctca taaagaagtg
     8041 ctaaattggc tagtgctata tatttttaag aaaatttctt ttgactaagt ccatatcgac
     8101 tttgtaaaag ttcacattag catacatata ttacacgagc cagaaatagt aacttttgcc
     8161 taaatcacaa attgcaaaat ttaattgctt gcaaaaggtc acatgcttat aatcaacttt
     8221 tttaaaaatt taaaatactt ttttattttt tatttttaaa cataaatgaa ataatttatt
     8281 tattgtttat gattaccgaa acataaaacc tgctcaagaa aaagaaactg ttttgtcctt
     8341 ggaaaaaaag cactacctag gagcggccaa aatgccgagg ctttcatagc ttaaactctt
     8401 tacagaaaat aggcattata gatcagttcg agttttctta ttcttccttc cggttttatc
     8461 gtcacagttt tacagtaaat aagtatcacc tcttagagtt agcttacatt ttatgttagc
     8521 tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     8581 tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     8641 cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     8701 cacttgcctg caggcctttt gaaaagcaag cataaaagat ctaaacataa aatctgtaaa
     8761 ataacaagat gtaaagataa tgctaaatca tttggctttt tgattgattg tacaggaaaa
     8821 tatacatcgc agggggttga cttttaccat ttcaccgcaa tggaatcaaa cttgttgaag
     8881 agaatgttca caggcgcata cgctacaatg acccgattct tgctagcctt ttctcggtct
     8941 tgcaaacaac cgccggcagc ttagtatata aatacacatg tacatacctc tctccgtatc
     9001 ctcgtaatca ttttcttgta tttatcgtct tttcgctgta aaaactttat cacacttatc
     9061 tcaaatacac ttattaaccg cttttactat tatcttctac gctgacagta atatcaaaca
     9121 gtgacacata ttaaacacag tggtttcttt gcataaacac catcagcctc aagtcgtcaa
     9181 gtaaagattt cgtgttcatg cagatagata acaatctata tgttgataat tagcgttgcc
     9241 tcatcaatgc gagatccgtt taaccggacc ttttctttga ctacaacaca aaaatcaagc
     9301 acagaacgtc atacgaatcc gcaactacat tctgggcatt atgggctgga cttgccacga
     9361 aggagcaagc acagaaaatg gtggagaaag cactacccaa gttagagatg cttggaggtt
     9421 tagctgcatg tacggagcgt tctcgagttc aagagaaaaa aaaagaaaaa gcaaaaagaa
     9481 aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta ccttgtcatc
     9541 ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac atatatacac
     9601 atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata agaacacctt
     9661 tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg gcaaccgcaa
     9721 gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga caatcaacgt
     9781 ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc atctcgcaag
     9841 agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac ggcctgttcg
     9901 aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct gatccaaacc
     9961 tttttactcc acgcacggcc cctagggcct ctttaaaagc ttgaccgaga gcaatcccgc
    10021 agtcttcagt ggtgtgatgg tcgtctatgt gtaagtcacc aatgcactca acgattagcg
    10081 accagccgga atgcttggcc agagcatgta tcatatggtc cagaaaccct atacctgtgt
    10141 ggacgttaat cacttgcgat tgtgtggcct gttctgctac tgcttctgcc tctttttctg
    10201 ggaagatcga gtgctctatc gctaggggac caccctttaa agagatcgca atctgaatct
    10261 tggtttcatt tgtaatacgc tttactaggg ctttctgctc tgtcatcttt gccttcgttt
    10321 atcttgcctg ctcatttttt agtatattct tcgaagaaat cacattactt tatataatgt
    10381 ataattcatt atgtgataat gccaatcgct aagaaaaaaa aagagtcatc cgctaggtgg
    10441 aaaaaaaaaa atgaaaatca ttaccgaggc ataaaaaaat atagagtgta ctagaggagg
    10501 ccaagagtaa tagaaaaaga aaattgcggg aaaggactgt gttatgactt ccctgactaa
    10561 tgccgtgttc aaacgatacc tggcagtgac tcctagcgct caccaagctc ttaaaacgag
    10621 aattaagaaa aagtcgtcat ctttatacta agttggcatt ataaaaaagc attgcttatc
    10681 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gatttcaatt
    10741 ttgtcccact ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttatgc
    10801 ccgagaagat gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa
    10861 ctgcgcaact cgtgaaaggt aggcggatcc aggtggcact tttcggggaa atgtgcgcgg
    10921 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10981 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11041 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11101 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11161 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11221 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    11281 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11341 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11401 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11461 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11521 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11581 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11641 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11701 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11761 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11821 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11881 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12481 ggacaggtat ccggtaagcg gca
//
