LOCUS       sequence-214908-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Brr2 (endogenous promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Brr2 is a splicing fidelity factor
  JOURNAL   bioRxiv 2018. 354514
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2018. 354514"
COMMENT     Sequence Label: BRR2 pRS313
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    239..260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(275..280,281..298,299..305)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    313..329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     318..340
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     337..353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7491..7508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7491..7507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8022..8314
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8315..8768
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(9458..10120)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10121..10308)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        10730..10834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10835..10903,10904..11695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11053..11072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11866..12454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12355..12374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
       61 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      121 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
      361 cgaattcgag ctccattttg ttatttgcga gcccaatgaa ctgagactat attgtaataa
      421 gggccgtttg ttcaaattcg tactatccat atcattcaga caggaacaaa cttgataatt
      481 agtggtgttg aatcaagtac tattaacgca ttgtgatgat ctctcagata tcaatgcgtg
      541 tttgcttctt tgacgatcta atattgaata ttgtcgctct ttacgagaat attaaaaatt
      601 tatgctacta aagctatgtc tactataaag gaataataca ctaatagaga taatcttgag
      661 taaaataagg tgaaggtaaa tataagtaat tgctttggaa gatttaccgt gagccttcgt
      721 tattaaaaat gactgagcat gaaacgaagg ataaggccaa aaaaattaga gaaatttatc
      781 gttatgatga gatgtccaat aaggtgttga aagttgataa acgctttatg aataccagcc
      841 aaaatcctca aagggatgcg gaaatatcac aaccaaaatc catgagtggt agaattagtg
      901 ctaaggatat gggacagggc ctgtgcaata acattaataa aggactaaaa gaaaatgatg
      961 tagccgttga aaaaactgga aagagcgcat cattgaaaaa aattcagcag cataacacca
     1021 ttttgaattc ttcctccgat ttcaggttac attattatcc aaaggatccc tcaaatgtcg
     1081 aaacttacga gcagattttg cagtgggtta cagaagtttt aggcaatgat ataccccatg
     1141 atcttattat tggaactgct gatatattca tcagacaatt gaaagaaaat gaggaaaatg
     1201 aggatggtaa tattgaagag cgaaaggaaa agatccagca tgagctaggc atcaatatag
     1261 actccctgaa gtttaacgaa ttagttaaac ttatgaaaaa cattaccgat tatgaaacac
     1321 atcctgacaa tagcaataaa caagcagtag caattcttgc cgatgatgaa aaatcagatg
     1381 aagaagaagt aacagaaatg tccaacaatg ctaatgttct tgggggagaa atcaatgata
     1441 atgaagatga tgatgaggaa tatgattaca atgacgttga agtcaattct aaaaaaaaaa
     1501 acaagagggc attgccaaac attgaaaacg atataattaa actctccgat agtaaaacaa
     1561 gtaatatcga gagcgttccg atatactcaa tcgatgaatt ttttttgcag agaaaacttc
     1621 ggtcagagct tggttacaag gatacctctg taatacaaga cctgtcagaa aaaattttga
     1681 atgatattga aacacttgag cataatccag tagcgttgga gcagaagttg gttgatctgc
     1741 ttaaattcga gaatatatca ctggctgaat ttattctcaa gaatagatct acaatttttt
     1801 ggggcatacg actggcgaag agcacagaga atgaaatacc gaacttaata gagaaaatgg
     1861 ttgcaaaagg tttgaatgat cttgtggaac agtataaatt tagagaaact acacactcaa
     1921 agcgtgaact cgattccggt gacgaccagc cacagtcttc ggaagcaaaa agaacaaaat
     1981 tcagtaatcc agcaataccg cccgtcatag atttggaaaa gattaaattc gacgaaagtt
     2041 ctaaattaat gaccgtgaca aaagtatccc taccggaagg atcattcaaa agggtgaaac
     2101 cacaatatga cgaaatacat attccagctc ctagcaaacc tgttattgat tatgagttga
     2161 aagaaattac ttctctacca gattggtgtc aggaggcgtt tccttcatcg gaaacaacgt
     2221 ccttaaaccc tatacaatca aaggtttttc acgcagcatt tgaaggtgat tcaaacatgc
     2281 taatatgtgc acccactggt tcaggtaaga cgaatatcgc gttattgacg gtattgaaag
     2341 ccttatccca tcattataac ccgaaaacta agaaattaaa cctttctgca tttaaaattg
     2401 tttatatagc acccttgaaa gccttagtac aggaacaagt ccgagaattt caaaggaggt
     2461 tggcttttct aggtataaaa gtcgctgaat tgactggtga ttctaggctt agtagaaagc
     2521 aaattgacga gacgcaggtt ttggtgtcta cgccagagaa atgggacata actactagaa
     2581 attcaaataa tttggccatc gtagaacttg ttcgtttgtt gattatagat gaaatacatt
     2641 tgttacatga tgacagggga ccagtattgg aaagcatcgt ggctaggaca ttttgggctt
     2701 ccaaatatgg ccaggaatac cccagaataa taggcctttc tgccacctta ccaaattatg
     2761 aggatgtcgg taggttttta agagttccga aggaaggatt gttttatttt gactcctcat
     2821 ttagaccgtg tcctctatct caacagtttt gtggcattaa ggaacggaac tccttgaaaa
     2881 agctgaaggc tatgaatgat gcttgttatg aaaaggtatt ggaatccatc aatgaaggta
     2941 atcaaataat cgtgtttgtt cattctcgaa aagaaacatc acgaacagca acatggttga
     3001 aaaataaatt tgctgaagag aacattactc ataaattaac caaaaatgat gcaggatcga
     3061 agcagatttt aaagacagaa gcagccaacg tactcgatcc aagtctacga aagttgattg
     3121 aaagcggtat agggacgcat cacgcagggc tgaccagaag tgacagatcc ttatctgaag
     3181 atttatttgc agacgggttg ttgcaagtat tggtatgcac tgcgacgtta gcatggggtg
     3241 ttaatctacc tgctcataca gtgattataa aaggaacaga tgtctattca cctgaaaagg
     3301 ggtcctggga acaactttct ccacaagatg tacttcaaat gttaggtaga gcaggcagac
     3361 cgagatatga cacatttggc gagggtatta taataacaga ccaatcgaat gttcaatatt
     3421 atctctcggt tttaaatcaa caattaccaa tagaatcgca atttgtttca aaattagttg
     3481 acaacttaaa tgcagaagtt gtcgcgggta acattaagtg caggaatgat gctgtgaatt
     3541 ggttggcgta tacttaccta tatgtcagaa tgttagcttc ccctatgctt tacaaagtac
     3601 ctgatatttc aagtgatggg caattgaaaa agtttagaga aagcttagtc cattcggccc
     3661 tatgcatttt aaaagaacaa gagttggtgt tatatgatgc tgaaaatgat gtaattgagg
     3721 cgaccgatct aggaaatata gcgtcgtcat tttacataaa tcatgcttcc atggatgttt
     3781 acaatagaga gttagatgaa catacaactc agattgatct ttttagaata ttctcaatgt
     3841 cggaagagtt taagtacgta tcggtgagat atgaagagaa aagagaacta aaacaacttc
     3901 tagagaaagc acccattcct atcagagaag atattgatga tcctttagca aaagtgaatg
     3961 tactactgca gtcttatttt tcccagctaa agttcgaagg ttttgcactt aattctgata
     4021 tagtgtttat tcaccaaaat gctggtaggt tattgcgtgc tatgtttgaa atatgcttga
     4081 agagaggatg gggacatccc acacggatgt tgttaaatct ttgcaaatct gccacaacta
     4141 aaatgtggcc aacaaactgt cctttaagac agtttaaaac atgccctgtc gaagtgatta
     4201 aaaggctgga agcctctaca gtaccgtggg gagattatct acagctagaa acacctgccg
     4261 aagttggtcg tgcaatcaga tctgaaaaat atggaaaaca ggtttacgat ttattgaaaa
     4321 gatttcccaa aatgtccgta acatgcaacg ctcagccaat tacccgtagc gttatgaggt
     4381 tcaacattga aataatagcc gactggatat gggatatgaa tgtacacggt tcccttgaac
     4441 cttttttgct catgctagaa gacactgatg gtgattcaat actttattat gacgtactat
     4501 tcatcactcc agatatagtt ggtcatgaat ttaccttatc ctttacttat gagttaaagc
     4561 agcacaatca aaataactta ccacctaatt tcttccttac tttgatatca gaaaattggt
     4621 ggcacagtga attcgagatt ccagtttcct tcaatggatt taaacttccg aaaaaatttc
     4681 ctcctcctac tccccttctg gagaatatta gtatttcaac atcagagctt ggcaatgatg
     4741 atttttccga agtatttgaa tttaaaactt tcaacaagat tcagagccag gtcttcgagt
     4801 ctctctacaa ttcgaatgac tcggtctttg taggttcagg aaagggcaca ggtaaaacag
     4861 ctatggctga attagcactg ttaaatcatt ggagacagaa taaaggaaga gcggtttata
     4921 tcaatccgtc tggggaaaaa attgattttt tgctatctga ttggaataaa aggttctctc
     4981 atcttgctgg tggtaagata ataaataagc taggaaatga tccttcacta aatttaaaat
     5041 tattagcaaa aagccatgtt ctcttggcta cccccgtgca atttgagctt ttgtcacgtc
     5101 gttggagaca aaggaagaac atccaaagtt tagaattaat gatatatgat gacgctcatg
     5161 agattagcca aggtgtttac ggtgctgtgt atgaaacgct aatttctaga atgatattta
     5221 tagcaaccca acttgaaaag aaaattcgat ttgtttgcct ttcgaactgt ttggcaaatg
     5281 ctcgtgattt tggtgaatgg gctggtatga cgaagtcaaa catctacaac ttttctccaa
     5341 gcgaaagaat tgagcctctt gaaattaata ttcagtcatt taaagatgtt gagcacatat
     5401 cattcaattt ttcgatgctt caaatggcat ttgaagcatc tgctgctgca gcgggaaatc
     5461 gtaattccag ttcggtgttc ttaccatcta gaaaggattg tatggaagtt gcgtctgcat
     5521 ttatgaagtt ttcaaaagca attgaatggg atatgttgaa cgttgaagaa gagcaaattg
     5581 taccatacat tgaaaagtta acagatggtc atctaagagc acctttaaaa cacggtgttg
     5641 gtattttgta taaggggatg gcatcaaatg atgaaagaat tgtgaaaagg ttatacgaat
     5701 atggtgcggt atcggtttta ctcatttcta aggattgttc tgcattcgct tgtaaaactg
     5761 atgaagtgat tattttagga accaaccttt atgatggtgc agagcataaa tatatgccat
     5821 ataccataaa tgaacttcta gaaatggtgg gactagctag tggtaacgat tcaatggcag
     5881 gcaaggtttt aattttgaca agtcacaaca tgaaggccta ctacaaaaaa ttcttaattg
     5941 agcctttgcc tactgagagt tacctacagt atattattca tgatactctg aacaacgaga
     6001 tagcaaattc tattatacaa agcaaacaag attgtgttga ctggtttaca tattcgtatt
     6061 tctatcgtcg tatccatgtg aatccaagct attatggcgt aagggatacg tctccacatg
     6121 gaatttcagt ttttttgagt aatttagttg aaacttgttt gaatgactta gttgaatcat
     6181 ccttcattga aattgacgat acagaagctg aagtaaccgc agaagttaat ggaggagatg
     6241 atgaagcaac tgaaatcatt tctaccttga gcaacgggct aattgcttct cactatggcg
     6301 tatcattttt tactattcag tcattcgttt cttctttatc gaatacgtca acgctcaaaa
     6361 acatgctgta tgttctttca acagcagtag aattcgaaag tgttccactc agaaaaggag
     6421 acagagcatt acttgtcaaa ttaagtaaaa gactaccatt gagattccct gaacacacct
     6481 cttctggttc ggtgagcttt aaagttttct tattgttaca agcatatttt tcacgtcttg
     6541 aattacctgt cgactttcaa aatgatttaa aagacattct agaaaaggtt gttccactaa
     6601 ttaacgtagt ggttgatatc ctttcagcaa acgggtattt gaatgctact accgccatgg
     6661 atttggcgca gatgttgatt cagggtgtat gggacgttga taatccgttg agacagattc
     6721 cacacttcaa caacaaaatt ttggaaaaat gtaaagaaat taacgtcgag acggtctatg
     6781 atataatggc ccttgaggat gaagagaggg atgaaatttt aacgcttact gattcacagc
     6841 ttgcgcaggt cgccgcgttt gttaacaatt accccaacgt tgaacttact tattcgttga
     6901 ataattctga ttcgttaatt tcaggtgtaa aacagaaaat taccattcaa ttgacaagag
     6961 atgttgaacc tgagaactta caagtaacat ctgaaaagta tccgtttgat aagctagaaa
     7021 gttggtggtt ggttttaggt gaagtatcta aaaaagagct ttatgcgatc aagaaagtta
     7081 ctttaaataa agagactcag caatatgaat tggaatttga cactccgaca tctggtaaac
     7141 ataacttaac aatttggtgt gtctgtgatt catatcttga cgcagataaa gagttgtcct
     7201 ttgaaataaa tgtgaaataa aatcattgtt tagtcctgga taatcgaatg gatttcaata
     7261 tataacattc tcttgcggct atttaatcta attgtgatat atacagttta tagcaaaagc
     7321 gtatcattgc atcactgcat ttctatatct tcatcttcat cttcatcctg ttctccttct
     7381 ttgttttcgt tttcaataaa tccaccttga tcctctagag tcgacctgca ggggtggagc
     7441 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc
     7501 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7561 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7621 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     7681 tgcggtattt cacaccgcat aactagcttt taacttgtat cctaggttat ctatgctgtc
     7741 tcaccataga gaatattacc tatttcagaa tgtatgtcca tgattcgccg ggtaaataca
     7801 tataatacac aaatctggct taataaagtc tataatatat ctcataaaga agtgctaaat
     7861 tggctagtgc tatatatttt taagaaaatt tcttttgact aagtccatat cgactttgta
     7921 aaagttcaca ttagcataca tatattacac gagccagaaa tagtaacttt tgcctaaatc
     7981 acaaattgca aaatttaatt gcttgcaaaa ggtcacatgc ttataatcaa cttttttaaa
     8041 aatttaaaat acttttttat tttttatttt taaacataaa tgaaataatt tatttattgt
     8101 ttatgattac cgaaacataa aacctgctca agaaaaagaa actgttttgt ccttggaaaa
     8161 aaagcactac ctaggagcgg ccaaaatgcc gaggctttca tagcttaaac tctttacaga
     8221 aaataggcat tatagatcag ttcgagtttt cttattcttc cttccggttt tatcgtcaca
     8281 gttttacagt aaataagtat cacctcttag agttagctta cattttatgt tagctggtgg
     8341 actgacgcca gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt
     8401 aagcggaggt gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa
     8461 aaatgctaag aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg
     8521 cctgcaggcc ttttgaaaag caagcataaa agatctaaac ataaaatctg taaaataaca
     8581 agatgtaaag ataatgctaa atcatttggc tttttgattg attgtacagg aaaatataca
     8641 tcgcaggggg ttgactttta ccatttcacc gcaatggaat caaacttgtt gaagagaatg
     8701 ttcacaggcg catacgctac aatgacccga ttcttgctag ccttttctcg gtcttgcaaa
     8761 caaccgccgg cagcttagta tataaataca catgtacata cctctctccg tatcctcgta
     8821 atcattttct tgtatttatc gtcttttcgc tgtaaaaact ttatcacact tatctcaaat
     8881 acacttatta accgctttta ctattatctt ctacgctgac agtaatatca aacagtgaca
     8941 catattaaac acagtggttt ctttgcataa acaccatcag cctcaagtcg tcaagtaaag
     9001 atttcgtgtt catgcagata gataacaatc tatatgttga taattagcgt tgcctcatca
     9061 atgcgagatc cgtttaaccg gaccttttct ttgactacaa cacaaaaatc aagcacagaa
     9121 cgtcatacga atccgcaact acattctggg cattatgggc tggacttgcc acgaaggagc
     9181 aagcacagaa aatggtggag aaagcactac ccaagttaga gatgcttgga ggtttagctg
     9241 catgtacgga gcgttctcga gttcaagaga aaaaaaaaga aaaagcaaaa agaaaaaagg
     9301 aaagcgcgcc tcgttcagaa tgacacgtat agaatgatgc attaccttgt catcttcagt
     9361 atcatactgt tcgtatacat acttactgac attcataggt atacatatat acacatgtat
     9421 atatatcgta tgctgcagct ttaaataatc ggtgtcacta cataagaaca cctttggtgg
     9481 agggaacatc gttggtacca ttgggcgagg tggcttctct tatggcaacc gcaagagcct
     9541 tgaacgcact ctcactacgg tgatgatcat tcttgcctcg cagacaatca acgtggaggg
     9601 taattctgct agcctctgca aagctttcaa gaaaatgcgg gatcatctcg caagagagat
     9661 ctcctacttt ctccctttgc aaaccaagtt cgacaactgc gtacggcctg ttcgaaagat
     9721 ctaccaccgc tctggaaagt gcctcatcca aaggcgcaaa tcctgatcca aaccttttta
     9781 ctccacgcac ggcccctagg gcctctttaa aagcttgacc gagagcaatc ccgcagtctt
     9841 cagtggtgtg atggtcgtct atgtgtaagt caccaatgca ctcaacgatt agcgaccagc
     9901 cggaatgctt ggccagagca tgtatcatat ggtccagaaa ccctatacct gtgtggacgt
     9961 taatcacttg cgattgtgtg gcctgttctg ctactgcttc tgcctctttt tctgggaaga
    10021 tcgagtgctc tatcgctagg ggaccaccct ttaaagagat cgcaatctga atcttggttt
    10081 catttgtaat acgctttact agggctttct gctctgtcat ctttgccttc gtttatcttg
    10141 cctgctcatt ttttagtata ttcttcgaag aaatcacatt actttatata atgtataatt
    10201 cattatgtga taatgccaat cgctaagaaa aaaaaagagt catccgctag gtggaaaaaa
    10261 aaaaatgaaa atcattaccg aggcataaaa aaatatagag tgtactagag gaggccaaga
    10321 gtaatagaaa aagaaaattg cgggaaagga ctgtgttatg acttccctga ctaatgccgt
    10381 gttcaaacga tacctggcag tgactcctag cgctcaccaa gctcttaaaa cgagaattaa
    10441 gaaaaagtcg tcatctttat actaagttgg cattataaaa aagcattgct tatcaatttg
    10501 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgatttc aattttgtcc
    10561 cactccctgc ctctgtcatc acgatactgt gatgccatgg tgtccgactt atgcccgaga
    10621 agatgttgag caaacttatc gcttatctgc ttctcataga gtcttgcaga caaactgcgc
    10681 aactcgtgaa aggtaggcgg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10741 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10801 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10861 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10921 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10981 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11041 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    11101 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11161 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11221 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11281 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11341 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11401 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11461 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11521 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    11581 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    11641 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    11701 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11761 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12001 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12481 gttcctggcc ttttgctggc ctt
//
