LOCUS       sequence-214931-       10628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding codon-optimized yellow
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_yECitrine
FEATURES             Location/Qualifiers
     source          1..10628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3875..3908)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3917..4633
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      4654..4841
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4709..4733)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4916..6272
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4916..5259
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5260..6069
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5327..5346)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6075..6272
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6366..6383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6366..6382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6375..6397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9277..10371
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatgcgcgg ccgcataact tcgtatagca tacattatac
     3901 gaagttatgc atgctcatgt ctaaaggtga agaattattc actggtgttg tcccaatttt
     3961 ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaagg
     4021 tgatgctact tacggtaaat tgaccttaaa atttatttgt actactggta aattgccagt
     4081 tccatggcca accttagtca ctactttagg ttatggtttg atgtgttttg ctagataccc
     4141 agatcatatg aaacaacatg actttttcaa gtctgccatg ccagaaggtt atgttcaaga
     4201 aagaactatt tttttcaaag atgacggtaa ctacaagacc agagctgaag tcaagtttga
     4261 aggtgatacc ttagttaata gaatcgaatt aaaaggtatt gattttaaag aagatggtaa
     4321 cattttaggt cacaaattgg aatacaacta taactctcac aatgtttaca tcatggctga
     4381 caaacaaaag aatggtatca aagttaactt caaaattaga cacaacattg aagatggttc
     4441 tgttcaatta gctgaccatt atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt
     4501 accagacaac cattacttat cctatcaatc tgccttatcc aaagatccaa acgaaaagag
     4561 agaccacatg gtcttgttag aatttgttac tgctgctggt attacccatg gtattgatga
     4621 attgtacaaa taaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
     4681 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     4741 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     4801 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
     4861 agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     4921 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     4981 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     5041 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     5101 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     5161 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     5221 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac
     5281 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     5341 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     5401 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     5461 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     5521 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
     5581 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     5641 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     5701 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     5761 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     5821 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     5881 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     5941 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     6001 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     6061 tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     6121 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     6181 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     6241 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     6301 cagtcgacga actagtataa cttcgtatat ggtattatat acgaagttat gcggccgctg
     6361 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     6541 ctccttacgc atctgtgcgg tatttcacac cgcataccat cgtccggatc aatgaagtag
     6601 atgagtttac tacctgtata tacttttttt tttgtcaaag tcacttttat ggcatttcca
     6661 tgttgaagga ttagccacag cactaaattt tcagcgtatg attgctttta aatatttaat
     6721 tttcatcgtt ttttaattaa tattcaaaac gatttaatgc cttctacatc tgaagtcaag
     6781 ctcatagact cgggttgggt agctaaaact acatctaaca cgaaggggta tagccttctt
     6841 aaagtatact catgtataaa cgttttgggt tcatattttt gggccacgtg gtttataaaa
     6901 attcttaact acaccactcg gtatcccgca cccgtctacg tttctacgat ttcgagctaa
     6961 tattgagtaa agtgattagc aaaaaaaata aaagtactcg ttgtcggaga tcaagaattt
     7021 ttctattatc tcatctaaac cactttctaa aagcgaaaaa caaaatcgta atatgcagct
     7081 tgaatgggct tccatagttt gaaagaaaaa ccctagcagt actggcaagg gagacattcc
     7141 ttttacctgg ctaatcgcca tcttccagca ggcgcaccat tgcccctgtt tcactatcca
     7201 ggttacggat atagttcatg acaatattta cattggtcca gccaccagct tgcatgatct
     7261 ccggtattga aactccagcg cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc
     7321 cagaccaggc caggtatctc tgaccagagt catccttagc gccgtaaatc aatcgatgag
     7381 ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg gctggtggca gatggcgcgg
     7441 caacaccatt ttttctgacc cggcaaaaca ggtagttatt cggatcatca gctacaccag
     7501 agacggaaat ccatcgctcg accagtttag ttacccccag gctaagtgcc ttctctacac
     7561 ctgcggtgct aaccagcgtt ttcgttctgc caatatggat taacattctc ccaccgtcag
     7621 tacgtgagat atctttaacc ctgatcctgg caatttcggc tatacgtaac agggtgttat
     7681 aagcaatccc cagaaatgcc agattacgta tatcctggca gcgatcgcta ttttccatga
     7741 gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac
     7801 cggcatcaac gttttctttt cggatccgcc gcataaccag tgaaacagca ttgctgtcac
     7861 ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga
     7921 tagtttttac tgccagaccg cgcgcctgaa gatatagaag ataatcgcga acatcttcag
     7981 gttctgcggg aaaccatttc cggttattca acttgcacca tgccgcccac gaccggcaaa
     8041 cggacagaag cattttccag gtatgctcag aaaacgcctg gcgatccctg aacatgtcca
     8101 tcaggttctt gcgaacctca tcactcgttg catcgaccgg taacttttag attaacattt
     8161 gtttaaatat gaaattaggc aatgtaacat actgcaggca aattttggtg tacggtaagt
     8221 aaattggaca tatgatttaa caaagcgact ataagtcaga aagtgagaat gagattgaaa
     8281 taattaattc aacttattca attgatgata tgccaggatt cctcttcctt tatcttcaat
     8341 gtttcgatta acaggtccat tatccggttt ttagtttcgc tgcacattgc cgaatgacaa
     8401 tcggattcct ttaagctttg cttttgccct gttttctctc cccagtaatc gttgggtgac
     8461 tgaaccatcc agtagccgag agttacgttt ttcctgattt aataaatatc catatctatt
     8521 tgtctcgtgg tgttttacag caactaaccg aaattactat tttgcatcat ccaaccataa
     8581 agagacaaca ataattctag aaaacatata acaagtgttt ttttctattt acgtctcata
     8641 cgcaaagtcc actttaccat gttttggaaa cttgtaccca accattcatt tttcgatttt
     8701 gtaatgaggc tcttttcttc taacaatcat tgaggtcatt atcatgtgtc ctcacctatt
     8761 gtttaaattg aggcttgcgc tattcccaaa attgtgggta ttattttcat atatcagtat
     8821 ctgcaatttc ggttcaactc cggaagctcg gtacccaact gtgggaatac tcaggtatcg
     8881 taagatgcaa gagttcgaat ctcttagcaa ccattatttt ttttctctca acataacgag
     8941 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
     9001 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
     9061 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     9121 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     9181 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     9241 atatatatat atatatttca aggatatacc attgtaatgt ctgcccctaa gaagatcgtc
     9301 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
     9361 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
     9421 gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag
     9481 gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
     9541 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
     9601 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
     9661 ggtactgact tcgttgttgt tagagaatta gtgggaggta tttactttgg taagagaaag
     9721 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
     9781 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
     9841 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
     9901 accatcaaga acgaatttcc tacattgaaa gttcaacatc aattgattga ttctgccgcc
     9961 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
    10021 ggtgatatca tctccgatga agcctccgtt atcccaggct ccttgggttt gttgccatct
    10081 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccat
    10141 ggttccgctc cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct
    10201 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaagccat tgaagatgca
    10261 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
    10321 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
    10381 ctttttttgt gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
    10441 aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
    10501 tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa
    10561 tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg
    10621 gcatcaca
//
