LOCUS       sequence-214932-        8835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8835)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W3...
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(244..263)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     513..531
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2153..2162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2315..2618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2619..2822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2773..2793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2884..2893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2890..3591
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3592..3657
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3682..3738
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4984..5008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5035..5607
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    5804..5859
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6334..6352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6420..6524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6525..6593,6594..7385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6743..6762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7556..8144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8045..8064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8298..8315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8713..8768
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
       61 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
      121 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
      181 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
      241 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
      301 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
      361 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
      421 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
      481 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
      541 tgccctcgta ggggcggccc tgaggaaaaa aaaggaaagg gcatgctcgc catgtgcaag
      601 atacagtgtt gaatgtggtg caaaatataa agatgtggaa gaaaaagcca ctgaaaataa
      661 tatgaggaaa taaaaaacta gagtaaatta tcagtggaac aatttctatt atacaagaca
      721 ctacacccat acattctcct aatgctcatg taagaatttc tccaaaaata ccttccagca
      781 ctacaaacta gaaaaaaaaa gagacatgga caccaaatta agttcttaat agtgaagaac
      841 aaaagaacta ccttgcctgc tccagtggat ccagcaaccg ccaacaactg tcctctttct
      901 atcttgaaat taatatcttt caggacagga gtaccaagaa gtgagaaatt actgaagaag
      961 aggctgtcat caccattaga agtttttcta ttgttattgt tttgttttgc tttctcaaat
     1021 tattccccaa atccctgtta aaaaaaaaca cacacacaca cacacacaca cacatcaaaa
     1081 ataaaagatg agtttgtcag atattttcaa tagatgcttg ttttatgtac catactcacc
     1141 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
     1201 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct ggttccggtg
     1261 cctccacttt catcttcacc tttagtgata ccggctgccg tcacgaactc cttcaggacc
     1321 atgtgatcgc gcttctcgtt cgggtctttg ctcagcgtgg tctgggtgct cagatagtgg
     1381 ttgtccggca ggcgcaccgg gccgtcgccg atcggggtgt tctgctgttt gtggtcggct
     1441 tcctgctggc tgccgtcctc aacgttgtgg cggatcttga agttagcctt gatgccgttc
     1501 ttctgcttgt cggcttcgat acggacgttg tggctgttgt ggttgtactc cagcttgtga
     1561 cccaggatgt tgccgtcctc cttgaagtcg ttgcccttca gctcgatgcg gttcaccagg
     1621 gtgtcgccct cgaacttcac ctccgcgcgg gtcttgtagg tgccgtcgtc cttgaactcg
     1681 atggtgcgct cctggacgta gccttccggc atggcggact tgaagaagtc gtgacgcttc
     1741 atgtggtccg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtc
     1801 ggccacggca ccggcagctt gccggtggtg cagatgaact tcagggtcag cttgccctcg
     1861 gtggcatcgc cctcgccctc gccacgcacg ctgaacttgt ggccgtttac gtcgccgtcc
     1921 agctcgactt tgataggcac agtacctgta aatagttcct ctcctttact ggtaccatca
     1981 tcagggaatg agcttacatt tctcctcttc agcatctggt catccttctt agctttcctc
     2041 agctccacat tgacctctat tctgcgacgc ctcatttctg tactgtcttt tcccttgttc
     2101 ttgaatctgt gaagacgggc agctggtgta ttagcattct cgttggtgga tcccatggtg
     2161 gcaccggtga attctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc
     2221 ctcccaccgt acacgccacc tcgacatact cgagtagtta ttaatagtaa tcaattacgg
     2281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2821 ctggtttagt gaaccgtcag atccgctagg gatcctctag tcagctgacg cgttaccacc
     2881 ggtgccacca tgggaagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     2941 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     3001 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     3061 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     3121 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     3181 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     3241 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     3301 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     3361 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     3421 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     3481 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     3541 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc
     3601 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     3661 ggatctagag gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     3721 gaggagaacc ctggacctat gtctgaatat attcgggtaa ccgaagatga gaacgatgag
     3781 cccattgaaa taccatcgga agacgatggg acggtgctgc tctccacggt tacagcccag
     3841 tttccagggg cgtgtgggct tcgctacagg aatccagtgt ctcagtgtat gagaggtgtc
     3901 cggctggtag aaggaattct gcatgcccca gatgctggct ggggaaatct ggtgtatgtt
     3961 gtcaactatc caaaagataa caaaagaaaa atggatgaga cagatgcttc atcagcagtg
     4021 aaagtgaaaa gagcagtcca gaaaacatcc gatttaatag tgttgggtct cccatggaaa
     4081 acaaccgaac aggacctgaa agagtatttt agtacctttg gagaagttct tatggtgcag
     4141 gtcaagaaag atcttaagac tggtcattca aaggggtttg gctttgttcg ttttacggaa
     4201 tatgaaacac aagtgaaagt aatgtcacag cgacatatga tagatggacg atggtgtgac
     4261 tgcaaacttc ctaattctaa gcaaagccaa gatgagcctt tgagaagcag aaaagtgttt
     4321 gtggggcgct gtacagagga catgactgag gatgagctgc gggagttctt ctctcagtac
     4381 ggggatgtga tggatgtctt catccccaag ccattcaggg cctttgcctt tgttacattt
     4441 gcagatgatc agattgcgca gtctctttgt ggagaggact tgatcattaa aggaatcagc
     4501 gttcatatat ccaatgccga acctaagcac aatagcaata gacagttaga aagaagtgga
     4561 agatttggtg gtaatccagg tggctttggg aatcagggtg gatttggtaa tagcagaggg
     4621 ggtggagctg gtttgggaaa caatcaaggt agtaatatgg gtggtgggat gaactttggt
     4681 gcgttcagca ttaatccagc catgatggct gccgcccagg cagcactaca gagcagttgg
     4741 ggtatgatgg gcatgttagc cagccagcag aaccagtcag gcccatcggg taataaccaa
     4801 aaccaaggca acatgcagag ggagccaaac caggccttcg gttctggaaa taactcttat
     4861 agtggctcta attctggtgc agcaattggt gggggatcag catccaatgc agggtcgggc
     4921 agtggtttta atggaggctt tggctcaagc atggattcta agtcttctgg ctggggaatg
     4981 tagacccagc tttcttgtac aaagtggttg atcctctaga ctgagaactt cagggtgagt
     5041 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
     5101 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
     5161 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     5221 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     5281 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
     5341 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     5401 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     5461 ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     5521 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     5581 gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
     5641 catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     5701 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     5761 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5821 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5881 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5941 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     6001 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     6061 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     6121 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga
     6181 actcggcgcc ctgcccgtcc caccaggtca acaggcggta accggcctct tcatcgggaa
     6241 tgcgcgcgac cttcagcatc gccggcatgt ccccctggcg gacgggaagt atccagctcg
     6301 accaagctgg ccgcgtcgag tgaagacgaa agggcctcgt gatacgccta tttttatagg
     6361 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag tcgagggatc tccataagag
     8401 aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg ctagtaaaac
     8461 atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa atataaaaaa
     8521 aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag ggggctgttt
     8581 catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca tatgggcata
     8641 tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg ccctcccata
     8701 tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga aaataaattt
     8761 cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat aatttttggc
     8821 agagggaaaa agatc
//
