LOCUS       sequence-214934-        8857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses alpha-helix component of opto-RGS2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Hannanta-Anan P, Chow BY
  TITLE     Optogenetic Inhibition of Galphaq Protein Signaling Reduces Calcium 
            Oscillation Stochasticity.
  JOURNAL   ACS Synth Biol. 2018 Jun 15;7(6):1488-1495. doi: 
            10.1021/acssynbio.8b00065. Epub 2018 Jun 4.
  PUBMED    29792810
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00065"; journalName: "ACS Synth Biol"; date: 
            "2018-06-15- 15"; volume: "7"; issue: "6"; pages: "1488-1495"
COMMENT     Sequence Label: pLJM1-NtermRGS2-GFP-CIBN
FEATURES             Location/Qualifiers
     source          1..8857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(645..647,648..1358)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(645..647,648..1358)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(687..708)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(948..967)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1295..1316
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1380..1889
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     misc_feature    1940..2057
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2106..2616
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2535..2553
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2638..3237
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2638..2657)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3134..3154
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3365..3598
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3670..3791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3707..3726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3761..3780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3831..3966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3986..4005)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3987..4005)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4015..4032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4015..4031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4024..4046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4173..4628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4260..4279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4470..4491
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4654..4758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4759..4827,4828..5619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4977..4996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5790..6378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6279..6298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6532..6549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6666..6687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6702..6707,6708..6725,6726..6732)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6740..6756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6745..6767
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6764..6780
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6764..6780
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6799..6819
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6801..6819
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6847..7073
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7074..7254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7301..7426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7919..8152
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8337..8381
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8679..8699
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcatgcaaag tgctatgttc ttggctgttc aacacgactg cagacccatg gacaagagcg
      421 caggcagtgg ccacaagagc gaggagaagc gagaaaagat gaaacggacc cttttaaaag
      481 attggaagac ccgtttgagc tacttcttac aaaattcctc tactcctggg aagcccaaaa
      541 ccggcaaaaa aagcaaacag caagctttca tcaagccttc tcctgaggaa gcacagctgt
      601 ggtcagaagc atctagagaa ttcgcggccg ctcagctaac tagtgtgagc aagggcgagg
      661 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      721 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      781 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      841 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      901 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      961 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1021 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1081 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1141 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1201 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1261 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1321 ccgccgggat cactctcggc atggacgagc tgtataagac cggtggtgga tcacccggga
     1381 tgaatggagc tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc
     1441 gccaaagggc tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct
     1501 ttgccgattc ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt
     1561 tgaatcttcc gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt
     1621 cgccggaaac gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta
     1681 aggattgtaa tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag
     1741 gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga
     1801 agatgaaaca caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga
     1861 cgaaggaatt ggagaaaacg gattatattt aagtcgacca attctcgacc tcgagacaaa
     1921 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     1981 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2041 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
     2101 tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2161 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2221 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     2281 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     2341 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     2401 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     2461 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     2521 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     2581 tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga gcttaccatg
     2641 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     2701 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     2761 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     2821 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     2881 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     2941 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     3001 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     3061 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     3121 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     3181 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     3241 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
     3301 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     3361 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     3421 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3481 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3541 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3601 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     3661 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3721 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3781 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     3841 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3901 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3961 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4021 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4081 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4141 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4381 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4501 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4621 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4681 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4741 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4801 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4861 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4921 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4981 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5041 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5101 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5161 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5221 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5281 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5341 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5401 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5461 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5521 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5581 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5641 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5701 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5761 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5821 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5881 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5941 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6001 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6061 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6121 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6181 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6241 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6301 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6361 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6421 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6481 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6541 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6601 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6661 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6721 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6781 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     6841 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     6901 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     6961 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7021 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7261 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7321 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7381 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7441 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7501 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7561 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7621 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7681 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7741 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     7801 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     7861 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     7921 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     7981 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8041 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8101 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8161 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8221 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8281 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8341 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8401 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8461 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8521 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8581 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     8641 gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc
     8701 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     8761 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     8821 ttgcagtttt aaaattatgt tttaaaatgg actatca
//
