LOCUS       sequence-214944-       14196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone, including mKate, to be used to make constructs to
            be inserted as a single copy at a site on Chr I..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank 
            JJ, Pujol N
  TITLE     Modulatory upregulation of an insulin peptide gene by different 
            pathogens in C. elegans.
  JOURNAL   Virulence. 2018 Dec 31;9(1):648-658. doi: 
            10.1080/21505594.2018.1433969.
  PUBMED    29405821
REFERENCE   2  (bases 1 to 14196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/21505594.2018"; journalName: "Virulence"; date: 
            "2018-12-31- 31"; volume: "9"; issue: "1"; pages: "648-658"
COMMENT     Sequence Label: pSO5
FEATURES             Location/Qualifiers
     source          1..14196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1567..1600)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1600..1611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3033..3356)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5274..5298
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5301..6326
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5301..6326
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6327..6351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6704..6737)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7107..7123
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9724..10029
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(11571..11587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11571..11587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11584..11606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11595..11611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11619..11625,11626..11643,11644..11649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11664..11685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11746..12334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11826..11845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      12445..12954
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12532..12551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12738..12759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(12996..13787,13788..13856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13619..13638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13857..13961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14167..14189
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(14182..14196,1..2)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttgatcagt catccaagtg gggatcagga agaagttgat aaagtgattc aaaaactcga
       61 ggagctcaac acagcgatga acccaatttt gagagcaaca ctgacgaaga actatgacgc
      121 gagggtaggc actaccttga agctatccac ctgcctgcct gccgcctatt tccatgtgca
      181 gtaggaacgt gtgtaggtat aggcaggcat tatcgcgtct cctttcgaac tacctagtac
      241 ctagtaggta gtttgaaggg aggcgcgata atgcctgcct atgtctacac atgtgcctac
      301 tgcgcatgga aataggcggt agtcaggcag gtagatagcc tcataaaatt caatacttcc
      361 ttttcagaaa gaatgctgga atgtattcaa agaggagtca ctcttcccat tgctcagaga
      421 atatgaagag ttgctcggcg agcaaaagtt catgattgga gatcgatatt catgggctga
      481 catcgctgtc gtcgagtttc taactcggtg tcagcagtgc tacgatagct tctatctggc
      541 tcatttcgag aggctccgaa ctttttgcca gacttttgag tctcttccgc acattcgtcc
      601 ctacattcag ggacgtgttg attcatttat ctaatttttt aaaatggata tttttcgttt
      661 tttatttgtt tgttttgctg ataattcaat aaagttacgc aacgaatttt agacatttat
      721 gaaaaccgaa aatcgacaaa aatattttaa gaaaaatagc agcaaaattc aatattttat
      781 caaattttgt ttatggtaat gagaaagaaa acatacaata tttttctaag aagaagaaaa
      841 atgaccagaa ttttaagatt tttctcgaat atacgaaaaa ttgataaact aaaattttcg
      901 atttttccat ttattttgaa aaaaaaactc ttgacggttt tcatcatgcg aagaggtgag
      961 aaatattctg aaagttatgc attttgaaaa aattgcaaaa aaatacgtta aaattttttg
     1021 atctattgtt tttttttgaa aaccggaatt taaaatggaa ttttttttca aaaattaaaa
     1081 ttaaaattta aatatttcta gtgtttggat agtttttcaa aagctagatt taacccgcga
     1141 gatgtcgacc gctagtgtag cttacagatg gctgaagact tcgcaaaaat cttatcttag
     1201 aaacccgcga ggtgtcggcc gctaaaactt tgtaaacctg ccaacaaaat gcttcgcatt
     1261 gtctgaacag atgaaacctt gaaacaaaac atatacaatt gaaatcccgt aaagtgtcgg
     1321 gcgcaagaac tgcgattagt tgtataattg gactagctca aatttgacca aaatctcatt
     1381 tttagacctc taagatgtcg gccactataa ctttattcaa cttgaaactg caaaaaaact
     1441 tatcaactgt atagattcaa gtattatgtc aagtattggt tttagagcta cagtacactc
     1501 tgataacaaa atctgcagaa atacctccct gtcaattccc aaaatacggt agcaaactca
     1561 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1621 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1681 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1741 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1801 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1861 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1921 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1981 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     2041 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2101 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2161 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2221 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2281 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2341 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2401 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2461 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2521 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2581 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2641 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2701 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2761 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2821 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2881 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2941 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     3001 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3061 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3121 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3181 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3241 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3301 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3361 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3421 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3481 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3541 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3601 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3661 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3721 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3781 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3841 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3901 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3961 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     4021 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4081 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4141 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4201 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4261 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4321 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4381 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4441 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4501 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4561 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4621 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4681 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4741 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4801 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4861 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4921 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4981 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     5041 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5101 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5161 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5221 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5281 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5341 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5401 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5461 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5521 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5581 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5641 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5701 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5761 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5821 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5881 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5941 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6001 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6061 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6121 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6181 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6241 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6301 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6361 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6421 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6481 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6541 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6601 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6661 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6721 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6781 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6841 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6901 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6961 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     7021 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7081 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcggccg ctacccgaag
     7141 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     7201 tatcagttgc tcaaggcata tatgatgtca atatctccgg ttcggtaagc acaaccatgc
     7261 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     7321 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc cgacgagaac aggggctggt
     7381 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     7441 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     7501 cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa
     7561 actggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     7621 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     7681 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggttcgaat cgatggtctc
     7741 cgagctcatt aaagaaaaca tgcatatgaa gctctacatg gagggaaccg tcaacaacca
     7801 ccacttcaag tgcacctccg agggagaggg aaagccatac gagggaaccc aaaccatgcg
     7861 tatcaaggcc gtcgagggag gaccactccc attcgccttc gacatcctcg ccacctcctt
     7921 catgtacgga tccaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     7981 ttttcagacc ttcatcaacc acacccaagg aatcccagac ttcttcaagc aatccttccc
     8041 agagggattc acctgggagc gtgtcaccac ctacgaggac ggaggagtcc tcaccgccac
     8101 ccaagacacc tccctccaag acggatgcct catctacaac gtcaaggtaa gtttaaacag
     8161 ttcggtacta actaaccata catatttaaa ttttcagatc cgtggagtca acttcccatc
     8221 caacggacca gtcatgcaaa agaagaccct cggatgggag gcctccaccg agaccctcta
     8281 cccagccgac ggaggacttg agggacgtgc cgacatggcc ctcaagctcg tcggaggagg
     8341 acacctcatc tgcaacctca aggtaagttt aaacatgatt ttactaacta actaatctga
     8401 tttaaatttt cagaccacct accgttccaa gaagccagcc aagaacctca agatgccagg
     8461 agtctactac gtcgaccgtc gtcttgagcg tatcaaggag gccgacaagg agacctacgt
     8521 cgagcaacac gaggtcgccg tcgcccgtta ctgcgacctc ccatccaagc tcggacaccg
     8581 tggtggagga ggatctggag gaggaggatc catggtttcc gagttgatca aggagaatat
     8641 gcacatgaaa ctctacatgg aaggaaccgt caacaatcat catttcaaat gtacttcaga
     8701 aggagaagga aaaccatacg agggaactca gaccatgaga atcaaggctg ttgagggagg
     8761 taagtttaaa aaatacgtgt tcataacttg atacgaatat aaattttcag gaccacttcc
     8821 attcgcgttc gacattcttg ccacttcgtt catgtatgga agcaagactt tcattaatca
     8881 cacacaaggg atcccggatt tcttcaagca atctttccca gaaggattca catgggagag
     8941 agttaccact tacgaggatg gaggagttct taccgccacc caagacacat ccctccaagg
     9001 taagtttaaa agattatagc aatgttcgtc taaatataat aaattttcag atggatgtct
     9061 gatctacaac gtcaagatca gaggagtcaa tttcccatca aatggaccag ttatgcagaa
     9121 gaagactttg ggctgggagg cttccaccga gaccctttac ccagcagatg gaggtctcga
     9181 gggccgcgct gatatggctc tcaaactcgt cggaggagga cacttgatct gcaatttaaa
     9241 aaccacctac cggtcgaagg taagtttaaa atcaattttt aatagcttat ataataatac
     9301 aaattttcag aagccagcca agaatctcaa aatgccagga gtttactatg ttgaccgtcg
     9361 tcttgagcgc attaaagagg ccgataagga aacgtacgtt gaacaacatg aagttgccgt
     9421 tgctcgatat tgtgatctcc catccaagct cggacaccgt gccgcggccg ctacccgaag
     9481 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     9541 tatcagttgc tcaaggcata tatgatgtca atatctccgg ttcggtaagc acaaccatgc
     9601 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     9661 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc cgacgagaac aggggctggt
     9721 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     9781 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     9841 cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa
     9901 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     9961 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
    10021 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgaat cgatgcgcga
    10081 tgcaagtcgg cgatttcttt gaagttttgt caaaagaaga gacaaggact tgatagatac
    10141 gctcatcaga gtgagattca ttgcaaatag tgtgtaaatc gtggagagac gagagaaaag
    10201 gtgtgagaag cttagaagat aaagaatagg aagaagaaca tatctcattt cctattttct
    10261 ctaatttttc gttcattttt ctagacatta cccatttgat ggtttttcaa tatggttatc
    10321 agggtactca ttttggtagt tttcaaagca atttggagtt tttttttaaa ttgtcaacta
    10381 caaaaacgca gcttaaaggt ccaaatatca tagtataaaa attctttaaa aaattccaaa
    10441 ttttatatga ttttttgaaa attggaagaa tgttagtttt tgccattttt ttggaagttt
    10501 ttctccggat tttatggtac ttatatttca aatataaatt tttgtaggaa ttcgagtatg
    10561 cgagagagct atagtttact aaaaaaaagt ggcaaaaact cagtaattgc cacttttttc
    10621 aaaaatgttt tgaaaaattt tactatgata tttggccatt ttggcaccat atgtacgtta
    10681 ctttttacga aacactctaa tttttgaaat ttgagtttca tgtttaatct tttgtataaa
    10741 atttttatgg tccttttgtg acaggataaa aaattgcaaa aaaatagaaa actttgtttc
    10801 gtgaaaacac ctagtttaaa aattgtgggc cacttagcga ttctagtcaa aaatcagaca
    10861 aatgtcagct gccgtaactt tttccggatc agggttatca aattttaatt ttaattgtaa
    10921 atgtagaaaa atgatcggca acattttgct aattgaaaat attttgaaaa ttgctatagt
    10981 acatattaat ctagtattaa tatagtatta atgttataat atcagttatg tgtttgaata
    11041 actcaagcgt aagtttccgt aaaatatctt taaattttgg taatttttta cggtttggta
    11101 taaatttttg cattttttga ttttctacac tttttttcga tttcgcattt tccgaatttc
    11161 tctgaaataa atcgaacaat cggaaaattt tcgagggcac ccttagccat gcagctcagg
    11221 aatttgactt tatgttccca ttcaaatttt tttaaaatta aataaaatgc taattgtaag
    11281 ataaattaca aaaattatta aatctcatca tccttatgat gatagtgcag tgcatgaata
    11341 tatttttcca aataggatat gattcgtcga gtagcttcac gtaatccgtt ttcttcaatc
    11401 accatttcga actgcaattt tttttttgga tttttgtaac aatattgtca taaattgtga
    11461 ttttgatatt ttgcgtagag catcagaacc tttctgcttt gaattttaat gggattaaaa
    11521 attataaaaa acttgcaccc taattaaagc gcgatacctg gtgacccatg gtcatagctg
    11581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattacatgt gagcaaaagg
    11701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    12001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    12061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    12121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12181 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12421 aaggatcttc acctagatcc ttttacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    12481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    12541 ctttcttccc ttcctttctc gccacgttcg ctttccccgt caagctctaa atcgggggct
    12601 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
    12661 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12721 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    12781 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    12841 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat
    12901 atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtaaatca
    12961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    13021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    13081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    13141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    13201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    13261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    13321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    13621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttgggaaacg ttcttcgggg
    13681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    13741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    13801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    13861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    13921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    13981 ccacctgacg tagttaacaa aaaaaagccc gccgaagcgg gctttattac caagcgaagc
    14041 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    14101 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    14161 ggttttccca gtcacgacgt tgtaaaacga cggcca
//
