LOCUS       sequence-214945-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone, including mKate, to be used to make constructs to
            be inserted as a single copy at a site on Chr II..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, 
            Ewbank JJ, Pujol N
  TITLE     Microtubule plus-end dynamics link wound repair to the innate immune
            response.
  JOURNAL   Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047.
  PUBMED    31995031
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-29"; volume: "9. pii: 45047. doi"; pages: " 
            10.7554/eLife.45047"
COMMENT     Sequence Label: pNP154
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(666..683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(666..682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1052..1085
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    1438..1462
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1463..2488)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1463..2488)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2491..2515)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4433..4756
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             6178..6189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    6189..6222
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     rep_origin      complement(7710..8298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7790..7809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8469..9260,9261..9329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9092..9111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9330..9434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9502..9520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9558..9580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10902..11207)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
ORIGIN
        1 ggttccctcc atgtagagct tcatatgcat gttttcttta atgagctcgg agaccatcga
       61 ttcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
      121 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
      181 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagttt catccccgat
      241 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag
      301 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      361 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      421 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
      481 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
      541 ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
      601 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtagcg
      661 gccgcactgg ccgtcgtttt acaggatgat cgacgccaac gtcgttgaat tttcaaattt
      721 taaatactga atatttgttt tttttcctat tatttattta ttctctttgt gttttttttc
      781 ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc tttccgtctc
      841 ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc
      901 tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat
      961 ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg
     1021 tttaaaagaa aacataaaaa tatatactag tataacttcg tataaggtat actatacgaa
     1081 gttattaaaa attgcggtca taaactgaaa cgtaacatat gataaggtat tttgtgtgcg
     1141 gttttttcta tgatgtttca aaaaaattta tcagaagtaa aaaacataag aagtgtacac
     1201 agaacattgt gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt
     1261 tttcgacttt ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag
     1321 gtgacttaaa agaagctaaa aaacaaagaa attaagagaa gttttttgat aataacaaaa
     1381 atagggggtg ggagcacagg gagaaagagc atgtagggat gttgaagagt aattggaccc
     1441 actttgtaca agaaagctgg gtctattcct ttgccctcgg acgagtgctg gggcgtcggt
     1501 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     1561 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     1621 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     1681 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     1741 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     1801 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     1861 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     1921 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     1981 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     2041 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     2101 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     2161 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     2221 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     2281 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     2341 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     2401 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     2461 acgtcgcggt gagttcaggc tttttcatgg agcctgcttt tttgtacaaa cttgcggcac
     2521 cgcctgacat attaccttaa aattcaaaaa ttaatttcag atcagtcata accaagaaaa
     2581 caaaataaac gttaggacag aaaattcaaa ttaattaaaa attatgctga ataagaaaat
     2641 tacatgcaaa gccttatttt cctcatagaa acagttgaat aacatatttt ttaattgaaa
     2701 acatagcaaa attcgtggta aaatttgtca aaaccttgga aaaaatcgaa aaaagaacat
     2761 ttcccgatgc attttcctat ttctcgttgc cgacaacgta taaacgttga atactcagtc
     2821 aacgaatttg gctgctggct tggactctcg cacggaagag acgcgtgtgt agatttacga
     2881 cgaaagcaaa aatgatcacc aaaaacggaa cgttgagctg gacggaaata gtggtaaagt
     2941 gacatgatta tagtttgaag atttctaatt tcacaattag agcaaatgtt gttcggtatt
     3001 tattttcaac ggtatttata ctattttcca cctttttcta gaacattcga gctgcttgtt
     3061 gcaaaaggag ggcgactcac attcggtaca tggaaaagta gtgtacacaa taaagagacc
     3121 cagatacatt ttccgtctgc gtctctttgc acccaccggg agtattttca aacgaatgca
     3181 tctaggacct tctagaacat tctgtaaggc tgcagaatgc gggtatataa ggaaagcggg
     3241 ctcagaggaa gccaacacgc tttgttctag tgcatctaaa aaacttcgaa aatatgtcaa
     3301 atcttctcac tgttcatcaa aatcttccag cacttccagt tgacgcgaca agtgatgaag
     3361 ttcgcaagaa cctcatggat atgttccgtg acagacaagc cttctccgag cacacctgga
     3421 agatgcttct ctcagtgtgc cgttcttggg ctgcctggtg caagcttaac aaccgcaagt
     3481 ggttcccagc cgagccagaa gacgttcgtg actacttgct ctacctccaa gctcgtggat
     3541 tggcggtgaa gacgatccaa caacatctcg gacagctcaa tatgctccat cgccgatctg
     3601 gacttccacg cccatcagat tctaatgccg tatctctcgt aatgagacgt atccgcaagg
     3661 aaaatgttga tgctggagag agagccaagc aagccctcgc cttcgaaagg taagtttaaa
     3721 tattaatgta ttcaatccta agacttgacg aaattttcag aaccgacttc gatcaggtcc
     3781 gttctcttat ggaaaattcc gatcgttgcc aggacatccg aaacctcgcc ttcctcggaa
     3841 ttgcttacaa cacacttctc cgtatcgccg aaattgcccg cattcgcgtc aaggatatta
     3901 gtcgcacaga cggaggaaga atgctcatcc acatcggacg tacaaaaacg ctggtctcga
     3961 ctgccggagt cgaaaaagcg ctctcccttg gagttaccaa gctcgttgag cgctggattt
     4021 cggtttccgg agtcgccgat gatccaaaca actacctgtt ctgtcgtgtt cgcaagaacg
     4081 gagttgctgc cccgagtgca acatcacaac tctcgactcg agctcttgaa ggtaagttta
     4141 aacaacatat tagctaagaa taagctgaaa ataaattttc aggaatcttc gaagctaccc
     4201 accgtctcat ctacggagcc aaggatgatt ccggacaacg ttacttggct tggtctggac
     4261 attccgcccg tgtcggagct gcccgtgaca tggctcgcgc tggagtttcc atcccagaga
     4321 ttatgcaagc cggaggttgg accaacgtca acattgtgat gaactatatt agaaacttgg
     4381 attctgaaac tggcgccatg gttcgtcttc tcgaagacgg tgattaagat aaatgcaaaa
     4441 tcctttcaag cattcccttc ttctctatca ctattctttc tttttgtcaa aaaattctct
     4501 cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag
     4561 tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt actttcacaa
     4621 tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa aatgcgagcg
     4681 ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac tttattgtgc
     4741 cgccaagaaa aaagtctcac tgaatctcaa atattttatt agaaaacacc aacaatttag
     4801 atatttctgt atccacggtt ggcgttgaca gcctctgatg gtggagactt gtctgggcac
     4861 tggcagtact cggcatcctt tcctggtctt ccgtctggtc cagtctctcc ctgggttccg
     4921 tctggtcctt gacgtccttg ctttccagat ggtccttctg gtccaacggt tcctggctct
     4981 cctggtggtc cgtgaagacc gttctgtcca gctggtcctg ttggtccttg atctcctctt
     5041 ggtccctttg gtcctggtct tccgattgga tgctcggcgt cgcgtccctt ctctcctggt
     5101 ggtcctggct ctccgatctt tccgatttga ccggatgatc cttgctctcc tggatgtcct
     5161 ggttgtccat ctcttcctgg gtttccattt tgtcctcttg gtcctggaag tccacgtggt
     5221 cctggtcttc caagagctcc tggtggtcca actggtcctt gtgggcaagt gaagcatccg
     5281 acagactcgc gttgtggagc gatatcatca gctccaactc ctggaacacc gtcaagtcct
     5341 ggaactccgt cgagtcctgg aactcctgga actccctttg gtccggatgg tccagctggg
     5401 cacttgttat cagcattgca gttgcaggtt ggtccatttc cattggctcc atttggagtc
     5461 tttggctggt tgaagactgg tggaactgct ggtggttgac ctggtggagc agatggggat
     5521 ccatcagcat gtggagttgg ggtagcgttg gtctcttcat attgacaacg gacacgcttg
     5581 ctagatcttc cgatgacaac tatgtccttc cacaatccat tggtagattg ctagaaaatt
     5641 aaaaattatt taaaattcaa aatataattt aactcactct gaacacattc atctcggtat
     5701 caagcttctc tctgaatcca tcaacctccg agtagatggt tgacatggtc atgaagcata
     5761 ccataagagt ggcgacagtg accgaagccg tcacataaca cgcaagtttt acagacatct
     5821 ctgaaattat caaatttagg atttcaaaat aattcggcca aagaaatgta actcacctag
     5881 tcttcgagaa gcagagactt gtcgatttcc taccggattc gtctcctttt attaggaaat
     5941 ctttccgcaa gttttcaagt tttttttgtt ttttttttac attaccgacg agacagtgtg
     6001 cgagacgcag agacgataaa agactggata agaacgggag ataaggagta cggatgtagg
     6061 tgaggaatgt ggccgaaagg acagatgggc tttattcggg aagattggtt gggaaatgaa
     6121 ccagagaaga atgactgaat aaagttgttg aaacttggtg agaatggaag atagacaatc
     6181 gagggcagat aacttcgtat aaggtatact atacgaagtt atacgaagtg agtttgctac
     6241 catcatggaa tcaggaggac atttcgacaa tgtcatataa tgtttgaaaa atgattttaa
     6301 caagggtctc attcgagata agcaatgctc tgagcaagtg gctcgtcgtc ttcaagaatc
     6361 tcttgttgat attcatttgg atcctgtttt gcacgaagca tgtgccatgg atattcagag
     6421 tttttgcaaa aaagttccgc caggtcaaac aatcttttta atttttatat gatacttcgg
     6481 gtgtattttt cttctttttc ctgttcattt tcgctttttt ctcacttttt tgtaacaaaa
     6541 tctctcactt ttctgtaagt cttaacaaaa gttcaattgg ctcgatcaag taaaatttcg
     6601 gccgtatgaa cgttttcaaa ggttttcatt tttttttaag ttcattcaca atatcatgaa
     6661 tctctcgtcc tttggtttcc ggtaagtagc gaaataatat aagcatactt ataaatgaag
     6721 gaattgaata tagaataagg aaagcataac taccaataac ggaatataga ggtaatactg
     6781 taaatgtact gataaccacc attacagtat tcattgaata tgccacagat tgagcaatac
     6841 tacgatgttt ttgaggtacc agttcagagg atataaacca cgaaattgga ccaactccag
     6901 ttctgaaatt tcattattcg attaaatcaa aatatatttg aaaaacctca cccataagta
     6961 taaccgtaga tgatcaatgc aactaggcat ccgtatttca tcgggtcaat cagaatacgt
     7021 atttttgcga agaatacgaa tgcaattaga gctaaattat tgagaaatgt gaagagaagg
     7081 agaagcttgc gtcgaccaac tctgtaaact tattcataca attttgtctt caaattttga
     7141 acgtttaaaa ctatcttatt caatgagtta gcgggttttt atagtgagtt agttcacgat
     7201 tatctaataa aactatgtcc aaacatccta actgcgaccg tattctcact aactcgtctt
     7261 aaaaacgttg agattatttt aaacccttct tcagtacaac tacagtactg atgcacaaat
     7321 acaataataa taatacattc agacatactc tgaacaatgt aacttgtttt tgttatactg
     7381 tggtaacatt tattgaaatg aaatataacc ttacttttca atcacaattc caccagtaat
     7441 tgttccagaa acataagcta aacccattgc agttgtactc cattcagcaa tttctgactc
     7501 aacatttgca ttttctagaa agaaagtaga ggataaaaga agagcccata atccgacagg
     7561 gccctgtgaa attgttatcc gctggtatca gctcactcaa aggcggtaat acggttatcc
     7621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaagaccagg
     7681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8581 tctgacccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9421 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9481 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     9541 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     9601 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     9661 cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca ccatatgcgg
     9721 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     9781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9901 cgacgtcacg tggaagaagt ggaggccttg ttgtcgatct acaaaattgt atggttgttt
     9961 gagattttcc ttgaaacgct ctcaatcttg gatatgatat aaattctgga taagatatga
    10021 tggatatgat ctatgtttga tagagtgcat ttgaaaggat aacggccctg cagacatttt
    10081 tgaaaaaata acaaacattt aattttctca ctcgtttagg ctattcccct atttttgata
    10141 ttccttcgca catatgaaaa ctactttttt tcgaaactgt taactccaga atttataaat
    10201 ctatagccct tacttgatta tttattatca tggttactca cctcatgtct ctttctcttt
    10261 tcaagaagtc ttcgttcata tctgttgtct ctttcctcct tttgaatctc tgctttgctc
    10321 ttcgccattg tttcctgaaa ataatgtaac ttgaattgtg taatatactt ttttaatttg
    10381 aatttggctt gtaacgcgga atcactacgt gcgggatcat ttcttactag aaaacccaga
    10441 aaatgccata tttcacttat ctcggggtca tttctaatta gaaaagctac aaaaaccatt
    10501 tctaattacg gtgactttag aactggtcga aatagctaat tagaaatggg tttttgctaa
    10561 ttagaggtga ctctatgtta tctgagtaag cctcgtttgt atgtaaactc acagtactaa
    10621 taaatgcaag acacccgggt ttgtctagat atgaaataat tgaaatatca attctgacag
    10681 acaataatgg taatcttgat aaggagttcc acgcccagga gaacacgtta gttttcttgt
    10741 ttttgattgc gtgcgttatt ttggagaaaa actcgatttt ttacaaaata attttttgaa
    10801 aggaacactg ttcaataagt tttgtctttt ttctcagttg tgatacggca tcgcgcatcg
    10861 attcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
    10921 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
    10981 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
    11041 atatgcacca ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc
    11101 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
    11161 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
    11221 gttctcgtcg gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
    11281 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
    11341 gcttaccgaa ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
    11401 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtag
    11461 cggccgcggc acggtgtccg agcttggatg ggagatcaca atatcgagca acggcaactt
    11521 catgttgttc aacgtacgtt tccttatcgg cctctttaat gcgctcaaga cgacggtcaa
    11581 catagtaaac tcctggcatt ttgagattct tggctggctt ctgaaaattt gtattattat
    11641 ataagctatt aaaaattgat tttaaactta ccttcgaccg gtaggtggtt tttaaattgc
    11701 agatcaagtg tcctcctccg acgagtttga gagccatatc agcgcggccc tcgagacctc
    11761 catctgctgg gtaaagggtc tcggtggaag cctcccagcc caaagtcttc ttctgcataa
    11821 ctggtccatt tgatgggaaa ttgactcctc tgatcttgac gttgtagatc agacatccat
    11881 ctgaaaattt attatattta gacgaacatt gctataatct tttaaactta ccttggaggg
    11941 atgtgtcttg ggtggcggta agaactcctc catcctcgta agtggtaact ctctcccatg
    12001 tgaatccttc tgggaaagat tgcttgaaga aatccgggat cccttgtgtg tgattaatga
    12061 aagtcttgct tccatacatg aacgaagtgg caagaatgtc gaacgcgaat ggaagtggtc
    12121 ctgaaaattt atattcgtat caagttatga acacgtattt tttaaactta cctccctcaa
    12181 cagccttgat tctcatggtc tgagttccct cgtatggttt tccttctcct tctgaagtac
    12241 atttgaaatg atgattgttg acggttcctt ccatgtagag tttcatgtgc atattctcct
    12301 tgatcaactc ggaaaccatg gatcctcctc ctccagatcc tcctccacca cggtgtccga
    12361 gcttggatgg gaggtcgcag taacgggcga cggcgacctc gtgttgctcg acgtaggtct
    12421 ccttgtcggc ctccttgata cgctcaagac gacggtcgac gtagtagact cctggcatct
    12481 tgaggttctt ggctggcttc ttggaacggt aggtggtctg aaaatttaaa tcagattagt
    12541 tagttagtaa aatcatgttt aaacttacct tgaggttgca gatgaggtgt cctcctccga
    12601 cgagcttgag ggccatgtcg gcacgtccct caagtcctcc gtcggctggg tagagggtct
    12661 cggtggaggc ctcccatccg agggtcttct tttgcatgac tggtccgttg gatgggaagt
    12721 tgactccacg gatctgaaaa tttaaatatg tatggttagt tagtaccgaa ctgtttaaac
    12781 ttaccttgac gttgtagatg aggcatccgt cttggaggga ggtgtcttgg gtggcggtga
    12841 ggactcctcc gtcctcgtag gtggtgacac gctcccaggt gaatccctct gggaaggatt
    12901 gcttgaagaa gtctgggatt ccttgggtgt ggttgatgaa ggtctgaaaa tttaaataat
    12961 cagggttagt tagtatatat atgtttaaac ttaccttgga tccgtacatg aaggaggtgg
    13021 cgaggatgtc gaaggcgaat gggagtggtc ctccctcgac ggccttgata cgcatggttt
    13081 gggttccctc gtatggcttt ccctctccct cggaggtgca cttgaagtgg tggttgttga
    13141 c
//
