LOCUS       sequence-214948-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of 
            TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Li M, Yu JSL, Tilgner K, Ong SH, Koike-Yusa H, Yusa K
  TITLE     Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 
            Regulation in Naive Pluripotency Maintenance and Dissolution.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):489-502. doi: 
            10.1016/j.celrep.2018.06.027.
  PUBMED    29996108
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "489-502"
COMMENT     Sequence Label: pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     328..348
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             391..571
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    618..743
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1242..1475
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1660..1704
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2002..2119
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2194..2442
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2194..2214
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2365..2384
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2571..3070
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2606..2629)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3003..3022
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3110..3706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3110..3129)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3606..3626
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3725..3778
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3779..4480
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4540..4560)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4958..4969)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5421..5438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5427..5651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5677..5697)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5677..5695)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5716..5732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5716..5732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5729..5751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5740..5756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5764..5770,5771..5788,5789..5794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5809..5830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5947..5964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6118..6706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6198..6217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6877..7668,7669..7737))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7500..7519
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7738..7842)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7868..8323)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8005..8026)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8217..8236
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8450..8472
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8464..8481
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8465..8481
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8491..8510
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8491..8509
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
       61 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      121 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      181 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      241 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      301 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      361 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      421 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      481 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      541 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      601 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      661 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      721 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      781 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      841 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      901 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
      961 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1021 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1081 cggccggccg cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1141 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1201 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1261 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1321 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1381 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1441 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1501 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1561 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1621 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1681 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1741 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1801 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1861 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1921 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1981 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2041 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2101 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttagt
     2161 accgggccct acgcgtccaa ggtcgggcag gaagagggcc tatttcccat gattccttca
     2221 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     2281 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     2341 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     2401 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtagcgca gcgctcgcaa
     2461 ccgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     2521 acttgaaaaa gtggcaccga gtcggtgctt tttttggatc caattctacc gggtagggga
     2581 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     2641 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     2701 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     2761 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     2821 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     2881 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     2941 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     3001 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     3061 gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca tgaccgagta
     3121 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     3181 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     3241 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     3301 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     3361 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     3421 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     3481 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     3541 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     3601 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     3661 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccggcg gcgggtccgg
     3721 aggagagggc agaggaagtc tcctaacatg cggtgacgtg gaggagaatc ctggcccaat
     3781 gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa
     3841 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     3901 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
     3961 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     4021 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt
     4081 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     4141 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     4201 ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
     4261 cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa
     4321 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
     4381 aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata
     4441 ctgcgacctc cctagcaaac tggggcacaa gcttaattga gcggccgctc aacctctgga
     4501 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4561 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4621 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4681 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4741 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4801 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4861 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     4921 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4981 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctacgcc ttcgccctca
     5041 gacgagtcgg atctcccttt gggccgcctc cccgcaggta cctttaagac caatgactta
     5101 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5161 tcactcccaa agaagtcaag atctgctttt tgcctgtact gggtctctct ggttagacca
     5221 gagtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5281 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5341 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5401 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5461 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5521 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5581 ggggcaggac agcaaggggg aggattggga agtcaatagc aggcatgctg gggatgcggt
     5641 gggctctatg ggcggccgtt aattaagctt ttgttccctt tagtgagggt taattgcgcg
     5701 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5761 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5821 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5881 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6541 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7861 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7921 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7981 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8041 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8101 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8161 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8221 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8281 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8341 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8401 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8461 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgact
     8521 agtacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8641 tgggtggagt
//
