LOCUS       sequence-214950-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of 
            TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Li M, Yu JSL, Tilgner K, Ong SH, Koike-Yusa H, Yusa K
  TITLE     Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 
            Regulation in Naive Pluripotency Maintenance and Dissolution.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):489-502. doi: 
            10.1016/j.celrep.2018.06.027.
  PUBMED    29996108
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "489-502"
COMMENT     Sequence Label: pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        172..370
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     325..345
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             388..568
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    615..740
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1239..1472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1657..1701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1999..2116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2191..2439
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2191..2211
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2362..2381
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2568..3067
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2603..2626)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3000..3019
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3107..3703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3107..3126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3603..3623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3722..3775
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3776..4477
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4537..4557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4955..4966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5418..5435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5424..5648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5674..5694)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5674..5692)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5713..5729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5713..5729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5726..5748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5737..5753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5761..5767,5768..5785,5786..5791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5806..5827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5944..5961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6115..6703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6195..6214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6874..7665,7666..7734))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7497..7516
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7735..7839)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7865..8320)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8002..8023)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8214..8233
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8447..8469
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8461..8478
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8462..8478
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8488..8507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8488..8506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
       61 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      121 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      181 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      241 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      301 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      361 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
      421 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      481 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      541 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      601 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      661 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      721 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      781 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      841 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
      901 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
      961 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1021 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1081 ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1141 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1201 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1261 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1321 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1381 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1441 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1501 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1561 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1621 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1681 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1741 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1801 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1861 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1921 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     1981 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2041 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2101 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     2161 gggccctacg cgtccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat
     2221 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
     2281 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     2341 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     2401 ttcttggctt tatatatctt gtggaaagga cgaaacaccg cagggatttc atcgtgcaag
     2461 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     2521 tgaaaaagtg gcaccgagtc ggtgcttttt ttggatccaa ttctaccggg taggggaggc
     2581 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     2641 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     2701 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     2761 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     2821 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     2881 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     2941 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     3001 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     3061 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
     3121 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     3181 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     3241 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     3301 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3361 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3421 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     3481 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     3541 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     3601 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     3661 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggcggcg ggtccggagg
     3721 agagggcaga ggaagtctcc taacatgcgg tgacgtggag gagaatcctg gcccaatgag
     3781 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
     3841 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     3901 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
     3961 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
     4021 gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg ggggcgtgct
     4081 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     4141 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     4201 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
     4261 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
     4321 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
     4381 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
     4441 cgacctccct agcaaactgg ggcacaagct taattgagcg gccgctcaac ctctggatta
     4501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4861 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     4921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctacgccttc gccctcagac
     5041 gagtcggatc tccctttggg ccgcctcccc gcaggtacct ttaagaccaa tgacttacaa
     5101 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5161 ctcccaaaga agtcaagatc tgctttttgc ctgtactggg tctctctggt tagaccagag
     5221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5401 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5581 gcaggacagc aagggggagg attgggaagt caatagcagg catgctgggg atgcggtggg
     5641 ctctatgggc ggccgttaat taagcttttg ttccctttag tgagggttaa ttgcgcgctt
     5701 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5761 caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6541 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7861 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7921 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7981 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8041 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8101 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8161 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8221 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8281 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     8341 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8401 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8461 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgactagt
     8521 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8581 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8641 gtggagtatt
//
