LOCUS       sequence-214958-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C:G-to-T:A base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP,
            Raguram A, Liu DR
  TITLE     Improving cytidine and adenine base editors by expression 
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172."
COMMENT     Sequence Label: pCMV_BE4max_P2A_GFP
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             302..550
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             581..829
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             872..892
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
       61 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
      121 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
      181 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
      241 acggatcgac ctgtctcagc tgggaggtga cagcggcggg agcggcggga gcggggggag
      301 cactaatctg agcgacatca ttgagaagga gactgggaaa cagctggtca ttcaggagtc
      361 catcctgatg ctgcctgagg aggtggagga agtgatcggc aacaagccag agtctgacat
      421 cctggtgcac accgcctacg acgagtccac agatgagaat gtgatgctgc tgacctctga
      481 cgcccccgag tataagcctt gggccctggt catccaggat tctaacggcg agaataagat
      541 caagatgctg agcggaggat ccggaggatc tggaggcagc accaacctgt ctgacatcat
      601 cgagaaggag acaggcaagc agctggtcat ccaggagagc atcctgatgc tgcccgaaga
      661 agtcgaagaa gtgatcggaa acaagcctga gagcgatatc ctggtccata ccgcctacga
      721 cgagagtacc gacgaaaatg tgatgctgct gacatccgac gccccagagt ataagccctg
      781 ggctctggtc atccaggatt ccaacggaga gaacaaaatc aaaatgctgt ctggcggctc
      841 aaaaagaacc gccgacggca gcgaattcga gcccaagaag aagaggaaag tcggaagcgg
      901 agctactaac ttcagcctgc tgaagc
//
