LOCUS       sequence-215027-       10277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian constitutive expression of HA-tagged Tetracyclin repressor
            protein and Myc-tagged blasticidin deaminase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Elegheert J, Behiels E, Bishop B, Scott S, Woolley RE, Griffiths SC,
            Byrne EFX, Chang VT, Stuart DI, Jones EY, Siebold C, Aricescu AR
  TITLE     Lentiviral transduction of mammalian cells for fast, scalable and 
            high-level production of soluble and membrane proteins.
  JOURNAL   Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 
            10.1038/s41596-018-0075-9.
  PUBMED    30455477
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 
            10.1038/s41596-018-0075-9."
COMMENT     Sequence Label: pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc
FEATURES             Location/Qualifiers
     source          1..10277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(21..39)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(22..39)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(329..348)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(562..581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     681..703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(741..759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        827..931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(932..1000,1001..1792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1150..1169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1963..2551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2452..2471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2705..2722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2792..2812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2797..3126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2977..3112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4261..4326
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4456..4476
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4901..5035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4938..4957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4992..5011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5203..5836
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5883..6008
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6505..6738
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6923..6967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7252..7369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7490..7869
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7870..8073
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8024..8044
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             8138..8758
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(8211..8230)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             8762..8788
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8792..8812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8822..8878
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8879..9274
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             9284..9313
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    9342..9930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9395..9415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9813..9824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10040..10273
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
       61 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
      121 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
      181 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
      241 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
      301 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      361 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      421 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      481 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      541 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      721 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      781 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      901 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      961 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1021 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1081 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1141 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1201 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1261 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1321 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1381 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1441 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1501 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1561 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1621 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1681 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1741 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2641 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2701 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     2761 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     2821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3121 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     3181 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     3241 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     3301 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     3361 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     3421 tattgccgta agccgtggcg gtctcgtacc gggtgcgtta ctggcgcgtg aactgggtat
     3481 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     3541 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     3601 ttccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     3661 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     3721 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     3781 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     3841 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     3901 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     3961 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     4021 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     4081 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     4141 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     4201 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     4261 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     4321 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     4381 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     4441 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     4501 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     4561 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     4621 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     4681 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     4741 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     4801 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4861 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     5041 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     5101 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     5161 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     5221 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5281 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     5341 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     5401 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     5461 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     5521 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     5581 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     5641 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5821 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     5881 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     5941 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     6001 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6061 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6121 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6181 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6241 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6301 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     6361 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6421 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6481 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6541 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6601 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6661 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6721 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6781 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6841 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6901 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6961 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7021 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7081 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7141 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7201 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     7261 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7321 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     7381 cagggacagc agagatccag tttggatcga taagcttgat atcgaattgc tgcagccccg
     7441 atagtcgact acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     7501 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7561 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7621 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7681 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7741 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7801 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7861 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     7921 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7981 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     8041 cgtgtacggt gggaggtcta tataagcaga gctctcgtcg tcgtgctcgt ttagtgaacc
     8101 gtctgatctc aacaagctgt ctagagaatt cgccaccatg tctagattag ataaaagtaa
     8161 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     8221 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     8281 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     8341 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     8401 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     8461 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     8521 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     8581 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     8641 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     8701 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     8761 gtatccatat gatgttccag attatgctgg acctaagaag aagaggaagg ttggaagcgg
     8821 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctat
     8881 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     8941 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     9001 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     9061 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     9121 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     9181 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     9241 tgaattgctg ccctctggtt atgtgtggga gggcggtggt tccgaacaga agctgatctc
     9301 cgaagaagac ctgtaaagcg gccgctcacg tacgtatcga taatcaacct ctggattaca
     9361 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9421 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9481 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9541 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9601 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9661 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9721 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     9781 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9841 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9901 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgtccacga gggaattaat
     9961 tcgagctccg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10021 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
    10081 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10141 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10201 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10261 aaaatctcta gcagcaa
//
