LOCUS       sequence-215077-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sat2 for Nourseothricin resistance selection of C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Obinata H, Sugimoto A, Niwa S
  TITLE     Streptothricin acetyl transferase 2 (Sat2): A dominant selection 
            marker for Caenorhabditis elegans genome editing.
  JOURNAL   PLoS One. 2018 May 9;13(5):e0197128. doi: 
            10.1371/journal.pone.0197128. eCollection 2018.
  PUBMED    29742140
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-9"; volume: "13(5):e0197128. doi"; pages: " 
            10.1371/journal.pone.0197128. eCollection 2018"
COMMENT     Sequence Label: pSN444
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2215..2237)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2337..2356
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2438..2456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2524..2628
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2629..2697,2698..3489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2847..2866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3660..4248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4149..4168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4402..4419
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4536..4557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4572..4577,4578..4595,4596..4602)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4610..4626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4615..4637
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4634..4645,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4634..4645,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgct gggccatggc accttaacat ttctaaattt
       61 tagtttaata aaaaaatttt aaaataatac gagttttgag actaactttc aataaaaacc
      121 aaaaaattgc ttcaaaaaag agctcgcgcc gatttacgat aaacacagcg cgctacgaca
      181 ctccgaaaac cgcgccgcac cattacattc tccgccccac cgcacggttt gcggaaggcg
      241 atgaaattgc tgggcctaaa attttaaccc tttttttccc gtttttcaac tgttttcccg
      301 attttttcga tatttttttc aggtttttag ttagaaaagt atataaaaat tgcattattt
      361 gtagttttcg ctcgtaattt gtgatagaaa acggccaatt tgcctagatt tgtgattcta
      421 ccaagtggaa tttttgggaa aatatcaata ttccacgatt tttattcaaa tcataacgtt
      481 tcgaaattat cagcttaaat aaaactaaaa taaataataa aatactaaaa accctgaatt
      541 tctcggttct cggttcagct tgagttcgtt ctatacacgt tatcgatttt tgtcatttcg
      601 tttaatcatg ggttttgatt cattttaaat atgaaatttg atttattcct cgtttcgaag
      661 ttggtttgtc ttgtccggcc ggtaaacaaa tgttattaaa aaaaataata aatttcgaaa
      721 tttttcgtaa tgttccgcaa tcgtgttttt ccgtttatct cgtcttccct tccttcaaat
      781 atactattta ttttctcaat tcacatattt tcagccagcc ctgctcaaca agtggaataa
      841 aaggcgcgcc atgaagatct ccgtcatccc agagcaagtc gccgagaccc tcgacgccga
      901 gaaccacttc atcgtccgtg aggtcttcga cgtccacctc tccgaccaag gattcgagct
      961 ctccacccgt tccgtctccc cataccgtaa ggactacatc tccgacgacg actccgacga
     1021 ggactccgcc tgctacggag ccttcatcga ccaagagctc gtcggaaaga tcgagctcaa
     1081 ctccacctgg aacgacctcg cctccatcga gcacattgtc gtctcccaca cccaccgtgg
     1141 aaagggagtc gcccactccc tcatcgagtt cgccaagaag tgggccctct cccgtcaact
     1201 cctcggaatc cgtctcgaga cccaaaccaa caacgtccca gcctgcaacc tctacgccaa
     1261 gtgcggattc accctcggag gaatcgacct cttcacctac aagacccgtc cacaagtctc
     1321 caacgagacc gccatgtact ggtactggtt ctccggagcc caagacgacg cctaaggtac
     1381 catggtattg atatctgagc tccgcatcgg ccgctgtcat cagatcgcca tctcgcgccc
     1441 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     1501 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     1561 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     1621 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     1681 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     1741 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     1801 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     1861 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     1921 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     1981 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     2041 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     2101 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttctac
     2161 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     2221 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     2281 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     2341 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     2401 aaggagaaaa taccgcatca ggcggcctta attaagggcc tcgtgatacg cctattttta
     2461 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     2521 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2581 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2641 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2701 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2761 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2821 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2881 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2941 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3001 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3061 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3121 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3181 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3241 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3301 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3361 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3421 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3481 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4321 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4381 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4441 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4501 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4561 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4621 taacaatttc acacaggaaa cagct
//
